Development and characterization of portable smartphone‐based imaging device

Banik, Soumyabrata; Mahato, Krishna Kishore; Antonini, Andrea; Mazumder, Nirmal

doi:10.1002/jemt.23525

Cited by 15 publications

(12 citation statements)

References 33 publications

(46 reference statements)

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“…Accordingly, products have been developed to mount smartphones onto microscope eyepieces for image acquisition. In addition, standalone 'smartphone microscope' designs have been developed for use in K-12 and undergraduate science education settings 1-6 , research or clinical settings [7][8][9][10][11] , and geoscience applications 12 .Fluorescence microscopy produces visually attractive images that can greatly enhance the contrast of specific features of the specimen being viewed. This microscopic imaging modality has historically involved costly and cumbersome mercury arc lamps or lasers.…”

mentioning

confidence: 99%

A low-cost smartphone fluorescence microscope for research, life science education, and STEM outreach

Schaefer

Nelson

Butrum

et al. 2023

Sci Rep

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Much of our understanding of cell and tissue development, structure, and function stems from fluorescence microscopy. The acquisition of colorful and glowing images engages and excites users ranging from seasoned microscopists to STEM students. Fluorescence microscopes range in cost from several thousand to several hundred thousand US dollars. Therefore, the use of fluorescence microscopy is typically limited to well-funded institutions and biotechnology companies, research core facilities, and medical laboratories, but is financially impractical at many universities and colleges, primary and secondary schools (K-12), and in science outreach settings. In this study, we developed and characterized components that when used in combination with a smartphone or tablet, perform fluorescence microscopy at a cost of less than $50 US dollars per unit. We re-purposed recreational LED flashlights and theater stage lighting filters to enable viewing of green and red fluorophores including EGFP, DsRed, mRFP, and mCherry on a simple-to-build frame made of wood and plexiglass. These devices, which we refer to as glowscopes, were capable of 10 µm resolution, imaging fluorescence in live specimens, and were compatible with all smartphone and tablet models we tested. In comparison to scientific-grade fluorescence microscopes, glowscopes may have limitations to sensitivity needed to detect dim fluorescence and the inability to resolve subcellular structures. We demonstrate capability of viewing fluorescence within zebrafish embryos, including heart rate, rhythmicity, and regional anatomy of the central nervous system. Due to the low cost of individual glowscope units, we anticipate this device can help to equip K-12, undergraduate, and science outreach classrooms with fleets of fluorescence microscopes that can engage students with hands-on learning activities.

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mentioning

confidence: 99%

A low-cost smartphone fluorescence microscope for research, life science education, and STEM outreach

Schaefer

Nelson

Butrum

et al. 2023

Sci Rep

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“…In total, six to nine main spikes of each accession (two to three from each plot) were randomly selected from the middle of the plots for phenotyping. These were dissected manually starting from the stem elongation stage (Alqudah & Schnurbusch, 2014) to measure and count the MYP, DS and UDS at GA and TIP developmental stages using portable USB Digital Microscope Magnification (Banik et al, 2020; Rather et al, 2019). Each genotype was dissected three to four times between the stem elongation stage and the heading stage (HD) to define MYP, GA and TIP developmental stages precisely.…”

Section: Methodsmentioning

confidence: 99%

Insight into the genetic contribution of maximum yield potential, spikelet development and abortion in barley

Alqudah

Sharma

Börner

2021

Plants People Planet

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Societal Impact Statement To feed the world's ever‐increasing population, new genetic approaches are required. Increasing the number of living spikelets is one promising way to improve grain yield. This, in turn, increases the number of spikelets per plant, thereby increasing the total yield. We present the first evidence for genetic control of alive spikelets in barley. Discovering natural variation as well as genomic regions associated with these traits will serve as a benchmark in future breeding for improving grain yield. Summary The primary goal of most breeding programmes is to increase grain yield. However, one of the many methods for raising yield that is yet to be fully investigated is increasing the number of spikelets by minimising spikelet abortion. Spikelet abortion dramatically increases during the late reproductive phase, but the molecular and genetic mechanisms remain unknown. Here, we employed a phenotyping approach in which developed and undeveloped spikelets were detected and counted during spike development and their maximum yield potential (MYP) was investigated. We studied 20 agronomic and spikelet‐related traits using a set of 184 diverse spring barley accessions under field conditions. By employing a set of >125K SNPs, GWAS was conducted. Our analysis revealed 26 genetic clusters associated with MYP and the number of developed and undeveloped spikelets. Most of the significant associated genomic regions were co‐located near the candidate genes of phytohormones such as ABA, auxin, and cytokinin suggesting the importance of phytohormones in keeping spikelets alive, their development, and MYP. Our findings point to a potential link between jasmonic acid and the MYP, development and abortion of spikelets. We further provide genetic evidence that sugar‐related genes and sucrose have the potential to regulate MYP, spikelet development and spikelet survival. Our findings can be used for marker‐assisted breeding and as a resource for future molecular and genetic validation. Collectively, we propose a new genetic network linking spikelet‐related traits to grain yield determinants.

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Section: Introductionmentioning

confidence: 99%

A low-cost smartphone fluorescence microscope for research, life science education, and STEM outreach

Schaefer

Nelson

Butrum

et al. 2022

Preprint

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Much of our understanding of cell and tissue development, structure, and function stems from fluorescence microscopy. The acquisition of colorful and glowing images engages and excites users ranging from seasoned microscopists to STEM students. Fluorescence microscopes range in cost from several thousand to several hundred thousand US dollars. Therefore, the use of fluorescence microscopy is typically limited to well-funded institutions and biotechnology companies, research core facilities, and medical laboratories, but is financially impractical at many universities and colleges, primary and secondary schools (K-12), and in science outreach settings. In this study, we developed and characterized components that when used in combination with a smartphone or tablet, perform fluorescence microscopy at a cost of less than $50 US dollars per unit. We re-purposed recreational LED flashlights and theater stage lighting filters to enable viewing of green and red fluorophores including EGFP, DsRed, mRFP, and mCherry on a simple-to-build frame made of wood and plexiglass. These devices, which we refer to as glowscopes, were capable of 10 micron resolution, imaging fluorescence in live specimens, and were compatible with all smartphone and tablet models we tested. In comparison to scientific-grade fluorescence microscopes, glowscopes may have limitations to sensitivity needed to detect dim fluorescence and the inability to resolve subcellular structures. We demonstrate capability of viewing fluorescence within zebrafish embryos, including heart rate, rhythmicity, and regional anatomy of the central nervous system. Due to the low cost of individual glowscope units, we anticipate this device can help to equip K-12, undergraduate, and science outreach classrooms with fleets of fluorescence microscopes that can engage students with hands-on learning activities.

show abstract

Development and characterization of portable smartphone‐based imaging device

Cited by 15 publications

References 33 publications

A low-cost smartphone fluorescence microscope for research, life science education, and STEM outreach

A low-cost smartphone fluorescence microscope for research, life science education, and STEM outreach

Insight into the genetic contribution of maximum yield potential, spikelet development and abortion in barley

A low-cost smartphone fluorescence microscope for research, life science education, and STEM outreach

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