Development and efficacy of an attenuated Vibrio harveyi vaccine candidate with cross protectivity against Vibrio alginolyticus

Hu, Yonghua; Deng, Tian; Sun, Bo; Sun, Li

doi:10.1016/j.fsi.2012.03.032

Cited by 24 publications

(19 citation statements)

References 43 publications

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“…Higher RPS generally means better immune protective effect (Yun et al 2010;Hu et al 2012;Mohammed et al 2013). In our study, the RPS of FKC vs BSA and OMP vs BSA group challenged by V. vulnificus was 60 and 80%, respectively (Fig.…”

Section: Figuresupporting

confidence: 53%

Immunogenicity study of an expressed outer membrane protein U ofVibrio vulnificusin Japanese eel (Anguilla japonica)

Duan

Feng

et al. 2018

J Appl Microbiol

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Section: Figuresupporting

confidence: 53%

Immunogenicity study of an expressed outer membrane protein U ofVibrio vulnificusin Japanese eel (Anguilla japonica)

Duan

Feng

et al. 2018

J Appl Microbiol

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“…The amount of serum antibody (IgM) specific to V. harveyi was measured by ELISA as described previously (Hu, Deng, Sun, & Sun, ). Briefly, 96‐well ELISA plates were coated with 10 7 colony‐forming units (CFUs) of formalin‐killed V. harveyi cells per well (100 μl) at 4°C overnight and subsequently blocked with 5% skimmed milk in PBS containing 0.05% Tween‐20 (PBST).…”

Section: Methodsmentioning

confidence: 99%

“…The amount of serum antibody (IgM) specific to V. harveyi was measured by ELISA as described previously (Hu, Deng, Sun, & Sun, 2012…”

Section: Antibody Measurementmentioning

confidence: 99%

Enhanced immune responses and effectiveness of refined outer membrane protein vaccines against Vibrio harveyi in orange‐spotted grouper (Epinephelus coioides)

Nguyen

Chen

et al. 2018

Journal of Fish Diseases

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Vibriosis is a severe infection occurring in many commercially important marine fish species. In this study, vaccines containing Vibrio harveyi recombinant outer membrane protein K (rOmpK), outer membrane protein U (rOmpU) and rOmpK-OmpU fusion protein in addition to the metabolizable Montanide ISA 763 A VG adjuvant were developed and evaluated in the orange-spotted grouper. The results indicate that recombinant V. harveyi protein-based vaccines resulted in a remarkably higher expression of IL-1β and IL-8 at 24 hr, and greater antibody production, as early as 2 weeks postimmunization. Notably, enhanced immune responses and significant protective efficacy against V. harveyi infections were observed in the fusion protein vaccine-injected fishes with relative per cent survival value of 81.8%. Additionally, the rOmpK-OmpU antisera presented a high bactericidal effect on not only V. harveyi, but also Vibrio parahaermolyticus and Vibrio alginolyticus. Our results demonstrated that the fusion protein rOmpK-OmpU was an effective vaccine candidate that exhibited potentially great versatility for controlling vibrio infections.

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“…We found that compared to the wild type, C312M was moderately retarded in growth when cultured in the rich LB medium and drastically retarded when cultured in the presence of the iron chelator 2, 2'-dipyridyl, suggesting that C312M was impaired in growth, particularly under conditions of iron depletion. For pathogenic bacteria, mutation to an antibiotic-resistant phenotype is sometimes associated with alteration in protein expression (Zhang et al 2006, LaFrentz et al 2008, Hu et al 2012. In the present study, we found that the whole-cell protein profile of C312M differed from that of C312 in several places.…”

Section: Discussionmentioning

confidence: 47%

C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine

Yu¹,

Hu²,

Sun³

et al. 2012

Dis. Aquat. Org.

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Vibrio anguillarum, a Gram-negative bacterial pathogen, is the causative agent of vibriosis that affects a wide range of aquatic animals. In this study, we obtained a mutant V. anguillarum, C312M, derived from the pathogenic V. anguillarum C312 by selection of rifampicin resistance. C312M was slower in growth than the wild type C312, particularly under conditions of iron depletion. Compared to C312, C312M was altered in protein production profile and exhibited a dramatically increased median lethal dose. Safety analysis showed that C312M was stable in virulence in the absence of selective pressure. To examine the potential of C312M as a live attenuated vaccine, Japanese flounder Paralichthys olivaceus were vaccinated with C312M via oral, immersion, and oral plus immersion routes. Microbiological analysis showed that C312M was recovered from the gut, liver, kidney, and spleen of the vaccinated fish in 1 to 14 d post-vaccination. When the fish were challenged with C312 at 1 mo post-vaccination, C312M-vaccinated fish exhibited relative percent survival rates of 60 to 84%. Comparable protection was observed when the fish were challenged with a heterologous V. anguillarum strain. Further analysis showed that C312M-vaccinated fish produced specific serum antibodies which enhanced serum bactericidal activity in a manner that is probably complement-dependent. These results indicate that C312M is highly attenuated in virulence but still retains residual infectivity, and that C312M is an effective vaccine when delivered alive via immersion and oral feeding.

show abstract

Development and efficacy of an attenuated Vibrio harveyi vaccine candidate with cross protectivity against Vibrio alginolyticus

Cited by 24 publications

References 43 publications

Immunogenicity study of an expressed outer membrane protein U ofVibrio vulnificusin Japanese eel (Anguilla japonica)

Immunogenicity study of an expressed outer membrane protein U ofVibrio vulnificusin Japanese eel (Anguilla japonica)

Enhanced immune responses and effectiveness of refined outer membrane protein vaccines against Vibrio harveyi in orange‐spotted grouper (Epinephelus coioides)

C312M: an attenuated Vibrio anguillarum strain that induces immunoprotection as an oral and immersion vaccine

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