Development and evaluation of an indirect enzyme-linked immunosorbent assay based on a recombinant SifA protein to detect Salmonella infection in poultry

Gao, D.; Yu, Jiangxu; Dai, Xinyan; Tian, Yanhong; Sun, Jian; Xu, Xiaojuan; Cai, Xuwang

doi:10.1016/j.psj.2023.102513

Cited by 9 publications

(3 citation statements)

References 34 publications

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“…Immunoglobulin isotypes IgM, IgG, and complement 3 were assayed using enzyme-linked immunosorbent assay ( ELISA ) technique ( Gao et al, 2023 ).…”

Section: Methodsmentioning

confidence: 99%

The potential of Spirulina platensis to substitute antibiotics in Japanese quail diets: impacts on growth, carcass traits, antioxidant status, blood biochemical parameters, and cecal microorganisms

Alghamdi,

Reda,

Mahmoud

et al. 2024

Poultry Science

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“…Immunoglobulin isotypes IgM, IgG, and complement 3 were assayed using enzyme-linked immunosorbent assay ( ELISA ) technique ( Gao et al, 2023 ).…”

Section: Methodsmentioning

confidence: 99%

The potential of Spirulina platensis to substitute antibiotics in Japanese quail diets: impacts on growth, carcass traits, antioxidant status, blood biochemical parameters, and cecal microorganisms

Alghamdi,

Reda,

Mahmoud

et al. 2024

Poultry Science

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“…Immunoglobulin isotypes IgM, IgG, were assayed using enzyme-linked immunosorbent assay ( ELISA ) technique ( Gao et al, 2023 ). Lysozyme activity, an enzyme that contributes in the fight against infection, was assessed by taking blood samples at the end of the trial.…”

Section: Methodsmentioning

confidence: 99%

The incorporation of sumac seed powder (Rhus coriaria L.) into the diet of quail breeders as a novel feed additive

Reda,

Alagawany,

Alsolami

et al. 2024

Poultry Science

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“…Alternative analytical methods are those based on the immunoanalytical detection of foodborne pathogens using specific antibodies, first of all, enzyme-linked immunosorbent assay (ELISA), which provides sensitive and selective detection of bacteria, including Salmonella [11][12][13][14]. However, a typical ELISA contains several steps and requires some laboratory equipment; therefore, it cannot be classified as a rapid on-site method.…”

Section: Introductionmentioning

confidence: 99%

Sensitive Immunochromatographic Determination of Salmonella typhimurium in Food Products Using Au@Pt Nanozyme

Hendrickson,

Byzova,

Safenkova

et al. 2023

Nanomaterials

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In this study, we developed a sensitive immunochromatographic analysis (ICA) of the Salmonella typhimurium bacterial pathogen contaminating food products and causing foodborne illness. The ICA of S. typhimurium was performed using Au@Pt nanozyme as a label ensuring both colorimetric detection and catalytic amplification of the analytical signal due to nanozyme peroxidase-mimic properties. The enhanced ICA enabled the detection of S. typhimurium cells with the visual limit of detection (LOD) of 2 × 102 CFU/mL, which outperformed the LOD in the ICA with traditional gold nanoparticles by two orders of magnitude. The assay duration was 15 min. The specificity of the developed assay was tested using cells from various Salmonella species as well as other foodborne pathogens; it was shown that the test system detected only S. typhimurium. The applicability of ICA for the determination of Salmonella in food was confirmed in several samples of milk with different fat content, as well as chicken meat. For these real samples, simple pretreatment procedures were proposed. Recoveries of Salmonella in foodstuffs were from 74.8 to 94.5%. Due to rapidity and sensitivity, the proposed test system is a promising tool for the point-of-care control of the Salmonella contamination of different food products on the whole farm-to-table chain.

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Development and evaluation of an indirect enzyme-linked immunosorbent assay based on a recombinant SifA protein to detect Salmonella infection in poultry

Cited by 9 publications

References 34 publications

The potential of Spirulina platensis to substitute antibiotics in Japanese quail diets: impacts on growth, carcass traits, antioxidant status, blood biochemical parameters, and cecal microorganisms

The potential of Spirulina platensis to substitute antibiotics in Japanese quail diets: impacts on growth, carcass traits, antioxidant status, blood biochemical parameters, and cecal microorganisms

The incorporation of sumac seed powder (Rhus coriaria L.) into the diet of quail breeders as a novel feed additive

Sensitive Immunochromatographic Determination of Salmonella typhimurium in Food Products Using Au@Pt Nanozyme

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