Development and Evaluation of an in vitro Detached Leaf Assay forc Pre-Screening Resistance to Fusarium Head Blight in Wheat

Browne, R. A.; Cooke, B. M.

doi:10.1023/b:ejpp.0000010143.20226.21

Cited by 72 publications

(116 citation statements)

References 17 publications

Supporting

Mentioning

104

Contrasting

Order By: Relevance

“…In the detached leaf assay, the partial disease resistance components such as incubation period (period from inoculation to the first appearance of symptoms), latent period (period from inoculation to sporulation) and lesion length are measured (Diamond & Cooke 1999;Browne & Cooke 2004;Browne et al 2006). Significant relationships were found between the FHB and seedling blight resistance in vitro, but such relationships were generally highly dependent on the cultivar, and therefore the in vitro test is likely the best means for measuring components of FHB resistance and/or genotype-specific resistance components (Brennan et al 2007).…”

Section: Methods For Evaluation Of Fhb Resistance In Small Grain Cerementioning

confidence: 99%

Cereal resistance to Fusarium head blight and possibilities of its improvement through breeding

Kosová¹,

Chrpová²,

Šíp³

2009

CZECH J GENET PLANT

View full text Add to dashboard Cite

The aim of this review is to summarize recent information on Fusarium head blight (FHB) in small grain cereals, especially in wheat and barley. Basic information on FHB epidemiology, types of resistance and plant resistance mechanisms is included. Standard methods for the evaluation of the individual types of FHB resistance and the extent of infection are briefly described. Special attention is paid to the sources of FHB resistance of different origin and possibility of their exploitation in cereal breeding. Unfortunately, a high level of FHB resistance was detected in non-adapted germplasm or distant relatives, which is a serious impediment to breeding progress in this field. The present state of breeding for FHB resistance in wheat, barley, rye, triticale and oats was analyzed. It was shown that large-scale QTL detections provide new opportunities for increasing the resistance; however, multi-step phenotypic selection still remains to be the most effective tool. Pedigree analyses indicated that the latest progress reached in this field was obtained through the cumulation of resistance genes coming from heterogeneous sources with different response to FHB.

show abstract

Section: Methods For Evaluation Of Fhb Resistance In Small Grain Cerementioning

confidence: 99%

Cereal resistance to Fusarium head blight and possibilities of its improvement through breeding

Kosová¹,

Chrpová²,

Šíp³

2009

CZECH J GENET PLANT

View full text Add to dashboard Cite

show abstract

“…Each leaf segment was placed adaxial surface uppermost on the surface of 0.5% water agar as described by Browne and Cooke (2004). Leaf segments were inoculated at the center of the adaxial surface with 5 μl inoculum suspension of 1 × 10 5 conidia • m -1 containing 0.05% (v/v) Tween 20.…”

Section: Plant Inoculation and Disease Evaluationmentioning

confidence: 99%

Evaluation of resistance and the role of some defense responses in wheat cultivars to Fusarium head blight

Khaledi¹,

Taheri²,

Falahati-Rastegar³

2018

Journal of Plant Protection Research

View full text Add to dashboard Cite

Fusarium graminearum and F. culmorum are the causal agents of Fusarium head blight (FHB) in cereal crops worldwide. Application of resistant cultivars is the most effective and economic method for management of FHB and reducing mycotoxin production in wheat. Understanding the physiological and biochemical mechanisms involved in basal resistance of wheat to FHB disease is limited. In this research, after screening resistance levels of eighteen wheat cultivars planted in Iran, Gaskozhen and Falat were identified as partially resistant and susceptible wheat cultivars against Fusarium spp., respectively. Also, we investigated the role of hydroxyl radical (OH − ), nitric oxide (NO), callose deposition, lipid peroxidation and protein content in basal resistance of wheat to the hemi-biotrophic and necrotrophic Fusarium species causing FHB. Nitric oxide as a signaling molecule may be involved in physiological and defensive processes in plants. Our results showed that NO generation increased in seedlings and spikes of wheat cultivars after inoculation with Fusarium species. We observed earlier and stronger callose deposition at early time points after infection by Fusarium spp. isolates than in non-infected plants, which was positively related to the resistance levels in wheat cultivars. Higher levels of OH − and malondialdehyde (MDA) accumulation (as a marker of lipid peroxidation) were observed in the Falat than in the Gaskozhen cultivar, under non-infected and infected conditions. So, estimation of lipid peroxidation could be useful to evaluate cultivars' susceptibility. These findings can provide novel insights for better recognition of physiological and biochemical markers of FHB resistance, which could be used for rapid screening of resistance levels in wheat cultivars against this destructive fungal disease.

show abstract

“…Detached leaf disease experiments were performed as described previously (Browne and Cooke, 2004), with some modifications. An 8-cm section was cut from the second leaf of three-leaf-stage plants (growth stage 13; Zadoks et al, 1974).…”

Section: Detached Leaf Disease Assessment Studymentioning

confidence: 99%

TaFROG encodes a Pooideae orphan protein that interacts with SnRK1 and enhances resistance to the mycotoxigenic fungus Fusarium graminearum

et al. 2015

View full text Add to dashboard Cite

Development and Evaluation of an in vitro Detached Leaf Assay forc Pre-Screening Resistance to Fusarium Head Blight in Wheat

Cited by 72 publications

References 17 publications

Cereal resistance to Fusarium head blight and possibilities of its improvement through breeding

Cereal resistance to Fusarium head blight and possibilities of its improvement through breeding

Evaluation of resistance and the role of some defense responses in wheat cultivars to Fusarium head blight

TaFROG encodes a Pooideae orphan protein that interacts with SnRK1 and enhances resistance to the mycotoxigenic fungus Fusarium graminearum

Contact Info

Product

Resources

About