Development and evaluation of a novel loop-mediated isothermal amplification (LAMP) method targeting Theileria parasites infecting Yezo sika deer

Shirozu, Takahiro; Badolo, Athanase; Soga, Akira; Yoshimura, Aya; Morishita, Yu ki; Koketsu, Mamoru; Shirafuji, Rika Umemiya; Inokuma, Hisashi; Yokoyama, Naoki; Fukumoto, Shinya

doi:10.1016/j.parint.2020.102130

Cited by 4 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition to the aforementioned techniques, there are many alternatives that hold the potential of POCT-based molecular diagnosis applications. The exponential amplification reaction (EXPAR) and signal-mediated amplification of RNA technology (SMART), which fall under the umbrella of isothermal NAAT, can offer the possibility for POCT-based molecular diagnosis and have been used for different desires [134] , [135] . Besides, Yin et al utilized a cascade enzymatic polymerization reaction to detect and quantify microRNA by employing DNA polymerase with the isothermal strand-displacement property and restriction endonuclease with nicking activity [136 ] .…”

Section: Available Techniques For Poc Molecular Diagnosismentioning

confidence: 99%

Recent advances and challenges of biosensing in point-of-care molecular diagnosis

Chu

Liu

Liu³

et al. 2021

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

Molecular diagnosis, which plays a major role in infectious disease screening with successful understanding of the human genome, has attracted more attention because of the outbreak of COVID-19 recently. Since point-of-care testing (POCT) can expand the application of molecular diagnosis with the benefit of rapid reply, low cost, and working in decentralized environments, many researchers and commercial institutions have dedicated tremendous effort and enthusiasm to POCT-based biosensing for molecular diagnosis. In this review, we firstly summarize the state-of-the-art techniques and the construction of biosensing systems for POC molecular diagnosis. Then, the application scenarios of POCT-based biosensing for molecular diagnosis were also reviewed. Finally, several challenges and perspectives of POC biosensing for molecular diagnosis are discussed. This review is expected to help researchers deepen comprehension and make progresses in POCT-based biosensing field for molecular diagnosis applications.

show abstract

Section: Available Techniques For Poc Molecular Diagnosismentioning

confidence: 99%