Development and characterization of microsatellite markers for Chinese bayberry (Myrica rubra Sieb. &amp; Zucc.)

Zhang, Shui Ming; Xu, Chang; Chen, Kun Song; Wang, Guo Yun

doi:10.1007/s10592-008-9804-x

Cited by 14 publications

(18 citation statements)

References 3 publications

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“…Genomic DNA for all accessions was isolated from the young leaves with cetyltrimethylammonium bromide using a modification of the method described by Zhang et al (2009b). DNA concentrations were estimated spectrophotometrically at 260 nm and were standardized at 20 ng/μl.…”

Section: Plant Materialsmentioning

confidence: 99%

“…PIC ¼ 1À P P ij À Á 2 , where the P ij is the frequency of the ith pattern revealed by the jth primer summed across all patterns revealed by the primers (Botstein et al 1980). Zhang et al (2009b), and the last seven are from Terakawa et al (2006) Ta annealing temperatures, bp size range, A number of alleles detected, Ho observed heterozygosity, He expected heterozygosity, PIC polymorphism information content a EST-SSRs developed from the expressed sequence tags Genetic similarity among all the accessions was calculated according to Dice's coefficients using Nei's coefficient index (Nei 1972) with the software NTSYSpc 2.1 (Rohlf 2000), and a Mantel test was used to compare similarity matrices calculated from AFLP (Zhang et al 2009a) and SSRs data in 99 common accessions with the same software.…”

Section: Pcr Reactionmentioning

confidence: 99%

“…The accessions with other colors as well as 16 androphytes had a similar clustering pattern. "Dongkui" bearing the biggest fruit, with an average weight of 20-25 g, was closely related to "Yuyaobai" with an average fruit weight of 10-12 g. Two genomic SSRs, MYBSSR1 and MYBSSR2, were developed by genomic cloning using the conserved R2R3 domain degenerated primer (Zhang et al 2009b). There were signs that these two SSRs were related to color.…”

Section: Genetic Relationships Among Chinese Bayberry Accessionsmentioning

confidence: 99%

“…The efficiency of using microsatellite markers for testing genetic relationships has been demonstrated in many different fruit tree species, including peach (Testolin et al 2000;Xie et al 2010), apple (Galli et al 2005), citrus (Jannati et al 2009;Mariãngela et al 2010), olive (Sónia et al 2009), avocado (Ying et al 2009), and loquat (He et al 2010). Up to now, only 24 SSR markers have been developed, 13 by Terakawa et al (2006) and 11 by Zhang et al (2009b).…”

Section: Introductionmentioning

confidence: 99%

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Cultivar Identification and Genetic Diversity of Chinese Bayberry (Myrica rubra) Accessions Based on Fluorescent SSR Markers

et al. 2010

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Abstracts A collection of 122 Chinese bayberry accessions and one wax myrtle (Myrica cerifera L.) were analyzed with 14 polymorphic simple sequence repeats (SSRs). The average number of alleles per locus was 9.3, and polymorphism information content varied from 0.07 to 0.83, with a mean value of 0.62. The genetic relationships among the 123 accessions were analyzed using the unweighted pair-group method with arithmetic mean (UPGMA). The similarity among all the accessions, based on Dice's coefficient, varied from 0.78 to 0.99, and 0.74 between the Chinese bayberries and wax myrtle. A set of 122 Chinese bayberries clustered into four groups, with the first group further divided into six subgroups. The accessions originating from the same geographical region were more closely related than those from different regions, although extensive gene flow has taken place. The Mantel test, used to compare similarity matrices calculated from AFLP and SSR data, showed that their combination could provide information on the genetic relationship among the Chinese bayberry accessions. Ten selected SSR markers were able to distinguish most accessions, and multiplex PCR systems were developed. In addition, we found that SSRs developed from Chinese bayberry are transferable to M. cerifera.

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Section: Plant Materialsmentioning

confidence: 99%

Section: Pcr Reactionmentioning

confidence: 99%

Section: Genetic Relationships Among Chinese Bayberry Accessionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cultivar Identification and Genetic Diversity of Chinese Bayberry (Myrica rubra) Accessions Based on Fluorescent SSR Markers

et al. 2010

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“…Consequently, the SSR marker system would be useful in the construction of genetic maps. Thirteen polymorphic microsatellite loci have been developed from a genomic library in M. rubra (Terakawa et al, 2006), and eleven from a library of expressed sequence tags (ESTs) (Zhang et al, 2009a). With the development of next-generation sequencing technology, a fast and cost-effective approach to develop SSR markers from a whole genome survey has been reported (Jiao et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Development of 107 SSR markers from whole genome shotgun sequences of Chinese bayberry (Myrica rubra) and their application in seedling identification

Jia

Shen

Jiao

et al. 2014

J. Zhejiang Univ. Sci. B

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Chinese bayberry (Myrica rubra Sieb. et Zucc.) is one of the important subtropical fruit crops native to the South of China and Asian countries. In this study, 107 novel simple sequence repeat (SSR) molecular markers, a powerful tool for genetic diversity studies, cultivar identification, and linkage map construction, were developed and characterized from whole genome shotgun sequences. M13 tailing for forward primers was applied as a simple method in different situations. In total, 828 alleles across 45 accessions were detected, with an average of 8 alleles per locus. The number of effective alleles ranged from 1.22 to 10.41 with an average of 4.08. The polymorphic information content (PIC) varied from 0.13 to 0.89, with an average of 0.63. Moreover, these markers could also be amplified in their related species Myrica cerifera (syn. Morella cerifera) and Myrica adenophora. Seventy-eight SSR markers can be used to produce a genetic map of a cross between 'Biqi' and 'Dongkui'. A neighbor-joining (NJ) tree was constructed to assess the genetic relationships among accessions, and the elite accessions 'Y2010-70', 'Y2012-140', and 'Y2012-145', were characterized as potential new genotypes for cultivation.

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SSR-based genetic analysis depicted high gene diversity with subtle population genetic structure in Myrica esculenta, a high-valued edible fruit tree of the Western Himalayas

Ginwal,

Meena,

Bhandari

et al. 2024

Trees

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Development and characterization of microsatellite markers for Chinese bayberry (Myrica rubra Sieb. & Zucc.)

Cited by 14 publications

References 3 publications

Cultivar Identification and Genetic Diversity of Chinese Bayberry (Myrica rubra) Accessions Based on Fluorescent SSR Markers

Cultivar Identification and Genetic Diversity of Chinese Bayberry (Myrica rubra) Accessions Based on Fluorescent SSR Markers

Development of 107 SSR markers from whole genome shotgun sequences of Chinese bayberry (Myrica rubra) and their application in seedling identification

SSR-based genetic analysis depicted high gene diversity with subtle population genetic structure in Myrica esculenta, a high-valued edible fruit tree of the Western Himalayas

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