Development and in vitro characterisation of novel bioresorbable and bioactive composite materials based on polylactide foams and Bioglass® for tissue engineering applications

Roether, Judith A.; Boccaccını, Aldo R.; Hench, Larry L.; Maquet, Véronique; Gautier, Sandrine; Jérôme, Robert

doi:10.1016/s0142-9612(02)00131-x

Cited by 340 publications

(249 citation statements)

References 35 publications

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“…Composite scaffolds comprising of PDLLA with Bioglass ® particle additions are of current interest for bone tissue engineering (Blaker et al 2003;Blaker et al 2005;Maquet et al 2003;Maquet et al 2004;Roether et al 2002;Yang et al 2006). Adipose tissue is an abundant source of mesenchymal stem cells, which have shown promise in the field of regenerative medicine (Hicok et al 2004;Lalande et al 2011;Parker and Katz 2006).…”

Section: Discussionmentioning

confidence: 99%

Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Kirkham

et al. 2014

Cell Tissue Res

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Translational research in bone tissue engineering is essential for 'bench to bedside' patient benefit. However, the idea combination of stem cells and biomaterial scaffolds for bone repair/regeneration is still unclear. The aim of this study was to investigate the osteogenic capacity of a combination of poly(DL-lactic acid) (PDLLA) porous foams containing 5 and 40 wt% of Bioglass ® particles with human adipose-derived stem cells (ADSCs) in vitro and in vivo. Live/dead fluorescent markers, confocal microscopy and scanning electron microscopy (SEM) showed that PDLLA/Bioglass ® porous scaffolds supported ADSCs attachment, growth and osteogenic differentiation, which were confirmed by enhanced alkaline phosphatase activity (ALP). Higher Bioglass ® content of the PDLLA foams increased more ALP activity compared to PDLLA only group. Extracellular matrix deposition after 8 weeks in in vitro culture was evident by Alcian blue/Sirius red staining.In vivo bone formation was assessed using scaffold/ADSCs constructs in diffusion chambers transplanted intraperitoneally into nude mice and recovered after 8 weeks.Histological and immunohistochemical assays indicated significant new bone formation in the 40 wt% and 5 wt% Bioglass ® constructs compared to the PDLLA only group. This study indicated the combination of a well-developed biodegradable bioactive porous PDLLA/Bioglass ® composite scaffold with a high potential stem cells source -human ADSCs could be a promising approach for bone regeneration in clinical setting.

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Section: Discussionmentioning

confidence: 99%

Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Kirkham

et al. 2014

Cell Tissue Res

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“…In situ apatite formation can also be induced by a biomimetic process in which polymer foams were incubated into a simulated body fluid [21]. In recent studies [22,23], particles of 45S5 bioglass ® , a commercially available bioactive glass powder (US Biomaterials), have been used to produce bioactive coatings on commercially available sutures (Vicryls) and on PDLLA foams. More recently, we have described the preparation, characterisation and in vitro degradation of porous composites made of high molecular weight PDLLA and bioglass ® by phase separation and freeze-drying [24].…”

Section: Introductionmentioning

confidence: 99%

Porous poly(α-hydroxyacid)/Bioglass® composite scaffolds for bone tissue engineering. I: preparation and in vitro characterisation

et al. 2004

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Highly porous composites scaffolds of poly-D,L-lactide (PDLLA) and poly(lactide-co-glycolide) (PLGA) containing different amounts (10, 25 and 50wt%) of bioactive glass (45S5 bioglass ® ) were prepared by thermally induced solid-liquid phase separation (TIPS) and subsequent solvent sublimation. The addition of increasing amounts of bioglass ® into the polymer foams decreased the pore volume. Conversely, the mechanical properties of the polymer materials were improved. The composites were incubated in phosphate buffer saline at 37°C to study the in vitro degradation of the polymer by measurement of water absorption, weight loss as well as changes in the average molecular weight of the polymer and in the pH of the incubation medium as a function of the incubation time. The addition of bioglass ® to polymer foams increased the water absorption and weight loss compared to neat polymer foams. However, the polymer molecular weight, determined by size exclusion chromatography, was found to decrease more rapidly and to a larger extent in absence of bioglass ® . The presence of the bioactive filler was therefore found to delay the degradation rate of the polymer as compared to the neat polymer foams. Formation of hydroxyapatite on the surface of composites, as an indication of their bioactivity, was recorded by EDXA, X-ray diffractometry and confirmed by Raman spectroscopy.

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“…One group of materials being considered as scaffolds for bone tissue engineering are porous degradable polylactide, polyglycolide or their co-polymers [6,7] with additions of inorganic particles or fibres, such as bioactive glass [8][9][10] and hydroxy apatite (HA) [11], to impart bioactivity, control degradation kinetics and enhance the mechanical properties. A system currently being developed is based on poly(D,L-lactide) (PDLLA)/Bio-glass®-filled composite foams produced by thermally induced phase separation (TIPS) [12].…”

Section: Introductionmentioning

confidence: 99%

Mechanical properties of highly porous PDLLA/Bioglass® composite foams as scaffolds for bone tissue engineering

et al. 2005

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This study developed highly porous degradable composites as potential scaffolds for bone tissue engineering. These scaffolds consisted of poly-D,L-lactic acid filled with 2 and 15 vol.% of 45S5 Bioglass® particles and were produced via thermally induced solid-liquid phase separation and subsequent solvent sublimation. The scaffolds had a bimodal and anisotropic pore structure, with tubular macro-pores of ~100 µm in diameter, and with interconnected micro-pores of ~10-50 µm in diameter. Quasi-static and thermal dynamic mechanical analysis carried out in compression along with thermogravimetric analysis was used to investigate the effect of Bioglass® on the properties of the foams. Quasi-static compression testing demonstrated mechanical anisotropy concomitant with the direction of the macro-pores. An analytical modelling approach was applied, which demonstrated that the presence of Bioglass® did not significantly alter the porous architecture of these foams and reflected the mechanical anisotropy which was congruent with the scanning electron microscopy investigation. This study found that the Ishai-Cohen and Gibson-Ashby models can be combined to predict the compressive modulus of the composite foams. The modulus and density of these complex foams are related by a power-law function with an exponent between 2 and 3.

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Development and in vitro characterisation of novel bioresorbable and bioactive composite materials based on polylactide foams and Bioglass® for tissue engineering applications

Cited by 340 publications

References 35 publications

Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells

Porous poly(α-hydroxyacid)/Bioglass® composite scaffolds for bone tissue engineering. I: preparation and in vitro characterisation

Mechanical properties of highly porous PDLLA/Bioglass® composite foams as scaffolds for bone tissue engineering

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