2024
DOI: 10.5812/jjm-144579
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Development and Evaluation of a Multiplex Fluorescence PCR for Salmonella Virulence Genes Analysis

Peiyan He,
Yong Yan,
Guoying Zhu
et al.

Abstract: Background: Salmonellosis, a disease caused by Salmonella, is a significant public health concern and economic burden worldwide. The ability of various Salmonella serovars to cause disease is closely linked to the virulence genes they possess. Objectives: The aim of this study was to develop a multiplex fluorescence PCR for detecting ten major virulence genes (ssaR, spvC, pefA, sipA, fimA, sifA, sopE2, sopB, prgH, and stn) in Salmonella. Methods: Primer pairs specific to ten target virulence genes were designe… Show more

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