2013
DOI: 10.1016/j.jpba.2013.07.047
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Development and validation of a UHPLC–MS/MS procedure for quantification of the Pseudomonas Quinolone Signal in bacterial culture after acetylation for characterization of new quorum sensing inhibitors

Abstract: The appearance of antibiotic resistance requires novel therapeutic strategies. One approach is to selectively attenuate bacterial pathogenicity by interfering with bacterial cell-to-cell communication known as quorum sensing. The PQS quorum sensing system of Pseudomonas aeruginosa employs as signal molecule the Pseudomonas Quinolone Signal (PQS; 2-heptyl-3-hydroxy-4-(1H)-quinolone), a key contributor to virulence and biofilm formation. Thus, interference with PQS production is considered as promising approach … Show more

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Cited by 26 publications
(18 citation statements)
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“…Pseudomonas aeruginosa strains were cultivated as previously described (Maurer et al, 2013). After letting PA14 strains grow for 6 h in LB, an aliquot of bacterial culture was centrifuged for 10 min at 4835 × g and 25°C using a Rotina 380R Centrifuge (Hettich, Germany).…”
Section: Methodsmentioning
confidence: 99%
“…Pseudomonas aeruginosa strains were cultivated as previously described (Maurer et al, 2013). After letting PA14 strains grow for 6 h in LB, an aliquot of bacterial culture was centrifuged for 10 min at 4835 × g and 25°C using a Rotina 380R Centrifuge (Hettich, Germany).…”
Section: Methodsmentioning
confidence: 99%
“…Extracellular levels of HHQ were determined according to the method of Lépine et al with the following modifications. 32,33 An aliquot of 500 µl of bacterial cultures were supplemented with 50 µl of a 10 µM methanolic solution of the internal standard (IS) 5,6,7,8-tetradeutero-2-heptyl-4(1H)-quinolone (HHQ-d 4 ) and extracted with 1 ml of ethyl acetate by vigorous shaking. After centrifugation, 400 µl of the organic phase were evaporated to dryness in LC glass vials.…”
Section: Determination Of Extracellular Hhq Levelsmentioning
confidence: 99%
“…The parameters for AHLs analysis were as follows: the nebuliser gas was N 2 ; the flow rate of the dryer gas was set to 800 L/h. The heated capillary and voltage were maintained at 500°C and 0.5 kV, respectively (Maurera et al, 2013). The samples were then passed through a 0.22 lm filter and eluted with ammonium acetate buffer (0.05 M) and acetonitrile at 0.4 mL/min.…”
Section: Signal Molecule Add-back Studiesmentioning
confidence: 99%