The rail irregularities and wheel-rail interactions in a train running at high speeds may result in large-amplitude vibrations in the train's car body and affect passengers by reducing ride comfort. The train suspension systems have a crucial role in reducing the vibrations and improving ride comfort to an acceptable level. In this context, an exclusive magneto-rheological (MR) damper with a favorable dynamic range was designed and fabricated. The MR dampers were installed in a high-speed train's secondary lateral suspension system, replacing passive hydraulic dampers to mitigate vibration of the car body and keep the ride comfort level in a proper condition. A unique full-scale experimental investigation on the high-speed train equipped with MR dampers was carried out to evaluate the MR damper functionality in a real operating situation. The full-scale roller experiments were conducted in a vast range of speeds from 80 to 350 km/hr. At each speed, different currents were applied to the MR dampers. The car body dynamic responses were collected with accelerometers and displacement sensors mounted on the carriage floor and sidewall. To investigate the ride quality of the high-speed train, ride comfort indices and car body rolling motion are evaluated. Ride comfort indices under various train operating conditions are calculated through Sperling and UIC513 rules. This study reveals that the designed MR dampers effectively reduce the car body's rolling motion. According to Sperling ride comfort index, the car body vibration was "clearly noticeable" at some running speeds when adopting the MR dampers, but it was not unpleasant. Besides, a "very good comfort" was achieved according to the UIC513 ride comfort criterion. Also, no train instability was whatsoever observed at high speeds.INDEX TERMS High-speed train, MR damper, full-scale experiment, ride comfort index, rolling motion, secondary suspension system.