Development and evaluation of a competitive ELISA using a monoclonal antibody for antibody detection after goose parvovirus virus-like particles (VLPs) and vaccine immunization in goose sera

Wang, Qin; Ju, Huanyu; Li, Yanwei; Jing, Zhiqiang; Guo, Lu; Zhao, Yu; Ma, Bo; Gao, Mingchun; Zhang, Wenlong; Wang, Junwei

doi:10.1016/j.jviromet.2014.08.021

Cited by 11 publications

(11 citation statements)

References 11 publications

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“…The cut-off value was determined as the mean S/P value of the 33 negative samples +3 standard deviations. This interval represents 99.6% of the negative samples [ 26 ]. To evaluate the impact of heat-inactivation of serum (30 min at 56 °C) on SBV-N specific IgM detectability by ELISA, a selection of samples was tested in the IgM ELISA described above without (IgM ELISAw/d) and with (IgM ELISAd) heat-inactivation of the complement system.…”

Section: Methodsmentioning

confidence: 99%

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Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep

Poskin

Verite²,

Comtet³

et al. 2015

Vet Res

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Schmallenberg virus (SBV) is an Orthobunyavirus that induces abortion, stillbirths and congenital malformations in ruminants. SBV infection induces a long lasting seroconversion under natural conditions. The persistence of the protective immunity and the isotype specific antibody response upon SBV infection of sheep has however not been studied in detail. Five sheep were kept in BSL3 facilities for more than 16 months and subjected to repeated SBV infections. Blood was regularly sampled and organs were collected at euthanasia. The presence of SBV RNA in serum and organs was measured with quantitative real-time PCR. The appearance and persistence of neutralizing and SBV nucleoprotein (N) isotype specific antibodies was determined with virus neutralization tests (VNT) and ELISAs. The primo SBV infection protected ewes against clinical signs, viraemia and virus replication in organs upon challenge infections more than 15 months later. Production of neutralizing SBV specific antibodies was first detected around 6 days post primo-inoculation with VNT and correlated with the appearance of SBV-N specific IgM antibodies. These IgM antibodies remained present for 2 weeks. SBV-N specific IgG antibodies were first detected between 10 and 21 dpi and reached a plateau at 28 dpi. This plateau remained consistently high and no significant decrease in titre was found over a period of more than 1 year. Similar results were found for the neutralising antibody response. In conclusion, the SBV specific IgM response probably eliminates SBV from the blood and the protective immunity induced by SBV infection protects sheep against reinfection for at least 16 months.

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Section: Methodsmentioning

confidence: 99%

“…The cut-off value was determined as the mean S/P value of the 50 samples ± 3 × standard deviation. This interval represents 99.6% of the negative samples [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep

Poskin

Verite²,

Comtet³

et al. 2015

Vet Res

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“…It suggests that the epitope detected by this mAb is highly specific to the newly defined spiroplasma, just like the first mAb 6H7 we produced. To date, many specific mAbs have been used in the establishment of immunological assays for the detection of pathogenic microbial antigens 12 13 14 . These 2 mAbs can therefore be used to distinguish S. eriocheiris from other spiroplasmas.…”

Section: Discussionmentioning

confidence: 99%

Production and application of polyclonal and monoclonal antibodies against Spiroplasma eriocheiris

Zhang

Bao

Miao

et al. 2015

Sci Rep

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A new species of spiroplasma, Spiroplasma eriocheiris (S. eriocheiris), was identified as a lethal pathogen of tremor disease (TD) in Chinese mitten crab recently. In order to acquire appropriate biological and diagnostic tools for characterizing this newly discovered pathogen, 5 monoclonal antibodies (mAbs) and a polyclonal antibody (pAb) against S. eriocheiris were produced. Among the mAbs, 6F5, 7C8 and 12H5 lead to the deformation of S. eriocheiris. A peptide sequence, YMRDMQSGLPRY was identified as a mimic motif of MreB that is the cell shape determining protein of S. eriocheiris interacting with 3 mAbs. Furthermore, a double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) for detection of S. eriocheiris was established using the mAb and pAb we prepared. It detected as low as 0.1 μg/mL of S. eriocheiris. No cross-reaction was observed with three other common bacteria (Pseudomonas aeruginosa, Escherichia coli, and Bacillus subtilis) and the hemolymph samples of healthy Eriocheir sinensis. Collectively, our results indicated that the mAbs and pAb we prepared could be used in the analysis of S. eriocheiris membrane proteins mimotope and development of a diagnostic kit for S. eriocheiris infections.

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“…Rapid serological antibody test of pathogen infection is important for on-site initial screening, 1,2 epidemiological surveys 3 and the origin of new epidemic diseases. 4,5 In particular, a quantification test for target antibody can determine the protective power of vaccines 6,7 and determine whether the infected host is in active or recovery phase. 8 Methods for antibody test of particular epitope, using monoclonal antibody, are necessary in many cases, including distinguishing infections due to pathogens of different subtypes with similar genomes, 9 differential diagnosis of naturally infected versus vaccinated hosts 10,11 and elimination of cross-reactivity.…”

Section: Introductionmentioning

confidence: 99%

Rapid Quantum Dot Nanobead-mAb Probe-Based Immunochromatographic Assay for Antibody Monitoring of Trichinella spiralis Infection

Xu¹,

Liu²,

Li³

et al. 2021

IJN

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Purpose: Sensitive and selective point-of-care biosensor is an urgent pursuit of serological antibody detection to control parasite pathogen. For specific, quantitative and on-site screening of Trichinella spiralis infection in livestock, a quantum dot nanobead-monoclonal antibody (QB-mAb) probe-based immunochromatographic assay (ICA) was developed by introducing a competitive sandwich strategy (QB-CICA). Methods: In the QB-CICA, QB-mAb probes competed with serum antibody for a particular epitope, followed by immunocomplexes binding to capture antibody on the test line. With the accumulation of target antibody, captured probes served as signal elements for fluorescent readout in a "turn off" mode, along with the fluorescence gradually weakened. The sensitivity and standard calibration curve of the QB-CICA were quantified using swine sera as negative control (n = 200) and artificial infected swine sera (n = 80) compared with a commercial ELISA kit. Besides, Trichinella spiralis-antibody targeting test ability of the QB-CICA, instead of other parasites or viruses antibodies (n = 10), was evaluated. Results: The QB-CICA exhibited a good linear range, a low detection limit of 189.92 ng mL −1 and 100% selectivity that was higher than commercial ELISA kit (90%), as well as the same serological positive rate (100%) with commercial ELISA kit in different infection dose models. Conclusion:Taking advantage of its simplicity, short response time (25 min), sensitivity and specificity, the proposed QB-CICA has potential applications for parasite-related antibody monitoring in food safety and clinical diagnosis fields.

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Development and evaluation of a competitive ELISA using a monoclonal antibody for antibody detection after goose parvovirus virus-like particles (VLPs) and vaccine immunization in goose sera

Cited by 11 publications

References 11 publications

Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep

Persistence of the protective immunity and kinetics of the isotype specific antibody response against the viral nucleocapsid protein after experimental Schmallenberg virus infection of sheep

Production and application of polyclonal and monoclonal antibodies against Spiroplasma eriocheiris

Rapid Quantum Dot Nanobead-mAb Probe-Based Immunochromatographic Assay for Antibody Monitoring of Trichinella spiralis Infection

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