2019
DOI: 10.4269/ajtmh.18-0645
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Development and Validation of a Copro-Enzyme–Linked Immunosorbent Assay Sandwich for Detection of Echinococcus granulosus–Soluble Membrane Antigens in Dogs

Abstract: Cystic echinococcosis (CE) is a parasitic zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus. Detection of the adult stage in the canine definitive host is essential for estimating infection rates, surveillance and monitoring of CE control programs. This study sought to develop and validate a coproantigen sandwich enzyme-linked immunosorbent assay (copro-ELISA), based on antibodies against E. granulosus-soluble membrane antigens (EGMA), that is capable of distinguishing infected and no… Show more

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Cited by 13 publications
(9 citation statements)
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“…In recent years, several coproELISAs have been standardized for use in existing control programmes in endemic areas of the world (Guarnera, Santillan, Botinelli, & Franco, ; Jara et al, ; Morel et al, ; Pierangel et al, ) and they have been included in the surveillance systems of the countries, such as Peru, Argentina, Uruguay, China and Kyrgyzstan (Centro Panamericano Fiebre Aftosa, ; Irabedra et al, ; van Kesteren, Mastin, Torgerson, Mytynova, & Craig, ; Larrieu & Zanini, ).…”
Section: Ce Surveillancementioning
confidence: 99%
See 1 more Smart Citation
“…In recent years, several coproELISAs have been standardized for use in existing control programmes in endemic areas of the world (Guarnera, Santillan, Botinelli, & Franco, ; Jara et al, ; Morel et al, ; Pierangel et al, ) and they have been included in the surveillance systems of the countries, such as Peru, Argentina, Uruguay, China and Kyrgyzstan (Centro Panamericano Fiebre Aftosa, ; Irabedra et al, ; van Kesteren, Mastin, Torgerson, Mytynova, & Craig, ; Larrieu & Zanini, ).…”
Section: Ce Surveillancementioning
confidence: 99%
“…In recent years, several coproELISAs have been standardized for use in existing control programmes in endemic areas of the world (Guarnera, Santillan, Botinelli, & Franco, 2000;Jara et al, 2019;Morel et al, 2013;Pierangel et al, 2010)…”
Section: Surveillance In Dogsmentioning
confidence: 99%
“…Nonetheless, the sensitivity may be improved through the use of new copromicroscopic techniques with higher sensitivity, such as Mini-FLOTAC and FLOTAC, as described by Maurelli et al (2014). Techniques based on the detection of antigens in feces have also been used and have shown a wide range of sensitivity and specificity (Deplazes et al, 1990;Sen et al, 2011;Christy et al, 2012;Shimelis and Tadesse, 2014;Jara et al, 2019). Due to the different sensitivity and specificity presented by both microscopic and antigen detection techniques and with the advent of molecular techniques based on the detection of nucleic acids, certain aspects of sensitivity and specificity were improved (Jacobs et al, 1997;Li et al, 2007;Pinelli et al, 2013;Knapp et al, 2016); however, supplies and equipment are usually expensive and the procedures require human resources with specific training which are not widely available in endemic areas, complicating its implementation.…”
Section: Introductionmentioning
confidence: 99%
“…CoproELISA yielded a better sensitivity but included false positives [ 13 ]. CoproELISA was confirmed as an effective, safe, and easy method of detection in Australia [ 14 ], Argentina [ 15 ], Uruguay [ 16 ], or Peru [ 17 ]. This study provides a reference for improving control measures and assessment of the prevalence of echinococcosis in the endemic counties of China, a key step towards elimination.…”
Section: Discussionmentioning
confidence: 99%