Development and Regulatory Application of Microrna Biomarkers

Krauskopf, Julian; Verheijen, Marcha; Kleinjans, Jos; Kok, Theo M. de; Caiment, Florian

doi:10.2217/bmm.15.50

Cited by 23 publications

(16 citation statements)

References 102 publications

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“…MicroRNAs (miRNAs) are short (20‐25 nucleotides), noncoding RNAs that play imperative roles in various developmental and physiological processes . Previous studies have indicated that tissue miRNA expression profiles can be considered as diagnostic biomarkers for cancer . However, this diagnostic method is limited by its invasiveness and inconvenience .…”

Section: Introductionmentioning

confidence: 99%

Circulating microRNA signatures serve as potential diagnostic biomarkers for Helicobacter pylori infection

Zhang

et al. 2018

J of Cellular Biochemistry

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Helicobacter pylor (H pylori), a Gram‐negative, microaerobic human pathogen, has been found to be involved in many gastroduodenal diseases. Accurate diagnosis of H pylori infection is a vital part of the effective management of gastroduodenal diseases. Circulating microRNAs (miRNAs) have shown the potential to be used as noninvasive biomarkers for the diagnosis of infectious diseases. The aim of this study was to explore plasma miRNAs as noninvasive biomarkers for H pylori infection. We performed a plasma miRNA expression profile using Illumina high‐throughput sequencing and validated the levels of differentially expressed miRNAs in the plasma of 63 H pylori‐infected patients and 41 healthy volunteers by quantitative real‐time polymerase chain reaction (qRT‐PCR). The sequencing results showed that 37 miRNAs were upregulated in the H pylori‐infected patients compared with that in the healthy volunteers, while six miRNAs were downregulated. qRT‐PCR and receiver operator characteristic analysis suggested that the expression of miR‐28‐3p, miR‐143‐3p, miR‐151a‐3p, and miR‐148a‐3p were closely associated with H pylori infection. Therefore, the four plasma miRNA panels mentioned above could serve as promising noninvasive biomarkers of H pylori infection.

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Section: Introductionmentioning

confidence: 99%

Circulating microRNA signatures serve as potential diagnostic biomarkers for Helicobacter pylori infection

Zhang

et al. 2018

J of Cellular Biochemistry

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“…MicroRNAs (miRNAs) are endogenous non-coding RNAs, which play vital roles in tumor development and tumorigenesis (8)(9)(10). In some human cancers, miRNAs are often unregulated and have an oncogenic function, while most miRNAs are downregulated and may possess a tumor-suppressive activity (11)(12)(13).…”

Section: Introductionmentioning

confidence: 99%

miRNA-346 promotes proliferation, migration and invasion in liver cancer

Xia

Duan

et al. 2017

Oncology Letters

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Abstract. Liver cancer primarily accounts for the majority of malignancies of the liver. MicroRNAs (miRNAs) are endogenous non-coding RNAs, which are important in tumorigenesis. Abnormal expression of microRNA-346 (miR-346) has been demonstrated in various types of human cancer, however, its expression and potential molecular mechanism in liver cancer remains to be elucidated. Expression levels of miR-346 in liver cancer cell lines were determined by quantitative polymerase chain reaction. The effect of miR-346 on proliferation was evaluated by an MTT assay; cell migration and invasion were evaluated by Transwell migration and invasion assays and target protein expression was determined by western blotting. The present study observed that miR-346 was upregulated in liver cancer cell lines. miR-346 overexpression promoted cell proliferation, migration and invasion in liver cancer cells and conversely, inhibition of miR-346 resulted in the opposite effects. Furthermore, F-Box and leucine rich repeat protein (FBXL)2 was identified as a direct target of miR-346. miR-346 promoted proliferation, migration and invasion of liver cancer via FBXL2. Overall, these findings demonstrated that miR-346 may act as a potential prognostic marker and therapeutic target against liver cancer in the future.

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“…were generated using Next Generation Sequencing technology, enabling reliable quantification of in particular low‐abundance miRNAs. Circulating miRNAs were found to be stable even under conditions as harsh as boiling, extreme pH, long‐time storage at room temperature, and multiple freeze‐thaw cycles . Details regarding sample collection, sample preparation, sequencing, and data processing can be found in Krauskopf et al ., and see for patient characteristics and levels of liver damage markers.…”

Section: Methodsmentioning

confidence: 99%

Acetaminophen Overdose as a Potential Risk Factor for Parkinson's Disease

Bohler

Liu

Krauskopf

et al. 2019

Clinical Translational Sci

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Four complementary approaches were used to investigate acetaminophen overdose as a risk factor for Parkinson's disease (PD). Circulating microRNAs (miRNAs) serum profiles from acetaminophen-overdosed patients were compared with patients with terminal PD, revealing four shared miRNAs. Similarities were found among molecular structures of dopamine (DA), acetaminophen, and two known PD inducers indicating affinity for dopaminergic transport. Potential interactions between acetaminophen and the human DA transporter were confirmed by molecular docking modeling and binding free energy calculations. Thus, it is plausible that acetaminophen is taken up by the dopaminergic transport system into the substantia nigra (SN). A ChEMBL query identified proteins that are similarly targeted by DA and acetaminophen. Here, we highlight CYP3A4, present in the SN, a predominant metabolizer of acetaminophen into its toxic metabolite N-acetyl-p-benzoquinone imine and shown to be regulated in PD. Overall, based on our results, we hypothesize that overdosing of acetaminophen is a potential risk factor for parkinsonism.Parkinson's disease (PD) affects about 0.5% of the population aged 60-69, 1% aged 70-79, and up to 2% aged above 80 years of age. 1 Familial mutations are responsible for ~ 10% of PD cases, leaving 90% sporadic cases for which causes are poorly understood. The toxicant 1-methyl-4-phenylpyridinium (MPP + ) is taken up by dopamine (DA) transporters (DATs) 2 and known to induce parkinsonism, which implies that exogenous factors may indeed induce PD. In addition, environmental toxicants, including pesticides (e.g., Paraquat), pollutants (metals and organochlorides), as well as dietary contaminants and drugs, such as methamphetamine (METH) have been suggested to cause sporadic PD. 3,4 Acetaminophen is a widely used analgesic/antipyretic agent. Although acetaminophen is relatively safe when used at therapeutic doses, acetaminophen poisonings are quite frequent. In fact, acetaminophen is responsible for >70,000 visits to the hospital and is responsible for around 300-400 deaths/year in the United States alone. 5 Acetaminophen toxicity is also a major issue in acetaminophen-opioid products, which led the US Food and Drug Administration (FDA) to limit the dose of acetaminophen in these combinations to 325 mg/ dose. 5 Hepatocellular injury due to formation of reactive metabolites is considered as a hallmark of acetaminophen toxicity. In rodent studies, acetaminophen has been shown to also cause damage to the brain, although it is not entirely clear

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Development and Regulatory Application of Microrna Biomarkers

Cited by 23 publications

References 102 publications

Circulating microRNA signatures serve as potential diagnostic biomarkers for Helicobacter pylori infection

Circulating microRNA signatures serve as potential diagnostic biomarkers for Helicobacter pylori infection

miRNA-346 promotes proliferation, migration and invasion in liver cancer

Acetaminophen Overdose as a Potential Risk Factor for Parkinson's Disease

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