Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone

Jensen, Marie Aarrebo; Hansen, Åse Marie; Abrahamsson, Peter; Nørgaard, Asger W.

doi:10.1016/j.jchromb.2011.07.005

Cited by 84 publications

(46 citation statements)

References 54 publications

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“…Neither testosterone nor cortisol was correlated with the time interval between sampling and freezing (p's > .25). In line with this, previous studies have shown that saliva samples can be mailed and/or stored at room temperature for at least 7 days and sometimes up to 4 weeks without cortisol or testosterone levels being affected (Clements & Parker, 1998;Dabbs, 1991;Garde & Hansen, 2005;Gröschl, Wagner, Rauh, & Dörr, 2001;Jensen et al, 2011;Kirschbaum & Hellhammer, 1989.…”

Section: Methodssupporting

confidence: 55%

“…On two subsequent workdays, they collected one saliva sample at home after awakening. Self-sampling of saliva has been described as an adequate method for field studies (Garde & Hansen, 2005;Jensen, Hansen, Abrahamsson, & Nørgaard, 2011). It is widely used since it allows participants to collect samples non-invasively in their natural environment directly after awakening (see e.g., Chida & Steptoe, 2009;Gettler, McDade, & Kuzawa, 2011;Perini, Ditzen, Fischbacher, & Ehlert, 2012).…”

Section: Methodsmentioning

confidence: 99%

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Salivary testosterone and cortisol are jointly related to pro-environmental behavior in men

Sollberger

Bernauer

Ehlert

2015

Social Neuroscience

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Recently, cortisol has been suggested to moderate the positive relationship between testosterone and antisocial behavior. More precisely, high testosterone levels have been found to be related to aggressive or dominant behavior especially when cortisol levels were low. In the present study, we aimed to extend these findings to pro-environmental behavior as an indicator of prosocial behavior. In a first step, 147 male participants provided information on their everyday pro-environmental behavior by completing an online questionnaire on various energy-saving behaviors. In a second step, subjects provided two saliva samples for the assessment of testosterone and cortisol on two subsequent mornings after awakening. We found that testosterone was negatively related to pro-environmental behavior, but only in men with low cortisol. In conclusion, our findings provide first evidence for the joint association of testosterone and cortisol with everyday pro-environmental behavior. These results further reinforce the importance of considering interdependent hormone systems simultaneously rather than focusing on a single hormone.

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Section: Methodssupporting

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Salivary testosterone and cortisol are jointly related to pro-environmental behavior in men

Sollberger

Bernauer

Ehlert

2015

Social Neuroscience

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“…Cortisol excretion is coordinated via the hippocampus and the hypothalamicpituitary-adrenal axis [11]. There is a considerable affiliation between the plasma/serum and salivary levels of melatonin and cortisol [12].…”

Section: Introductionmentioning

confidence: 99%

Variance of melatonin and cortisol rhythm in patients with allergic rhinitis

Fidan

Hakan

Gözeler

et al. 2013

American Journal of Otolaryngology

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“…An LC/positive ESI-MS/ MS method published recently is able to quantify melatonin in saliva with the limit of detection of 4 pmol/L. 46) Using this method, salivary testosterone and cortisol, which also show significant diurnal rhythms, were determined simultaneously. The pilot study among four healthy volunteers proved that the method is useful for monitoring the circadian rhythms for the three hormones.…”

Section: Non-steroid Hormonesmentioning

confidence: 99%

Salivary Hormone Measurement Using LC/MS/MS: Specific and Patient-Friendly Tool for Assessment of Endocrine Function

Higashi

2012

Biological & Pharmaceutical Bulletin

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Saliva has recently been attracting attention as a patient-friendly available bio-fluid and an alternative to serum/plasma for hormone tests. LC coupled with atmospheric pressure ionization-MS/MS, especially electrospray ionization (ESI)-MS/MS, has been recently valued as a highly specific method in the analysis of salivary hormones. In this article, LC/ESI-MS/MS assays for salivary hormones are overviewed according to the papers that have been published during the last 5 years. Practical derivatization to enhance the detectabilities of hormones in ESI-MS/MS is also discussed, because a major disadvantage of using saliva is low hormone concentrations.

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Development and evaluation of a liquid chromatography tandem mass spectrometry method for simultaneous determination of salivary melatonin, cortisol and testosterone

Cited by 84 publications

References 54 publications

Salivary testosterone and cortisol are jointly related to pro-environmental behavior in men

Salivary testosterone and cortisol are jointly related to pro-environmental behavior in men

Variance of melatonin and cortisol rhythm in patients with allergic rhinitis

Salivary Hormone Measurement Using LC/MS/MS: Specific and Patient-Friendly Tool for Assessment of Endocrine Function

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