Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of Salbutamol Sulphate and Theophylline in Pharmaceutical Dosage Forms

Maithani, Mukesh; Singh, R. M.

doi:10.4172/2155-9872.1000116

Cited by 14 publications

(12 citation statements)

References 12 publications

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“…Degassing is done by degasser techniques like ultra sonication, filtration etc. Ultrasonication Here gas is removed by either bursting of gas bubbles immersing ultra sonic generator into the solvent or by placing the solvent container into ultra sonicator tray [12,13]. This is the commonest technique for degassing of HPLC solvents.…”

Section: Degassingmentioning

confidence: 99%

HPLC: Highly Accessible Instrument in Pharmaceutical Industry for Effective Method Development

Jena¹

2012

Pharm Anal Acta

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The analytical technique of High Performance Liquid Chromatography (HPLC) is used extensively thought the pharmaceutical industry. It allows simultaneously both qualitative and quantitative information of a drug. It is used to provide information on the composition of drug related samples. The information obtained may be qualitative, indicating what compounds are present in the sample. The information obtained may be qualitative, providing the actual amount of compounds in the sample. HPLC is used in all the different stages in the creation of new drug discoveries and also used routinely in the drug manufacturing process. The HPLC techniques is highly accessible to the author's knowledge this review represents the most exhaust description of instrumentation, method development, recent advancement, area of application. This article reviews the most recent advances in sample preparation, separation and the mass spectrometric aspects of high-throughput for method development.

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Section: Degassingmentioning

confidence: 99%

HPLC: Highly Accessible Instrument in Pharmaceutical Industry for Effective Method Development

Jena¹

2012

Pharm Anal Acta

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“…The literature survey reveals that high-performance liquid chromatography (HPLC) and ultraviolet (UV) methods were reported for the estimation of SAL, ETO, and BROM alone or in combination with other drugs in bulk and dosage forms. [4][5][6][7][8][9][10][11][12][13][14] However, so far there is no stability indicating method reported for the same. This initiated an interest to develop a new, simple, and rapid HPLC method of these drugs in combination with the marketed formulation used for the treatment of COPD and asthma.…”

Section: Introductionmentioning

confidence: 99%

Method Development and Force Degradation Studies for Simultaneous Estimation of Salbutamol Sulfate, Etofylline and Bromhexine Hydrochloride in Pharmaceutical Dosage Form Using Reversed-Phase High-Performance Liquid Chromatography Method

Rao¹,

Gawde

2018

Asian J Pharm Clin Res

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Objective: The present study describes the stability indicating reversed-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous estimation of salbutamol sulfate (SAL), etofylline (ETO), and bromhexine hydrochloride (BROM) in pharmaceutical dosage forms.Methods: The proposed RP-HPLC method was developed using Shimadzu prominence-i LC-2030 HPLC system equipped with ultraviolet (UV) detector and chromatographic separation was achieved isocratically using Shim-pack C18 (250 mm×4.6mm, 5 μ) column at a flow rate of 1 ml/min and the run time was 13 min. The mobile phase consisted of acetonitrile: 0.1M potassium dihydrogen phosphate buffer (35:65) with pH adjusted to 3.0 and eluents were scanned using UV detector at 225 nm.Result: The retention time of SAL, ETO, and BROM was found to be 2.319 min, 2.698 min, and 10.329 min, respectively. The calibration curve was linear over the concentration ranges of 1.6–3.2 μg/ml, 160–320 μg/ml, and 6.4–12.8 μg/ml for SAL, ETO, and BROM, respectively.Conclusion: The stability indicating method was developed by subjecting the drugs to stress conditions such as acid and base hydrolysis, oxidation, humidity, and photo- and thermal degradation and the degraded products formed were resolved successfully from the samples. Therefore, the proposed method can be used as a more convenient and efficient option for the simultaneous estimation of all the three drugs in bulk and combined

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“…Many analytical methods including high performance liquid chromatography [ 7 , 8 , 9 ], spectrophotometry [ 10 , 11 , 12 , 13 , 14 ], and capillary electrophoresis [ 15 , 16 ] have been reported for determination of salbutamol sulfate in different samples. However, most of these methods are known to be time consuming, require advanced technical expertise, costly equipment and complex sample pretreatment procedure using organic solvents aggravating environmental pollution.…”

Section: Introductionmentioning

confidence: 99%

Differential pulse voltammetric determination of salbutamol sulfate in syrup pharmaceutical formulation using poly(4-amino-3-hydroxynaphthalene sulfonic acid) modified glassy carbon electrode

Amare

Menkir²

2017

Heliyon

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A new method for determination of salbutamol sulfate has been developed using poly(4-amino-3-hydroxynaphthalene sulfonic acid/GCE. Cyclic voltammetric investigation of the electrochemical behavior of salbutamol sulfate at the polymer modified glassy carbon unveiled electrocatalytic activity of the modifier towards irreversible oxidation of salbutamol sulfate. Dependence of peak current predominantly on scan rate than on square root of scan rate, and peak potential shift with pH demonstrated that oxidation of salbutamol sulfate at the polymer modified electrode follows adsorption reaction kinetics with proton participation.Under optimized solution and differential pulse voltammetric parameters, the oxidative peak current showed linear dependence on salbutamol sulfate concentration in the range 0.2 to 8 μM with method detection limit (3s/m) and determination coefficient (R2) of 6.8 × 10−8 M and 0.99786, respectively. Low method detection limit, relatively wide linear range, and recovery results of spiked standard salbutamol sulfate in syrup samples in the range 96.7–98.9% validated the method for determination of salbutamol sulfate in pharmaceutical formulations.Differential pulse voltammetric analysis of salbutamol sulfate syrup formulation for its salbutamol sulfate content revealed 98.8 to 99.3% of the labeled value confirming the applicability of the developed method for determination of salbutamol sulfate in real samples.

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Development and Validation of a Stability-Indicating HPLC Method for the Simultaneous Determination of Salbutamol Sulphate and Theophylline in Pharmaceutical Dosage Forms

Cited by 14 publications

References 12 publications

HPLC: Highly Accessible Instrument in Pharmaceutical Industry for Effective Method Development

HPLC: Highly Accessible Instrument in Pharmaceutical Industry for Effective Method Development

Method Development and Force Degradation Studies for Simultaneous Estimation of Salbutamol Sulfate, Etofylline and Bromhexine Hydrochloride in Pharmaceutical Dosage Form Using Reversed-Phase High-Performance Liquid Chromatography Method

Differential pulse voltammetric determination of salbutamol sulfate in syrup pharmaceutical formulation using poly(4-amino-3-hydroxynaphthalene sulfonic acid) modified glassy carbon electrode

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