Development and Evaluation of a Novel Loop-Mediated Isothermal Amplification Method for Rapid Detection of Severe Acute Respiratory Syndrome Coronavirus

Detection methods for milk pathogenic bacteria by loop-mediated isothermal amplification

“…The present study demonstrated the minimum time for formation of electrophoresis strips at 60 min. This was similar to earlier studies (21,22). Following the standardization of temperature and time, optimization of the LAMP assay was also carried out with regard to the effect of the amount of primers and Bst DNA polymerase large fragment on formation of electrophoresis strips on agrose.…”

Development of a reverse transcription loop-mediated isothermal amplification assay for visual detection of avian reovirus

“…The viral RNA can be detected by one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay performed with reverse transcriptase and LAMP reaction mixture. Currently, the assay is being widely used for the detection of various pathogens including viruses, bacteria and parasites (Thai et al, 2004;Pham et al, 2005;Poon et al, 2005;Curtis et al, 2008;Rovira et al, 2009;Xu et al, 2009;Yamazaki et al, 2009).…”

Development and Evaluation of a Real-Time Reverse Transcription-Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Rift Valley Fever Virus in Clinical Specimens

“…The loop-mediated isothermal amplification (LAMP) method has been shown to be highly accurate for the detection of DNA (4,20) and RNA (14,32,36) viruses, differentiation of viral serotypes and subtypes (17,21), and rapid diagnosis of bacterial infections (5). LAMP amplifies target nucleic acid under isother-mal conditions, usually between 60°C and 65°C (19).…”