Development and evaluation of serological assays for detection of Hantaan virus-specific antibodies in human sera using yeast-expressed nucleocapsid protein

Petraityte, Rasa; Yang, Hua; Hunjan, Rashpal; Ražanskienė, Aušra; Dhanilall, Pravesh; Ulrich, Rainer G.; Sasnauskas, Kęstutis; Li, Jin

doi:10.1016/j.jviromet.2007.10.019

Cited by 7 publications

(6 citation statements)

References 35 publications

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“…Generally, an IgM capture ELISA is widely used for serodiagnosis of disease because low levels of specific IgM antibodies can be detected without interference of IgG antibodies (Porter et al, 1999). In our study, indirect IgM ELISA was used to evaluate the seroreactivity of CHIKV capsid protein and we observed that the sensitivity and specificity of indirect ELISA were high, consistent with the findings of a previous report (Petraityte et al, 2008). However, indirect IgM ELISA could not detect 6 of 40 anti-CHIKV-positive sera as positive even though those sera samples did show very low seroreactivity (data from Laborotoire Marcel Merieux (Lyon, France).…”

Section: Evaluation Of the Recombinant Chikv Capsid Protein Using Icasupporting

confidence: 90%

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

Cho¹,

Kim²,

Cho

et al. 2008

Journal of Virological Methods

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Section: Evaluation Of the Recombinant Chikv Capsid Protein Using Icasupporting

confidence: 90%

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

Cho¹,

Kim²,

Cho

et al. 2008

Journal of Virological Methods

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“…In line with data obtained for yeast-expressed HTNV antigen [83,84], we found a very similar sensitivity and specificity of the PUUV-Bawa antigen-based indirect ELISA (99 and 100%) and mAb-capture ELISA (95 and 100%) for the serum panel from Germany. A comparison of the results of the validation of the indirect PUUV-Vra ELISA and the PUUV-Bawa mAb-capture ELISA for all investigated sera from Finland showed a lower sensitivity for the capture ELISA (89%) than for the indirect ELISA (100%), but a higher specificity (100 and 95%, respectively).…”

Section: Reactivity Of Human Sera and Mabs With Different Puuv N Antisupporting

confidence: 89%

Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein

et al. 2011

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Puumala virus (PUUV) is the predominant hantavirus species in Germany causing large numbers of mild to moderate cases of haemorrhagic fever with renal syndrome (HFRS). During an outbreak in South-East Germany in 2004 a novel PUUV subtype designated Bavaria was identified as the causative agent of HFRS in humans [1]. Here we present a molecular characterization of this PUUV strain by investigating novel partial and almost entire nucleocapsid (N) protein-encoding small (S-) segment sequences and partial medium (M-) segment sequences from bank voles (Myodes glareolus) trapped in Lower Bavaria during 2004 and 2005. Phylogenetic analyses confirmed their classification as subtype Bavaria, which is further subdivided into four geographical clusters. The entire N protein, harbouring an amino-terminal hexahistidine tag, of the Bavarian strain was produced in yeast Saccharomyces cerevisiae and showed a slightly different reactivity with N-specific monoclonal antibodies, compared to the yeast-expressed N protein of the PUUV strain Vranica/Hällnäs. Endpoint titration of human sera from different parts of Germany and from Finland revealed only very slight differences in the diagnostic value of the different recombinant proteins. Based on the novel N antigen indirect and monoclonal antibody capture IgG-ELISAs were established. By using serum panels from Germany and Finland their validation demonstrated a high sensitivity and specificity. In summary, our investigations demonstrated the Bavarian PUUV strain to be genetically divergent from other PUUV strains and the potential of its N protein for diagnostic applications.

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“…[23,24,29,30] and indirect ELISAs for SNV, PUUV, HTNV and ANDV using E. coliexpressed N antigens [8,21,34]. The seroepidemiological study in four counties of two administrative districts of Lower Bavaria revealed an overall prevalence of 6.7% with a range of 0% in Schöfweg (district Freyung-Grafenau), 4.1% in Geiersthal (district Regen), 5.9% in Kirchberg (district Regen) and 11.9% in Spiegelau (district Freyung-Grafenau).…”

Section: Discussionmentioning

confidence: 99%

Seroepidemiological study in a Puumala virus outbreak area in South-East Germany

Mertens

Wölfel

Ullrich³

et al. 2009

Med Microbiol Immunol

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Puumala virus (PUUV) is the cause of the majority of haemorrhagic fever with renal syndrome cases in Germany. In 2004, a nephropathia epidemica outbreak was recorded in Lower Bavaria, South-East Germany. For a seroepidemiological study in this region including the resident population at four locations (n = 178) and soldiers from one location (n = 208) indirect immunoglobulin M (IgM) and immunoglobulin G (IgG) enzyme-linked immunosorbent assays (ELISAs) and immunoblot tests based on a yeast-expressed PUUV nucleocapsid protein were established. The validation using human serum panels originating from Germany revealed a diagnostic sensitivity and specificity of 98/100% for the IgM ELISA, 99/99% for the IgG ELISA, 99/100% for the IgM immunoblot test and 100/96% for the IgG immunoblot test. Using the novel IgG assays as well as a commercial IgG ELISA and an immunofluorescence assay for the resident population an average prevalence of 6.7% (12 of 178) with a range of 0% (0 of 21) to 11.9% (7 of 59) was observed. Positive serological results were equally distributed between males and females with an average age of 63 for males and 52 for females. The seroprevalence in the soldier group was found to be about 1% with one positive male of 203 (age 46 years) and one positive female of five (age 47 years). In conclusion, the PUUV seroprevalence in the residents of the outbreak region in Lower Bavaria was found to be up to fivefold higher than the average hantavirus seroprevalence of the German population.

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Development and evaluation of serological assays for detection of Hantaan virus-specific antibodies in human sera using yeast-expressed nucleocapsid protein

Cited by 7 publications

References 35 publications

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease

Phylogenetic analysis of Puumala virus subtype Bavaria, characterization and diagnostic use of its recombinant nucleocapsid protein

Seroepidemiological study in a Puumala virus outbreak area in South-East Germany

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