Development and linkage mapping of E-STS and RGA markers for functional gene homologues in apple

Naik, S.; Hampson, C.; Gašić, Ksenija; Bakkeren, Guus; Korban, Schuyler S.

doi:10.1139/g06-085

Cited by 35 publications

(33 citation statements)

References 53 publications

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“…The apple molecular map currently has more than 900 markers on 17 linkage groups (Maliepaard et al, 1998;Liebhard et al, 2002Liebhard et al, , 2003aLiebhard et al, , 2003bXu and Korban, 2002b;Naik et al, 2006). Several efforts are under way to develop a substantial resource of molecular markers for genotyping and marker-assisted selection (MAS; Tartarini et al, 2000;Liebhard et al, 2002Liebhard et al, , 2003aLiebhard et al, , 2003bSilfverberg-Dilworth et al, 2006).…”

Section: Applementioning

confidence: 99%

Multiple Models for Rosaceae Genomics

et al. 2008

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Section: Applementioning

confidence: 99%

Multiple Models for Rosaceae Genomics

et al. 2008

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“…Several sequencing projects have generated more than 250,000 expressed sequence tags (ESTs) in apple over the past few years (Newcomb et al 2006, Naik et al 2006. SSRs associated with these sequences constitute a large resource of markers which have remained largely unexploited.…”

Section: Introductionmentioning

confidence: 99%

Development of novel EST-SSR markers derived from Japanese pear (Pyrus pyrifolia)

et al. 2009

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We developed 73 novel expressed sequence tag (EST)-simple sequence repeat (SSR) markers based on ESTs derived from 11 different cDNA libraries of the Japanese pear (Pyrus pyrifolia Nakai) cultivar 'Housui', comprising 30, 36, two, one, and four SSRs with repeat motifs of di-, tri-, tetra-, penta-, and hexanucleotides, respectively. Forty-eight EST sequences showed significant homology to Arabidopsis thaliana genes. The linkage groups and positions of 57 loci generated from 53 SSR markers covering all 17 linkage groups, were identified. These EST-SSRs will be useful for high-throughput genotyping of marker-assisted selection. The numbers of alleles and the observed and expected heterozygosities at single-locus EST-SSR markers in ten Japanese and ten European pears suggest high polymorphism and heterozygosity of the ESTSSRs. Di-nucleotide EST-SSRs showed two to three times the polymorphism and heterozygosity of trinucleotide EST-SSRs. Some of the EST-SSRs proved transferable to other species in the Rosaceae, paving the way for the development of markers for the same phenotypic traits among species of the Rosaceae.

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“…The percentage resistant seedlings in these two families changed to 55 and 88%, respectively, in 2004, and 32 and 37% in 2005, rather than the 50% one would expect for a dominant gene in heterozygous form in Antonovka. The major-gene hypothesis was also not supported by mapping studies of the Antonovka debnicka )Summerred population generated earlier (Quamme et al 2003;Naik et al 2006). Although the nature of resistance in these two Antonovka variants remains unclear, it was consistently transmitted to a high proportion of their progeny ( Fig.…”

Section: Resultsmentioning

confidence: 97%

“…1999; Gardiner et al 2003;Quamme et al 2003;Tartarini et al 2004;Calenge et al 2005;Naik et al 2006). Molecular markers are now available for pyramiding several major resistance genes (Bus et al 2005a, b;Boudichevskaia et al 2006;Gessler et al 2006).…”

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A study of scab resistance in 16 apple progenies using parents with partial scab resistance

Hampson¹,

Quamme²,

Sholberg³

2009

Can. J. Plant Sci.

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. 2009. A study of scab resistance in 16 apple progenies using parents with partial scab resistance. Can. J. Plant Sci. 89: 693Á699. A study was conducted to examine the inheritance of partial resistance to apple scab [Venturia inaequalis (Cke.) Wint.] using as parents a set of apple (Malus )domestica Borkh.) cultivars that show a range of field resistance to the disease, but are not known to carry any major-gene resistance. Akane, Alkmene, Antonovka, Carola, Chehalis, Goldgelbe, Katja and Summerred were used as pollen parents, and Spartan and an unnamed selection of Splendour )Gala parentage were used as seed parents in a factorial 2 )8 mating design. One hundred seedlings of each family were planted in a randomized complete block design at a site with heavy annual natural scab infections. Each seedling was rated for infection severity for 3 consecutive years using a six-point categorical scale. A x 2 procedure was used to estimate the contribution of each parent to field resistance (analogous to general combining ability, GCA) and the contribution of the particular male-female combination (analogous to specific combining ability, SCA). Infection severity tended to increase over the 3 yr of the study. Antonovka and Chehalis had the highest analogous GCA for resistance (defined here as absence of sporulating lesions). Specific combining ability was present in 2 of 3 yr, but was small in magnitude relative to GCA. The field resistance of a given parent tended to be a poor predictor of its breeding value. However, 13 of the 16 families had at least 30% of seedlings with a potentially useful degree of partial resistance (low severity of infection). The results are discussed in the context of breeding apples with durable scab resistance. . Ils ont utilise´pour cela les parents d'un groupe de cultivars de pommier (Malus )domestica Borkh.) affichant une re´sistance variable a`la maladie sur le terrain, mais qu'on ignorait porter ou pas d'importants ge`nes de re´sistance. Les cultivars Akane, Alkmene, Antonovka, Carola, Chehalis, Goldgelbe, Katja et Summerred ont servi a`produire le pollen qui a fertilise´la varie´teŚ partan et une se´lection sans nom d'ascendance Splendour )Gala pour la production de graines, dans le cadre d'un essai de reproduction factoriel 2 )8. Cent plantules de chaque famille ont ensuite e´te´repique´s au hasard en blocs ale´atoires complets, a`un endroit marque´par d'importantes infections naturelles de tavelure chaque anne´e. Chaque plantule a e´teń ote´e selon la gravite´de l'infection pendant trois anne´es conse´cutives, au moyen d'une e´chelle a`6 points. Une technique x 2 a permis d'estimer l'apport de chaque parent a`la re´sistance sur le terrain (valeur analogue a`l'aptitude ge´ne´rale a`la combinaison, GCA) et la contribution du couple maˆle-femelle particulier (valeur analogue a`l'aptitude spe´cifique a`la combinaison, SCA). Le degre´d'infection a eu tendance a`augmenter au cours des trois anne´es qu'a dure´es l'e´tude. Les cultivars Antonovka et Chehalis pre´sentaient la plus forte val...

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Development and linkage mapping of E-STS and RGA markers for functional gene homologues in apple

Cited by 35 publications

References 53 publications

Multiple Models for Rosaceae Genomics

Multiple Models for Rosaceae Genomics

Development of novel EST-SSR markers derived from Japanese pear (Pyrus pyrifolia)

A study of scab resistance in 16 apple progenies using parents with partial scab resistance

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