Development and evaluation of a rapid neutralizing antibody test for rabies

Shiota, Seiji; Mannen, Kazuaki; Matsumoto, Takashi; Yamada, Kentaro; Yasui, Takehito; Takayama, Katsuyoshi; Kobayashi, Yuki; Khawplod, Pakamatz; Gotoh, Kazuyo; Ahmed, Kamruddin; Iha, Hidekatsu; Nishizono, Akira

doi:10.1016/j.jviromet.2009.05.018

Cited by 32 publications

(22 citation statements)

References 13 publications

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“…Consistent with our previous studies (Shiota et al., ) (Nishizono et al., ), we found significant concordance between the RAPINA and RFFIT results in the Philippines, Thailand and Japan. In terms of the negative predictive value, there remains room for improvement.…”

Section: Discussionsupporting

confidence: 93%

“…Recently, several alternatives to the RFFIT and FAVN test have been developed to estimate VNA titres (Shiota et al., ) (Ma et al., ) (Wang et al., ) (Batista et al., ) (Madhusudana et al., ). As one of the alternative methods, we recently developed the RAPINA, which is an easy, rapid and inexpensive immunochromatography‐based competitive test using formalin‐fixed virus particles for the detection of RABV G protein‐specific antibodies (Shiota et al., ) (Nishizono et al., ) (Nishizono, ). To determine the performance of the test in comparison with RFFIT in different settings, the kit was distributed to rabies laboratories in Japan, Thailand and Sri Lanka for preliminary evaluation.…”

Section: Discussionmentioning

confidence: 99%

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A Comparative Study of the RAPINA and the Virus‐Neutralizing Test (RFFIT) for the Estimation of Antirabies‐Neutralizing Antibody Levels in Dog Samples

Manalo

Yamada

Watanabe

et al. 2016

Zoonoses and Public Health

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Impacts• The mass vaccination of dogs against rabies is the most rational strategy for interrupting the natural transmission of rabies. Knowledge of the virusneutralizing antibody (VNA) level against the rabies virus (RABV) is required to evaluate herd immunity of dogs in mass vaccination campaigns.• The rapid rabies antibody test (RAPINA) is a novel, immunochromatographic test that uses inactivated virus to estimate the VNA level qualitatively and is a simple, rapid and inexpensive test. We report a comparative study of the RAPINA and the virus-neutralizing test (RFFIT) for the estimation of antirabies-neutralizing antibody level in dog sample.• Comparing the performance of the two tests for evaluating the vaccination status of dogs in the Philippines, Thailand and Japan, the positive and negative predictive values and concordance of the RAPINA were highly homologous and reproducible among different laboratories. The RAPINA is appropriate to survey vaccination coverage in countries with limited resources. SummaryThe mass vaccination of dogs against rabies is a highly rational strategy for interrupting the natural transmission of urban rabies. According to the World Organization for Animal Health (OIE) and the World Health Organization (WHO), the immunization of at least 70% of the total dog population minimizes the risk of endemic rabies. Knowledge of the virus-neutralizing antibody (VNA) level against the rabies virus (RABV) is required to evaluate protective immunity and vaccine coverage of dogs in the field. The rapid focus fluorescent inhibition test (RFFIT) and the fluorescent antibody virus neutralization (FAVN) test are recommended by OIE and WHO to determine the VNA levels in serum. However, these tests are cell culture based and require the use of live viruses and specialized equipment. The rapid neutralizing antibody test (RAPINA) is a novel, immunochromatographic test that uses inactivated virus to estimate the VNA level qualitatively. It is a simple, rapid and inexpensive, although indirect, assay for the detection of VNA levels. The RAPINA has shown good positive and negative predictive values and a high concordance with the RFFIT results. In this study, we compared the performance of the two tests for evaluating the vaccination status of dogs in the Philippines, Thailand and Japan. A total of 1135 dog 355This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.Zoonoses and Public Health sera were analysed by the RAPINA and compared to the VNA levels determined by the RFFIT. The overall positive and negative predictive values of the RAPINA were 96.2-99.3% and 84.5-94.8%, respectively, with a concordance (kappa) of 0.946-0.97 among the three countries. The RAPINA results were highly homologous and reproducible among different laboratories. These results suggest that this test ...

