Development and characterization of novel cell lines from kidney and eye of rainbow trout, Oncorhynchus mykiss for virological studies

Suryakodi, S.; Majeed, Shahid; Taju, G.; Vimal, S.; Sivakumar, S.; Ahmed, A Nafeez; Shah, Fayaz Ali; Bhat, Saqib Ahmad; Sarma, Dandadhar; Begum, Ajima; Hameed, A.S. Sahul

doi:10.1016/j.aquaculture.2020.736027

Cited by 22 publications

(15 citation statements)

References 65 publications

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“…GrB cells remained stable after 1-year storage in liquid nitrogen. Their viability was similar to that from other reported fish cell lines (Ou-Yang et al, 2010;Suryakodi et al, 2021).…”

Section: Discussionsupporting

confidence: 87%

Development and characterization of a new cell line from brain tissues of the rare minnow ( Gobiocypris rarus )

Wang

et al. 2021

Aquaculture Research

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A permanent epithelial-like cell line, named GrB, was established from brain tissues of the rare minnow (Gobiocypris rarus). GrB cells grow well at temperatures between 22℃ and 27℃ in L-15 medium supplemented with 10% FBS. Chromosome analysis revealed that GrB cells possessed a diploid chromosome number of 2n = 50 at passage 50, consistent with the number of chromosomes reported for G. rarus. Partial amplification and sequence analysis of the cytochrome oxidase subunit I (COI) confirmed that GrB cells are originated from G. rarus. The cell lines also successfully expressed green fluorescence protein from plasmid pEGFP-N1 and which makes them suitable for transfection studies. A cytolytic cytopathic effect was observed in the cells after infection with tilapia lake virus 2017A (TiLV-2017A). A large number of

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“…GrB cells remained stable after 1-year storage in liquid nitrogen. Their viability was similar to that from other reported fish cell lines (Ou-Yang et al, 2010;Suryakodi et al, 2021).…”

Section: Discussionsupporting

confidence: 87%

Development and characterization of a new cell line from brain tissues of the rare minnow ( Gobiocypris rarus )

Wang

et al. 2021

Aquaculture Research

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“…In cell culture research, mycoplasma contamination is a serious problem since it affects cell growth, metabolic rate and chromosome number. In the present study, Hoechst 33258 stain was used to detect mycoplasma as reported by several investigators (Freiberg & Masover, 1990; Majeed et al., 2013; Suryakodi et al., 2021). No extranuclear fluorescence was observed in the established cell lines of snubnose pompano.…”

Section: Discussionmentioning

confidence: 93%

“…In the present study, the plating efficiency at the cell concentration of 1000 per flask was estimated to be about 64.22%, 53.46%, 61.24%, 58.23% and 68.42%, for the cell lines of SPG, SPH, SPK, SPE and SPF, respectively, whereas the plating efficiency was found to be less in all the cell lines at the seeding density of 100 cells per flask. High plating efficiency was observed in gill and fin cell lines of snubnose pompano as observed in kidney cell line of rainbow trout (Suryakodi et al, 2021), caudal fin cell line of T. tor (Yadav et al, 2012), SAF-1 cell line (Bejar et al, 1997) and GF-1 cells (Chi et al, 1999). an important factor since its use for various applications, especially gene expression studies to produce recombinant proteins, depends on it.…”

Section: Replication Efficiency Of Fnv and Tilvmentioning

confidence: 99%

“…In India, fish cell culture research was initiated in 1995 (Sathe et al, 1995) and the first continuous cell line from the kidney of seabass, Lates calcarifer, was developed in 2006 (Sahul Hameed et al, 2006). Since 2006, cell lines from a variety of fish belonging to different habitats have been developed for various applications (Ishaq Ahmed et al, 2008;Lakra et al, 2010;Majeed et al, 2015;Nanthini et al, 2019;Parameswaran et al, 2007;Sarath Babu et al, 2012;Sobhana et al, 2009;Sood et al, 2015;Suryakodi et al, 2021;Swaminathan et al, 2015). A repository has been established to maintain 63 fish cell lines developed from different fish species for use by researchers (www.aahl.res.in).…”

mentioning

confidence: 99%

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Development and characterization of five novel cell lines from snubnose pompano, Trachinotus blochii (Lacepede, 1801), and their application in gene expression and virological studies

Ahmed

Seepoo

Taju

et al. 2021

Journal of Fish Diseases

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In India, intense interest has been generated in the last three decades to develop mariculture in coastal waters. Recently, Indian mariculture has turned its attention to finfish culture from penaeid shrimp because of serious viral diseases, which resulted in severe economic loss in the shrimp culture industry every year. Snubnose pompano (Trachinotus blochii) is a pelagic marine fish and is considered to be a fish with high market value. Its current price is approximately $ 4 per kg at fish landing centres in India (Jayakumar

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“…Appropriate amounts of the 30th passage SCC cells were harvested by trypsin digestion and the genomic DNA was extracted using the Cytogenome Extraction Kit (Tiangen, China) according to the manufacturer's instruction. Two pairs of primers were used to amplify the 16S rRNA and COI gene fragments of S. chuatsi (Palumbi et al, 1996; Suryakodi et al, 2021; Table 1). The PCR reaction mixture (25 μl) contained 12.5 μl of 2× Flash Hot Start MasterMix (Dye) (CoWin Biosciences, China), 1 μl each of primer pair (10 μmol/L), 1.5 μl of template DNA and 9 μl of sterile distilled H 2 O.…”

Section: Methodsmentioning

confidence: 99%

Establishment and characterization of a spinal cord tissue cell line from mandarin fish, Siniperca chuatsi and its susceptibility to several viruses

Gong

Lin

et al. 2022

Journal of Fish Diseases

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Siniperca chuatsi, a mandarin fish, is a unique species of freshwater fish in China. It is one of the most economically important freshwater fish due to its delicious taste and high nutritional value. In 2019, the total farmed production of S. chuatsi in China was nearly 320,000 tons, with an output value exceeding RMB 20 billion (Li et al., 2021). Mandarin fish are mainly farmed in Guangdong, Hubei, Jiangxi, Anhui and Jiangsu Provinces of China, with the highest production in Guangdong. The expansion of the scale and density of mandarin fish farming has led to a variety of pathogenic microorganisms causing disease outbreaks in mandarin fish (Sun et al., 2017). A number of bacterial, parasitic and viral diseases have been reported from the mandarin fish. Among the viruses causing infectious diseases in fish, the highly infectious and lethal viruses Infectious Spleen and Kidney Necrosis Virus (ISKNV), Siniperca chuatsi ranavirus (SCRaV) and Siniperca chuatsi rhabdovirus (SCRV), have been reported in the mandarin fish and cause considerable losses to mandarin fish aquaculture. For example, SCRV outbreaks in hundreds of fish farms in Guangdong were reported, which caused death of more than 10 million juvenile mandarin fish (Zhang et al., 2020). Therefore, the prevention and treatment of these viral diseases has become an urgent problem to be solved in the mandarin fish aquaculture.

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Development and characterization of novel cell lines from kidney and eye of rainbow trout, Oncorhynchus mykiss for virological studies

Cited by 22 publications

References 65 publications

Development and characterization of a new cell line from brain tissues of the rare minnow ( Gobiocypris rarus )

Development and characterization of a new cell line from brain tissues of the rare minnow ( Gobiocypris rarus )

Development and characterization of five novel cell lines from snubnose pompano, Trachinotus blochii (Lacepede, 1801), and their application in gene expression and virological studies

Establishment and characterization of a spinal cord tissue cell line from mandarin fish, Siniperca chuatsi and its susceptibility to several viruses

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