Development and molecular characterization of wheat-<i>Aegilops peregrina</i>Introgression Lines with resistance to leaf rust and stripe rust

Narang, Deepika; Kaur, Satinder; Saini, J.S.; Chhuneja, Parveen

doi:10.1101/160648

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“…All the seedlings in 69 families showed resistant infection type (;) and these RILs were classified as homozygous resistant, while all the seedlings in 81 families showed infection type 33+ and these RILs were classified as homozygous susceptible (Fig. 1) 22 . Ten families were found to to be segregating for the leaf rust resistance gene.…”

Section: Resultsmentioning

confidence: 99%

Discovery and characterisation of a new leaf rust resistance gene introgressed in wheat from wild wheat Aegilops peregrina

Narang

Kaur

Steuernagel

et al. 2020

Sci Rep

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Wild wheat species Aegilops peregrina (UpUpSpSp), harbours resistance to various diseases including leaf rust and stripe rust. Inheritance studies in a recombinant inbred line population of wheat-Ae. peregrina introgression line IL pau16061 revealed the transfer of a single major dominant gene conditioning all stage resistance, herein temporarily designated as LrAp. Genomic in situ hybridisation of IL pau16061, resistant and susceptible RILs with U- and S-genome DNA probes confirmed that the introgression with leaf rust resistance is from the Up genome of Ae. peregrina. Fluorescence in situ hybridisation using chromosome specific probes identified Up genome introgression to be on the long arm of wheat chromosome 6B. To genetically map LrAp, bulked segregant analysis was combined with resistance gene enrichment sequencing (MapRenSeq). Five nucleotide binding leucine-rich repeat contigs distinguished resistant and susceptible bulks and single nucleotide polymorphism (SNP) markers from these contigs co-segregated with LrAp. All five RenSeq NB_ARC contigs showed identity with the long arm of wheat chromosome 6B confirming the introgression on 6BL which we propose is a compensating translocation from Ae. peregrina chromosome 6UpL due to homoeology between the alien and wheat chromosomes. The SNP markers developed in this study will aid in cloning and marker assisted gene pyramiding of LrAp.

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Section: Resultsmentioning

confidence: 99%

Discovery and characterisation of a new leaf rust resistance gene introgressed in wheat from wild wheat Aegilops peregrina

Narang

Kaur

Steuernagel

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

Development and molecular characterization of wheat-Aegilops peregrinaIntrogression Lines with resistance to leaf rust and stripe rust

Abstract: A wild non-progenitor species from wheat tertiary gene pool Aegilops peregrina accession pau3519 (UUSS) was used for introgression of leaf rust and stripe rust resistance in bread

Cited by 1 publication

References 25 publications

Discovery and characterisation of a new leaf rust resistance gene introgressed in wheat from wild wheat Aegilops peregrina

Discovery and characterisation of a new leaf rust resistance gene introgressed in wheat from wild wheat Aegilops peregrina

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