Development and Evaluation of Cell Culture Devices with the Gas-permeable Membrane

Design considerations of benchtop fluid flow bioreactors for bio-engineered tissue equivalents in vitro

“…The vessel used to hold cultures can lead to variability in oxygen levels due to factors such as the diffusion of oxygen through the vessel walls. This effect has been investigated for increasing the oxygen levels using highly gas-permeable materials in both static and dynamic culture systems [135] , [136] , [137] , [138] . The depth of the culture within the medium also leads to changes in oxygen concentration, with the major limiting factor being the diffusion rate through the media, the further a culture is from the air the lower the dissolved oxygen levels will be.…”