Development and characterization of novel carrier gel core liposomes based transmission blocking malaria vaccine

Tiwari, Shivani; Goyal, Amit K.; Mishra, Neeraj; Khatri, Kapil; Vaidya, Bhuvaneshwar; Mehta, Abhinav; Wu, Yimin; Vyas, Sameer

doi:10.1016/j.jconrel.2009.08.004

Cited by 49 publications

(39 citation statements)

References 47 publications

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“…19,20 The liposomes were spun down at 20,000 × g for 30 minutes; the supernatants were diluted (if necessary), and stained with SYBR Gold dye and analyzed for siRNA content by measuring the intensity of fluorescence with an enzymelinked immunosorbent assay reader (Biochrom Ltd, Anthos 2010, Cambridge, UK) at 537 nm against a standard curve. A standard curve was established from analyzing a series of siRNA reference solutions with 200 µL measured by an enzyme-linked immunosorbent assay reader, and determined …”

Section: Entrapment Efficiencymentioning

confidence: 99%

Novel RGD-lipid conjugate-modified liposomes for enhancing siRNA delivery in human retinal pigment epithelial cells

Chen¹,

Lu²,

Yeh³

et al. 2011

IJN

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Background Human retinal pigment epithelial cells are promising target sites for small interfering RNA (siRNA) that might be used for the prevention and/or treatment of choroidal neovascularization by inhibiting the expression of angiogenic factor; for example, by downregulating expression of the vascular endothelial growth factor gene. Methods A novel functional lipid, DSPE-PEG-RGD, a Arg(R)-Gly(G)-Asp(D) motif peptide conjugated to 1, 2-distearoyl- sn -glycero-3-phosphoethanolamine- N-[maleimide (polyethylene glycol)-2000], was synthesized for the preparation of siRNA-loaded RGD-PEGylated liposomes to enhance uptake of encapsulated siRNA in retinal pigment epithelial cells. Various liposomes, with 1 mol% and 5 mol% PEGylated lipid or 1 mol% and 5 mol% RGD-PEGylated lipid, were fabricated. Results Characterization of the liposomes, including siRNA entrapment efficiency, average particle size and ζ-potential, were determined to be as follows: >96%, 129.7 ± 51 to 230.7 ± 60.7 nm, and 17.3 ± 0.6 to 32 ± 1.3 mV, respectively. For the in vitro retinal pigment epithelial cell studies, the RGD-PEGylated liposomes had high delivery efficiency with siRNA delivery, about a four-fold increase compared with the PEGylated liposomes. Comparison of the various liposomes showed that the 1 mol% RGD-modified liposome had less cytotoxicity and higher siRNA delivery efficiency than the other liposomes. The antibody blocking assay confirmed that uptake of the 1 mol% RGD-PEGylated liposome was via integrin receptor- mediated endocytosis in retinal pigment epithelial cells. Conclusion The results of this study suggest that RGD-PEGylated liposomes might be useful for siRNA delivery into retinal pigment epithelial cells by integrin receptor-medicated endocytosis.

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Section: Entrapment Efficiencymentioning

confidence: 99%

Novel RGD-lipid conjugate-modified liposomes for enhancing siRNA delivery in human retinal pigment epithelial cells

Chen¹,

Lu²,

Yeh³

et al. 2011

IJN

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“…First of all, encapsulation of hydrogel-forming components into liposomes can result from hydration of lipid cast film formed upon solvent evaporation [1,2,5,9,11,13,14,16,32], electroformation [12,22,33,34], or rapid phase evaporation [19,20]. The size of liposomes ensures the final size of lipobeads.…”

Section: Polymerization Within Liposomal Nano-/microreactorsmentioning

confidence: 99%

“…filtration [1,21], centrifugation and dialysis [5,13,20,30], or introduction of polymerization scavengers (e.g., ascorbic acid [19]) into the extravesicular space. In addition, hydrogel-forming solution as well as cross-linker and initiator can be microinjected directly into the internal compartment of a giant unilamellar phospholipid vesicle (GUV) [17].…”

