Development and Interlaboratories Validation of Event-Specific Quantitative Real-Time PCR Method for Genetically Modified Rice G6H1 Event

Abstract

The transgenic rice G6H1 was a new event with the traits of herbicide-tolerance and insect-resistant. Herein, we developed one event-specific real-time PCR method with high specificity and sensitivity for G6H1 event quantitative analysis, and validated its performance on practical samples quantification through a collaborative ring trial. A total of eight laboratories participated in this validation and quantified three blind G6H1 powder samples including DNA extraction and real-time PCR analysis. The statistically analyzed results from returned data confirmed its high PCR efficiency and good linearity, trueness, and precision, indicating that the developed G6H1 real-time PCR assay was accurate, reliable, and comparable for G6H1 identification and quantification.

Identification of the Pol Gene as a Species-Specific Diagnostic Marker for Qualitative and Quantitative PCR Detection of Tricholoma matsutake

“…Rapid developments in modern biotechnology have enabled the wide use of molecular biological methods for authentication due to their accuracy, convenience, and speed. PCR-based methods, in particular, have been broadly adopted by many laboratories and fields [11,12]. Compared with conventional PCR, quantitative PCR technology has realized the leap of PCR from qualitative to quantitative, and it has higher specificity, effective resolution and higher degree of automation, and widely used in many fields such as gene expression research, transgenic research, drug efficacy assessment, pathogen detection and food composition analysis, especially.…”