Development and characterization of new polyclonal antibodies specific for three polychlorinated biphenyls

Chen, Han Yu; Zhuang, Hui; Zhou, Chun

doi:10.1016/j.cclet.2008.12.022

Cited by 5 publications

(2 citation statements)

References 17 publications

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“…For obtaining hyperimmune serum, there are specific materials to be applied in the production of various immunobiological samples. Chen et al [44] developed three immunogens and obtained three pAbs to PCBs, with all of them exhibiting high antibody titers (1:32-64, agar diffusion test). Milan et al [45] developed ELISAs based on pAbs for coplanar congeners of PCBs, which showed a good stability and high specificity with the working range of 0.1-20 ppb, enough for real sample analyses.…”

Section: Antibody Productionmentioning

confidence: 99%

Antibody production and application for immunoassay development of environmental hormones: a review

Tian

Wang

Lei

et al. 2018

Chem. Biol. Technol. Agric.

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Environmental hormones, also called environmental endocrine-disrupting chemicals (EDCs), are produced by various human activities and environment pollution, and they are composed of industrial chemicals and environmental pollutants. They play a harmful role in breaking the balance of normal physiological metabolism, causing serious diseases and disrupting reproductive development in wildlife and human beings by inhibiting or imitating the action of normal gonadal hormones. Strict controls of maximum residue levels to some environmental hormones in foodstuff have been set up by many countries. Traditional detection methods, due to their high accuracy and maturity, are used extensively, such as HPLC, HPLC-MS, and GC-MS. However, these methods are time consuming and require extraordinary skills of operators, and sometimes they cannot meet the requirements of field testing. Immunoassay has the advantages of high specificity, sensitivity, simplicity, convenience, and the ability to achieve high throughput, playing an irreplaceable role in the field of rapid detection. This review focuses on the antibody production and the development of immunoassays to detect environmental hormones in food and environment. Therefore, estrogen, progesterone, and testosterone are selected as examples to introduce a series of procedures for the detection of environmental hormones including antigen synthesis, antibody production, and antibody-based detection methods.

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Section: Antibody Productionmentioning

confidence: 99%

Antibody production and application for immunoassay development of environmental hormones: a review

Tian

Wang

Lei

et al. 2018

Chem. Biol. Technol. Agric.

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“…The antiserum from the rabbit was puried by the octanoic acid-ammonium sulfate two-step precipitation method, and then introduced into SephadexA-25 and DEAE-32 columns for further purication. The puried antiserum was freeze dried, aliquoted, and stored at À20 C. 28 Antibodies were biotinylated using biotinamido-caproate-Nhydroxysuccinimide ester as previously reported. 29 Glycerin and NaN 3 were then added, and the biotinylated antibody solution was stored at À20 C.…”

Section: Preparation Of Polyclonal Antibodies and Biotinylated Polycl...mentioning

confidence: 99%

Determination of multi-residue PCBs in air by real-time fluorescent quantitative immuno-PCR assay

2014

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Amplification curves of a dilution series of Aroclor 1248 of direct competition rt-IPCR. In the figure, the fluorescence signal of the curve (10 fg mL−1) reaching the threshold was at around cycle 12.3, and there was a fall in the Ct value from 10 to 106 fg mL−1. This result implied that the time expended to reach the threshold for the high concentration of PCB molecules was much longer than for the low concentration PCBs.

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Real-time immuno-PCR assay for detecting PCBs in soil samples

Chen

Zhuang

2009

Anal Bioanal Chem

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A fast and robust assay, based on immuno-polymerase chain reaction (IPCR) techniques, was developed for the detection of polychlorinated biphenyls (PCBs) in soil samples. Real-time IPCR (rt-IPCR) is a powerful technique that combines enzyme-linked immunosorbent assay (ELISA) with the specificity and sensitivity of PCR. In our assay, indirect ELISAs based on immobilization of PCB37 hapten-ovalbumin conjugates was used for evaluation of the immune response. The effect of optimal reagent concentrations to reduce background fluorescence was also investigated. Using the optimized assay, the linear sensitivity range of the assay covered more than six orders of magnitude, and the minimum detection limits reached 5 fg ml(-1) antigen. Rt-IPCR was tested for its cross-reactivity profiles using four selected congeners and four Aroclor products. The assays were highly specific for congeners but less specific for Aroclor1242. We took four soil samples to validate the method, and the results were confirmed by gas chromatography/mass spectrometry (GC/MS). The rt-IPCR results for soil samples correlated well with the concentrations of PCBs obtained by GC/MS (r = 0.99, n = 6). These data indicate that this highly specific, sensitive, and robust assay can be modified for detecting PCB compounds in the environment.

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Development and characterization of new polyclonal antibodies specific for three polychlorinated biphenyls

Cited by 5 publications

References 17 publications

Antibody production and application for immunoassay development of environmental hormones: a review

Antibody production and application for immunoassay development of environmental hormones: a review

Determination of multi-residue PCBs in air by real-time fluorescent quantitative immuno-PCR assay

Real-time immuno-PCR assay for detecting PCBs in soil samples

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