Section: Liposomes 54mentioning

confidence: 99%

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Lipobeads

Казаков¹

2017

Liposomes

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A combination of lipid bilayer and cross-linked polymer network is the logical step in development of polymeric and liposomal nanoscopic systems to provide the natural level of functionality. From liposomal systems, lipobeads borrow the welldeveloped methods for preparation, diversity of lipids to control the properties of a lipid bilayer, biocompatibility of the lipid bilayer, possibility to vary size and morphology (passive targeting), availability of the external surface for attachment of various ligands (active targeting), encapsulation efficiency of both hydrophilic and hydrophobic molecules. Mechanical stability of the lipid bilayer and environmental responsiveness of the whole structure are the properties that hydrogel core brings to the new construct. The reports reviewed in this chapter demonstrate that lipobeads of nanometer and micrometer sizes can be prepared in different media, retain their stimuli responsiveness under physiological conditions, exhibit both reversible and irreversible aggregation, can be loaded with both small and high molecular weight molecules. As a platform for drug delivery systems, lipobeads have already been loaded with chemotherapeutics, malaria vaccine, and dermatological agent providing different controlled release profiles without leakage. Consecutive multistep triggering, new schemes of drug release, combined drug delivery, vesobeads, proteolipobeads, enzyme-containing lipobeads, and hemi-lipobeads are the directions for their future development.

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“…All the measurements were done in triplicate. 28,29 The entrapment efficiency of the siRNA-loaded liposomes was calculated as the ratio of siRNA used for the preparation of the original mixture as follows:…”

Section: Entrapment Efficiencymentioning

confidence: 99%

Efficient downregulation of VEGF in retinal pigment epithelial cells by integrin ligand-labeled liposome-mediated siRNA delivery

Chen¹,

Yeh²,

Shiau³

et al. 2013

IJN

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Background:The purpose of this study was to demonstrate the effectiveness of an integrin peptide ligand-labeled liposomal delivery system loaded with vascular endothelial growth factor (VEGF)-siRNA in a model study of gene therapy for retinopathy using human retinal pigment epithelial cells. Methods: Arg(R)-Gly(G)-Asp(D) motif peptide conjugating polyethylene glycol modified (RGD-PEGylated) liposomes were prepared using a thin-film hydration method and optimized for surface charge, particle size, small interfering RNA (siRNA) load, and entrapment efficiency. Reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assays were used to determine VEGF levels in retinal pigment epithelial cells. Cytotoxicity was determined using the 3-[4, 5-dimethylthiazol-2-yl]-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay and flow cytometry. Results: Physicochemical properties, including particle size, zeta potential, and siRNA load, of the prepared RGD-PEGylated liposomes and their entrapment efficiency were determined to be within the following ranges: 123.8-234.1 nm, 17.31-40.09 mV, 5.27%-6.33%, and .97%, respectively. RGD-PEGylated liposome-mediated fluorescent-labeled siRNA delivery demonstrated significantly enhanced cellular uptake, and 3 mol% RGD-PEGylated liposomes (having 3β-[N-(N', N'-dimethylaminoethane) carbamoyl] cholesterol (DC-cholesterol) DSPE and DSPE-PEG(2000)-RGD with molar ratio of 50/47/3) were shown to have better efficacy with regard to specificity for retinal pigment epithelial cells, reduced cytotoxicity, and knockdown of the target molecule. Conclusion: By integrin receptor-mediated endocytosis, 3 mol% RGD-PEGylated liposomes were shown to be a suitable vector when loaded with VEGF-siRNA for efficient downregulation of VEGF in retinal pigment epithelial cells at both the protein and gene levels. This integrin ligand-modified liposomal delivery system has therapeutic potential for ocular gene therapy.

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Development and characterization of novel carrier gel core liposomes based transmission blocking malaria vaccine

Cited by 49 publications

References 47 publications

Novel RGD-lipid conjugate-modified liposomes for enhancing siRNA delivery in human retinal pigment epithelial cells

Novel RGD-lipid conjugate-modified liposomes for enhancing siRNA delivery in human retinal pigment epithelial cells

Lipobeads

Efficient downregulation of VEGF in retinal pigment epithelial cells by integrin ligand-labeled liposome-mediated siRNA delivery

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