Development and evaluation of a selective medium for Brucella suis

Ferreira, Ana Cristina; Almendra, Cláudia; Cardoso, Regina; Pereira, Marta Silva; Beja‐Pereira, Albano; Luikart, Gordon; Sá, Maria Inácia Corrêa de

doi:10.1016/j.rvsc.2011.09.004

Cited by 11 publications

(9 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In OIE Cattle Brucellosis Chapter, too, using two media simultaneously to be able to augment the isolation sensitivity is recommended (17,11). Similarly, Ferreira et al (30) suggested using more than one selective medium to enhance the isolation sensitivity.…”

Section: Discussionmentioning

confidence: 99%

The Evaluation of Brucella Spp. Isolation Rates in Ruminant Abortion Cases by Using Different Selective Media

Karagül

İkiz

2018

Macedonian Veterinary Review

View full text Add to dashboard Cite

The aim of this study is to evaluate the success of Brucella spp. isolation in ruminant abortion cases by using different selective media. To this end, 58 samples from ruminant abortion cases were utilized. 4 selective media; namely, Farrell Medium (FM), CITA Medium (CM), Modified Thayer Martin (MTM) and Jones & Morgan (JM) were preferred for isolation. In addition to these, one medium with antibiotics was used to extend the range of the results. Suspensions prepared from organ and fetal stomach contents were inoculated to media plates and incubated at 37C° for 5-8 days in 5-10% CO2 condition. Conventional biotyping method was used to identify Brucella isolates within the level of species and biovar. MTM (67.2%) and Farrell (65.5%) outperformed the other media with regards to isolation rate. However, regarding the inhibition ability against contaminant microrganisms, Farrell (86.2%) and CITA (72%) have the highest and second highest percentages respectively. The media’s inhibition ability was examined in the samples in which Brucella spp. isolation occurred to be able to investigate the correlations between isolation and inhibition. Lower isolation percentage was observed in the samples in which the media displayed the lowest inhibition ability against contaminants. In this context, using two different selective media with high inhibition ability against contaminants may be recommended to enhance the isolation rate. Moreover, the components stimulating the growth of Brucella strains might be added to the media to obtain better results.

show abstract

Section: Discussionmentioning

confidence: 99%

The Evaluation of Brucella Spp. Isolation Rates in Ruminant Abortion Cases by Using Different Selective Media

Karagül

İkiz

2018

Macedonian Veterinary Review

View full text Add to dashboard Cite

show abstract

“…For this reason, negative culture result should not be considered as definitive evidence to rule out Brucella infection in an animal/herd [29,120]. In fact, the sensitivity of culture is highly variable, depending on the stage of infection, the specimens analyzed, and the number of samples cultured [100,102,118,[121][122][123]].…”

Section: Bacteriologymentioning

confidence: 99%

Control of Animal Brucellosis — The Most Effective Tool to Prevent Human Brucellosis

García‐Seco¹,

Domı́nguez²,

Álvarez³

2015

Updates on Brucellosis

View full text Add to dashboard Cite

The World Health Organization classifies brucellosis as one of the seven neglected endemic zoonosis which contribute to the perpetuation of poverty in developing countries. Although most of the developed countries are free from this important zoonosis, brucellosis has still a widespread distribution in the Mediterranean region, the Middle East, Central Asia, and parts of Latin America, making it a global problem. Nearly half a million of new cases of human brucellosis are reported each year around the world, in which animals (or products of animal origin) are the most likely source of infection. Brucella melitensis, the main etiologic agent of small ruminant brucellosis, is the most prevalent specie involved in cases of human disease in most parts of the world. Additionally, Brucella abortus (main responsible of bovine brucellosis) and Brucella suis (the most common etiological agent of porcine brucellosis) are often associated with human brucellosis. In animal production, brucellosis has a strong economic impact due not only to its direct consequences (e.g., reproductive failures) but also to indirect loses (e.g., trade restrictions). The problem of brucellosis could be considered a clear example of the need for a "One World, One Health" strategy, given that the only approach to achieve its control and subsequent eradication is the cooperation between public and animal health authorities. The prevention of human brucellosis cannot be achieved without the control of the disease in the animals, as exemplified by the impact that the early measures adopted in the beginning of the 20th century forbidding the consumption of goat milk had on the prevalence of the disease in the British soldiers in Malta. When the prevalence of the disease in the animal population is high or when eradication cannot be achieved due to other factors (e.g., lack of economic resources), its control in livestock must be the first objective. When deciding the optimal approach to tackle the disease, the prevalence of animal brucellosis is not the only parameter to consider by the decision makers since other epidemiological and economic aspects should be considered in order to implement the most adequate control strategy in each region. Cooperation between all stakeholders involved is a cornerstone in the success of any control strategy. Strict biosafety and management measures, vaccination, and test-and-slaughter strategy are recognized as the most ef

show abstract

“…B. suis is divided into five biovars, but only biovars 1, 2, and 3 are the etiological agents involved in swine brucellosis; B. suis biovar 2 is also found in wild boar ( Sus scrofa ), hares ( Lepus europaeus ) and biovar 3 in rodents (1). In Europe, biovar 2 is the most commonly isolated, and its prevalence in wild boars appears to be high throughout continental Europe, implicating them as a potential source of transmission to outdoor-reared pigs (1–3). B. suis biovar 2 strains PT09143 (haplotype 2e), PT09172 (haplotype 2d), and Bs143CITA (haplotype 2e) were isolated from wild boars, biotyped according to standard bacteriological procedures (4), and characterized through PCR-restriction fragment length polymorphism (RFLP) analysis for the omp2a , omp2b , and omp31 genes and a multilocus variable-number tandem-repeat (VNTR) assay (MLVA).…”

Section: Genome Announcementmentioning

confidence: 99%

Complete Genome Sequences of Three Iberian Brucella suis Biovar 2 Strains Isolated from Wild Boars

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

Development and evaluation of a selective medium for Brucella suis

Cited by 11 publications

References 10 publications

The Evaluation of Brucella Spp. Isolation Rates in Ruminant Abortion Cases by Using Different Selective Media

The Evaluation of Brucella Spp. Isolation Rates in Ruminant Abortion Cases by Using Different Selective Media

Control of Animal Brucellosis — The Most Effective Tool to Prevent Human Brucellosis

Complete Genome Sequences of Three Iberian Brucella suis Biovar 2 Strains Isolated from Wild Boars

Contact Info

Product

Resources

About