Development and multiplexing of microsatellite loci for the near threatened freshwater mussel Potomida littoralis (Cuvier, 1798) using 454 sequencing

Froufe, Elsa; Sobral, Carina; Teixeira, Amílcar; Lopes, Anabela; Sousa, Ronaldo; Varandas, Simone; Lopes‐Lima, Manuel

doi:10.1002/aqc.2335

Cited by 10 publications

(10 citation statements)

References 21 publications

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“…PCR reactions were optimised following Froufe et al (2013): three sets of ten primer pairs each were chosen and initially tested on 52 individuals (four per population) with PCR reactions in simplex to validate selected loci and ascertain optimal annealing temperatures. After these trials, ten loci were selected, combined in two multiplex-PCR reactions (eight in MixA and two in MixB) and tested for and Unio foucauldianus (red symbols) populations from Iberia and Morocco, respectively, included in the present study (Table S1, Supporting information).…”

Section: Microsatellite Development and Analysesmentioning

confidence: 99%

Who lives where? Molecular and morphometric analyses clarify which Unio species (Unionida, Mollusca) inhabit the southwestern Palearctic

et al. 2016

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Many doubts still exist about which freshwater mussel Unio species inhabit Northwest Africa. While some authors refer to the presence of Unio delphinus in the Atlantic North African basins of Morocco, a recent International Union for Conservation of Nature (IUCN) assessment performed on Moroccan Unio species, recognised the existence of a distinct species, Unio foucauldianus, with a critically endangered conservation status. The present study delivered new genetic, morphological, and geographical distribution data on two Unio species (i.e. U. delphinus and U. foucauldianus) greatly increasing the almost non-existent data on these taxa. Bayesian phylogenetic analysis revealed two highly supported geographically concordant clades, which diverged by 3.2 ± 0.6 % (uncorrected p distance): the first distributed across Iberia and corresponding to U. delphinus, and the second distributed across Morocco, corresponding to U. foucauldianus. These results were corroborated by the analysis of ten newly developed microsatellite loci as well as shell morphometry. We suggest that the IUCN critically endangered conservation status of U. foucauldianus should be revised and probably down-listed since its actual distribution is much wider than previously described. Phylogenetic relationships with the other Unio species were resolved, showing that U. delphinus and U. foucauldianus fall inside the pictorum lineage. The estimated molecular rate reported herein (0.265 ± 0.06 % per million years) represents the first for the Unionida and could be used as a reference in future studies.Electronic supplementary material The online version of this article

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Section: Microsatellite Development and Analysesmentioning

confidence: 99%

Who lives where? Molecular and morphometric analyses clarify which Unio species (Unionida, Mollusca) inhabit the southwestern Palearctic

et al. 2016

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“…synonyms following Graf & Cummings (2013) genetic diversity patterns of freshwater bivalve species. Next-generation sequencing has also recently began to be used for both the development of new markers (O'Bryhim et al, 2012;Froufe et al, 2013) and the sequencing of massive genetic resources (e.g. Wang et al, 2012) with multiple applications (e.g.…”

Section: Taxonomy and Systematicsmentioning

confidence: 99%

Biology and conservation of freshwater bivalves: past, present and future perspectives

et al. 2014

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Freshwater bivalves have been highly threatened by human activities, and recently their global decline has been causing conservational and social concern. In this paper, we review the most important research events in freshwater bivalve biology calling attention to the main scientific achievements. A great bias exists in the research effort, with much more information available for bivalve species belonging to the Unionida in comparison to other groups. The same is true for the origin of these studies, since the publishing pattern does not always correspond to the hotspots of biodiversity but is concentrated in the northern hemisphere mainly in North America, Europe and Russia, with regions such as Africa and Southeast Asia being quite understudied. We also summarize information about past, present and future perspectives concerning the most important research topics that include taxonomy, systematics, anatomy, physiology, ecology and conservation of freshwater bivalves. Finally, we introduce the articles published in this Hydrobiologia special issue related with the International Meeting on Biology and Conservation of Freshwater Bivalves held in 2012 in Bragança, Portugal.

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“…sampling in Algeria, to map where the two North African observed haplogroups meet); the use of faster evolving markers to study present connectivity (e.g. Microsatellite markers already developed for Potomida littoralis; Froufe et al, 2013); and assess the male mtDNA genetic structuration for a stronger evolutionary signal.…”

Section: Discussionmentioning

confidence: 99%

Phylogeny, phylogeography, and evolution in the Mediterranean region: News from a freshwater mussel (Potomida, Unionida)

Froufe

Prié

Faria

et al. 2016

Molecular Phylogenetics and Evolution

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a b s t r a c tThe Potomida genus (Bivalvia, Unionida) has a Circum-Mediterranean distribution and like other freshwater mussel species, its populations have suffered dramatic declines. Although this genus is currently considered as monotypic, it has a long history of taxonomic revisions and presently many aspects of its systematics and evolutionary history are unclear. We sampled a total of 323 individuals from 39 different sites across the Potomida genus distribution, and sequenced two mitochondrial (16S rDNA and Cytochrome c Oxidase Subunit I) and one nuclear (28S rDNA) genes to clarify its phylogeny and phylogeographic history. Our results show that the genus includes two well-supported clades, one comprising solely the western Mediterranean species Potomida littoralis, and the other including two eastern Mediterranean species, the Greek endemic P. acarnanica and the Anatolian and Middle Eastern P. semirugata. We suggest that Potomida started radiating during the upper Miocene, and that both vicariance and dispersal events shaped the diversification and distribution of the genus along the Mediterranean region. P. littoralis is further divided in two mitochondrial lineages, one restricted to Europe and the other occurring mostly in North Africa. Moreover, some European basins present both lineages in sympatry. The conservation status of the three recognized species should be reevaluated, particularly P. acarnanica, since it is restricted to two Greek river basins presenting a high risk of extinction. Overall, our results clarify some important gaps in knowledge concerning the phylogeny, phylogeography and evolution of the Potomida genus in the Mediterranean region with important taxonomical, ecological and conservational implications.

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Development and multiplexing of microsatellite loci for the near threatened freshwater mussel Potomida littoralis (Cuvier, 1798) using 454 sequencing

Cited by 10 publications

References 21 publications

Who lives where? Molecular and morphometric analyses clarify which Unio species (Unionida, Mollusca) inhabit the southwestern Palearctic

Who lives where? Molecular and morphometric analyses clarify which Unio species (Unionida, Mollusca) inhabit the southwestern Palearctic

Biology and conservation of freshwater bivalves: past, present and future perspectives

Phylogeny, phylogeography, and evolution in the Mediterranean region: News from a freshwater mussel (Potomida, Unionida)

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