Development and evaluation of a simple and easy high‐performance liquid chromatography–ultraviolet system simultaneously suitable for determination of 24 anti‐epileptic drugs in plasma

Abstract

We aim to establish a simple and easy high-performance liquid chromatography system coupled with an ultraviolet detector suitable for simultaneous determination of 24 antiepileptic drugs in human plasma. Optimized chromatographic separation was performed on a ZORBAX Eclipse Plus-C18 (4.6 × 150 mm 2 , 3.5 μm) column with acetonitrile and 5 mM potassium dihydrogen phosphate water solution as mobile phase. Note that, 24 antiepileptic drugs were divided into three groups and eluted with different gradient procedures, respectively. The column temperature was maintained at 35 • C and the detection wavelength was set at 210 nm. Plasma was processed with ethyl acetate or acetonitrile. The calibration curves of 24 antiepileptic drugs demonstrated good linearity within the test range (r > 0.996). The intra-and inter-batch precision and accuracy were all less than 15%, while extraction recoveries were in the range of 74.57-90.89% with the relative standard deviation values less than 15%. The validated methods have been successfully applied to determination of some antiepileptic drugs in rat or patient plasma. Those results indicated that the developed methods were simple and easy, and could be suitable for the determination of 24 antiepileptic drugs in plasma just by changing the gradient elution procedures of mobile phase.

Fabrication of a surface molecularly imprinted polymer membrane based on a single template and its application in the separation and extraction of phenytoin, phenobarbital and lamotrigine

“…High-performance liquid chromatography (HPLC) coupled with spectrometry has become an important technique for the monitoring of PHT, PHB or LTG in environmental water, urine, and plasma. 17,22,23 During this process, the extraction and separation of PHT, PHB or LTG from matrices is the rst and key step. Recently, solid-phase extraction (SPE) based on molecularly imprinted polymers has aroused comprehensive attention from scientists in the elds of environmental and biomedical analysis, [24][25][26] which has been successfully applied in the determination of PHT, PHB or LTG in environmental water, urine, serum, and plasma.…”

Antiepileptic drug concentration detection based on Raman spectroscopy and an improved snake optimization-convolutional neural network algorithm

“…14 Yanlin Zhao has established a simple and feasible high-performance liquid chromatography-ultraviolet detector system for the simultaneous determination of 24 antiepileptic drugs in human plasma. 15 Raman spectroscopy can be applied to drug concentration detection because of its advantages such as a convenient detection process, high accuracy of results, and short measurement time compared with other methods.…”

Ecofriendly appraisal of stability‐indicating high‐performance chromatographic assay of canagliflozin and metformin with their toxic impurities; in silico toxicity prediction

“…Liquid chromatography is still the most applicable technique of choice for the analysis of drugs, pharmaceuticals, amino acids, nucleic acids, carbohydrates, lipids, and polymers, [27][28][29][30][31]. Based on the aforementioned revealed date; the proposed HPTLC-densitometric method was a must as the first determination of CN, MT, their respective major degradation products, DG, CG, and MT toxic impurity, ML in laboratory prepared mixtures and pharmaceutically formulated combinations containing CN and MT after ICH validation and statistically comparing the proposed method's results with those of the reported method [9].…”