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

A Comparative Study of the RAPINA and the Virus‐Neutralizing Test (RFFIT) for the Estimation of Antirabies‐Neutralizing Antibody Levels in Dog Samples

Manalo

Yamada

Watanabe

et al. 2016

Zoonoses and Public Health

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“…However, the methods used to measure the rabies VNA level, such as virus neutralization assays and ELISA, are inconvenient and require specialized laboratories and equipment, and trained personnel. Recently, we developed a rapid, safe, easy, and inexpensive VNA test for rabies based on a combination of an immunochromatographic technique and a competitive immunoassay with neutralizing monoclonal antibodies (14,15). This semiquantitative test determines within 60 min whether a dog has protective VNA levels, without using a live virus and expensive equipment.…”

Section: Discussionmentioning

confidence: 99%

Relationship between Virus-Neutralizing Antibody Levels and the Number of Rabies Vaccinations: a Prospective Study of Dogs in Japan

et al. 2013

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SUMMARY:A mass rabies vaccination of dogs has been conducted annually in Japan over the last 60 years. To assess both current levels of rabies virus-neutralizing antibody (VNA) in dogs and the rationale for current vaccination procedures, we used a rapid fluorescent focus inhibition test to determine VNA levels in 756 dogs that had visited animal hospitals in Japan. We found that 51.1z of the dogs that had received 1 rabies vaccination had protective VNA levels (AE0.5 IU/ml) with a geometric mean of 0.61 IU/ml. In contrast, 97.8z of the dogs that had been vaccinated at least twice had protective VNA levels with a geometric mean of 7.86 IU/ml. Furthermore, 97.9-100z of the dogs vaccinated at least twice retained protective VNA levels into the second year after the last vaccination. Although VNA levels in the dogs vaccinated at least twice tended to decline 2 years after the last vaccination, 78.9z retained protective VNA levels. Thus, the current rabies vaccination schedule provides adequate protection, but the registration system and vaccination schedule needs to be improved to ensure that increased numbers of dogs are vaccinated against rabies.

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“…The sensitivity, specificity, and accuracy of the prepared SE-Lateral flow kits reported were 94.4%, 90%, and 94%, respectively (Ibrahim et al, 2017). Furthermore, Shiota et al (2009) developed a lateral flow test for the detection of antibodies against the rabies virus and reported the sensitivity and specificity of 88.7% and 91.9%, respectively, as compared with ELISA. The prepared LFD did not have the ability to differentiate between the infected and vaccinated animals; the same gave results positive at IgG test line 1.…”

Section: Results a N D Discussionmentioning

confidence: 99%

Development of a Lateral Flow Kit for Detection of IgG and IgM Antibodies Against Rift Valley Fever Virus in Sheep

et al. 2019

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Rift Valley fever (RVF) is a viral zoonosis that primarily affects animals but also has the capacity to infect humans. The disease also results in significant economic losses due to death and abortion among RVF-infected livestock. A lateral flow device (LFD) was developed for the rapid detection of IgG and IgM anti-RVF antibodies in infected and vaccinated ovine sera. The conjugated RVF antigen with nano-gold was used as a detector antigen and was laid on a conjugated pad. The staphylococcal protein A was used as the capture complex (antibodies IgG-antigen conjugated with nanogold) at the first test line of device and anti-IgM antibodies were used as the capture complex (antibodies IgM-antigen conjugated with nanogold) at the second test line of device. When compared with RVF specific ELISA, The sensitivity, specificity, and accuracy of developed LFD for detection of IgG were found to be 88.2, 96.9 and 94.0%, respectively, while the same for detection of IgM was found to be 100, 96, and 96%, respectively.

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Development and evaluation of a rapid neutralizing antibody test for rabies

Cited by 32 publications

References 13 publications

A Comparative Study of the RAPINA and the Virus‐Neutralizing Test (RFFIT) for the Estimation of Antirabies‐Neutralizing Antibody Levels in Dog Samples

A Comparative Study of the RAPINA and the Virus‐Neutralizing Test (RFFIT) for the Estimation of Antirabies‐Neutralizing Antibody Levels in Dog Samples

Relationship between Virus-Neutralizing Antibody Levels and the Number of Rabies Vaccinations: a Prospective Study of Dogs in Japan

Development of a Lateral Flow Kit for Detection of IgG and IgM Antibodies Against Rift Valley Fever Virus in Sheep

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