Development and molecular cytogenetic characterization of Thinopyrum bessarabicum introgression lines in hexaploid and tetraploid wheats

Singh, Amit Kumar; Zhang, Peng; Dong, Chongmei; Li, Jianbo; Singh, Smriti; Trethowan, Richard; Sharp, P. J.

doi:10.1007/s00122-020-03581-5

Cited by 6 publications

(3 citation statements)

References 45 publications

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“…Unlike diploid plants, F 1 population of a cross between two sugarcane parents segregates in both phenotype and genotype with varied numbers of chromosomes in the individuals. To corelate the phenotype, karyotype analysis is often used to characterize the chromosomes in the F 1 progenies [ 7 , 13 ].Karyotype analysis can not only be used to study the genetic variation, systematic evolution and genetic relationship between species but can also be used to inform cross-breeding and breeding [ 14 ] and provide a theoretical basis for the identification of hybrid offspring. Understanding chromosome behavior in intergeneric hybrids, such as chromosome elimination and unreduced gametes resulting from different coexisting chromosomes, is important for practical application in introgression programs [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

“…Understanding chromosome behavior in intergeneric hybrids, such as chromosome elimination and unreduced gametes resulting from different coexisting chromosomes, is important for practical application in introgression programs [ 15 ]. Sequential fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH) are techniques that can be used as part of a robust approach for the precise identification of alien chromatin within sugarcane [ 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of Saccharum spp.

Wang

Chen

2023

Plants

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Sugarcane smut is a worldwide fungal disease. Disease resistance breeding is the most economical and effective measure to prevent and control sugarcane smut. The cytogenetic characteristics and metabolomic differences of sugarcane F1s are closely related to disease resistance. Zhongzhe 1 and G160 sugarcane from the same parents (ROC25 and Yunzhe89-7) were used; the plants were grown in accordance with the barrel method. When the seedlings had 4–5 leaves, genomic in situ hybridization (GISH) was performed; digoxigenin (DIG)-labeled female parental (ROC25)DNA and biotin-labeled male parental (Yunzhe89-7) DNA were used as probes, and the karyotypes of two hybrids were analyzed. The new sugarcane smut-resistant variety (Zhongzhe 1) and the susceptible variety (G160) derived from the same parent were analyzed via gas chromatography‒mass spectrometry technology (GC‒MS) to compare the metabolomic differences between them. GISH analysis revealed that the chromosome ploidy number of Zhongzhe 1 sugarcane and G160 sugarcane were 114 and 110, respectively. However, the two contain different numbers of chromosomes from the female (ROC25) and male (Yunzhe89-7) parents. Moreover, 258 significantly changed metabolites were identified in smut-resistant Zhongzhe 1, as compared with the smut-susceptible G160 sugarcane: 56 flavonoids, 52 phenolic acids, 30 lipids, 26 organic acids, 26 amino acids and derivatives, 19 nucleotides and derivatives, 5 alkaloids, 9 terpenoids, and 35 others. Multivariate statistical analysis revealed a distinct difference in metabolic pathways between Zhongzhe 1 sugarcane and G160, and both of these varieties had unique functional metabolites. Differences in chromosome composition may constitute the genetic basis for the difference in resistance to smut disease between Zhongzhe 1 sugarcane and G160 sugarcane, and a high accumulation of flavonoids, lipids, terpenoids and tannins may constitute the basis of resistance to smut disease for the Zhongzhe 1 variety.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of Saccharum spp.

Wang

Chen

2023

Plants

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“…For introgression of the genes from wheatgrass, the partial wheat–wheatgrass amphidiploids, or hybrids (WWGH) are used. WWGHs carry a combination of wheat and wheatgrass genomes, which makes it possible to use them as breeding bridges to facilitate the transfer of genes of interest from wheatgrass to wheat [ 18 , 19 , 20 , 21 , 22 , 23 ]. Many partial WWGHs possess unique composition of wheatgrass chromosomes associated with agronomically valuable traits [ 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Development of Specific Thinopyrum Cytogenetic Markers for Wheat-Wheatgrass Hybrids Using Sequencing and qPCR Data

Nikitina

Kuznetsova

Kroupin

et al. 2020

IJMS

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The cytogenetic study of wide hybrids of wheat has both practical and fundamental values. Partial wheat-wheatgrass hybrids (WWGHs) are interesting as a breeding bridge to confer valuable genes to wheat genome, as well as a model object that contains related genomes of Triticeae. The development of cytogenetic markers is a process that requires long and laborious fluorescence in situ hybridization (FISH) testing of various probes before a suitable probe is found. In this study, we aimed to find an approach that allows to facilitate this process. Based on the data sequencing of Thinopyrum ponticum, we selected six tandem repeat (TR) clusters using RepeatExplorer2 pipeline and designed primers for each of them. We estimated the found TRs’ abundance in the genomes of Triticum aestivum, Thinopyrum ponticum, Thinopyrum intermedium and four different WWGH accessions using real-time qPCR, and localized them on the chromosomes of the studied WWGHs using fluorescence in situ hybridization. As a result, we obtained three tandem repeat cytogenetic markers that specifically labeled wheatgrass chromosomes in the presence of bread wheat chromosomes. Moreover, we designed and tested primers for these repeats, and demonstrated that they can be used as qPCR markers for quick and cheap monitoring of the presence of certain chromosomes of wheatgrass in breeding programs.

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The launch of satellite: DNA repeats as a cytogenetic tool in discovering the chromosomal universe of wild Triticeae

et al. 2023

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Development and molecular cytogenetic characterization of Thinopyrum bessarabicum introgression lines in hexaploid and tetraploid wheats

Cited by 6 publications

References 45 publications

Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of Saccharum spp.

Cytogenetic Characterization and Metabolomic Differences of Full-Sib Progenies of Saccharum spp.

Development of Specific Thinopyrum Cytogenetic Markers for Wheat-Wheatgrass Hybrids Using Sequencing and qPCR Data

The launch of satellite: DNA repeats as a cytogenetic tool in discovering the chromosomal universe of wild Triticeae

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