Development and optimisation of an on-line solid phase extraction coupled to ultra-high-performance liquid chromatography–tandem mass spectrometry methodology for the simultaneous determination of endocrine disrupting compounds in wastewater samples

Vega-Morales, T.; Sosa‐Ferrera, Zoraida; Santana‐Rodríguez, José Juan

doi:10.1016/j.chroma.2012.01.077

Cited by 52 publications

(31 citation statements)

References 31 publications

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“…Only 17 α -ethinylestradiol showed a signal enhancement of 12.6%. The results obtained are showed in Table 3 and they are in accordance with those reported in similar studies [19, 24]. …”

Section: Resultssupporting

confidence: 91%

Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry

Guedes-Alonso

Sosa‐Ferrera

Santana‐Rodríguez

2013

Journal of Analytical Methods in Chemistry

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In the last years, hormone consumption has increased exponentially. Because of that, hormone compounds are considered emerging pollutants since several studies have determinted their presence in water influents and effluents of wastewater treatment plants (WWTPs). In this study, a quantitative method for the simultaneous determination of oestrogens (estrone, 17β-estradiol, estriol, 17α-ethinylestradiol, and diethylstilbestrol), androgens (testosterone), and progestogens (norgestrel and megestrol acetate) has been developed to determine these compounds in wastewater samples. Due to the very low concentrations of target compounds in the environment, a solid phase extraction procedure has been optimized and developed to extract and preconcentrate the analytes. Determination and quantification were performed by ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The method developed presents satisfactory limits of detection (between 0.15 and 9.35 ng·L−1), good recoveries (between 73 and 90% for the most of compounds), and low relative standard deviations (under 8.4%). Samples from influents and effluents of two wastewater treatment plants of Gran Canaria (Spain) were analyzed using the proposed method, finding several hormones with concentrations ranged from 5 to 300 ng·L−1.

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“…Only 17 α -ethinylestradiol showed a signal enhancement of 12.6%. The results obtained are showed in Table 3 and they are in accordance with those reported in similar studies [19, 24]. …”

Section: Resultssupporting

confidence: 91%

Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry

Guedes-Alonso

Sosa‐Ferrera

Santana‐Rodríguez

2013

Journal of Analytical Methods in Chemistry

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“…Limits of detection and quantification, in surface water and in tap water, were estimated as the concentrations of each analyte corresponding to a signal-to-noise ratio of 3:1 and 10:1, respectively. Matrix effect has been evaluated by comparison of the peak area of a spiked sample extract (A sp ) with that of a standard solution (A s ), at the same concentration level (250 mg L À 1 for each pollutant), and with the area of the analyte in the unspiked extract (A usp ) [22]. The matrix effect (ME) is expressed as a percentage:…”

Section: Methods Validationmentioning

confidence: 99%

Determination of hormones, a plasticizer, preservatives, perfluoroalkylated compounds, and a flame retardant in water samples by ultrasound-assisted dispersive liquid–liquid microextraction based on the solidification of a floating organic drop

Martín

Santos

Aparicio

et al. 2015

Talanta

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“…To estimate the concentration of each e-EDC, we employed a variety of extraction/purification procedures that have been previously published by our research group [19, 28, 29]. These methods include microwave-assisted extraction (MAE) for sludge samples, ultrasonic-assisted extraction (UAE) for particulate matter, and on-line solid Phase Extraction (SPE) for the dissolved fraction.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Presence of Endocrine-Disrupting Compounds in Dissolved and Solid Wastewater Treatment Plant Samples of Gran Canaria Island (Spain)

Vega-Morales

Sosa‐Ferrera

Santana‐Rodríguez

2013

BioMed Research International

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Liquid and solid samples from two wastewater treatment plants (WWTPs) on Gran Canaria Island (Spain) have been tested for the presence of compounds with endocrine-disrupting properties. The selected degradation stages were sampled bimonthly from each WWTP over the 12-month period from July 2010 to July 2011. The analytical methods used for the determination of the endocrine-disrupting compounds (EDCs) were based on on-line solid phase extraction, microwave-assisted extraction (MAE), and ultrasonic-assisted extraction (UAE) coupled to UHPLC-MS/MS. All of the hyphenated methodologies employed in this work showed good recoveries (72–104%) and sensitivities, with LODs lower than 7.0 ng L−1 and 6.3 ng g−1 for the dissolved and solid fractions, respectively. We have also evaluated the estrogenicity of the samples in terms of their estradiol equivalent concentrations (EEQs). The chemical analysis of the selected EDCs revealed fairly low concentrations for both natural and synthetic oestrogens, alkylphenolic compounds, and bisphenol-A in each of the dissolved, particulate, and sludge samples (ng L−1 or ng g−1). However, the estimated estrogenic activity indicated that the majority of samples could represent an important environmental risk, clearly surpassing the threshold to exert deleterious consequences on living beings.

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Development and optimisation of an on-line solid phase extraction coupled to ultra-high-performance liquid chromatography–tandem mass spectrometry methodology for the simultaneous determination of endocrine disrupting compounds in wastewater samples

Cited by 52 publications

References 31 publications

Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry

Simultaneous Determination of Hormonal Residues in Treated Waters Using Ultrahigh Performance Liquid Chromatography-Tandem Mass Spectrometry

Determination of hormones, a plasticizer, preservatives, perfluoroalkylated compounds, and a flame retardant in water samples by ultrasound-assisted dispersive liquid–liquid microextraction based on the solidification of a floating organic drop

Evaluation of the Presence of Endocrine-Disrupting Compounds in Dissolved and Solid Wastewater Treatment Plant Samples of Gran Canaria Island (Spain)

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