Development and Validation of a Multi-class UHPLC-MS/MS Method for Determination of Antibiotic Residues in Dairy Products

Schwaiger, Barbara; König, Jürgen; Lesueur, Céline

doi:10.1007/s12161-017-1101-1

Cited by 34 publications

(15 citation statements)

References 28 publications

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“…SAs were eluted with 8 mL of ethyl acetate, then evaporated to dryness under 12 psi N 2 flow at 42 • C. Finally, the residue was reconstituted in 0.25 mL of methanol/water (15/85) [43]. The analysis was based on other methods of detection of antibiotics in milk [44,45].…”

Section: Sulfonamides Extraction and Quantification By Uhplc-ms/msmentioning

confidence: 99%

Sulfonamides in Tomato from Commercial Greenhouses Irrigated with Reclaimed Wastewater: Uptake, Translocation and Food Safety

et al. 2021

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The presence of antibiotics in crops is mainly caused by their irrigation with reclaimed wastewater and by the use of organic amendments of animal origin. During this work, the fate of sulfonamide antibiotics in tomato crop has been assessed in two commercial greenhouses located in Almería (Spain) irrigated with reclaimed wastewater. Samplings were made annually for two years. Sulfonamides in several parts of the plant (roots, leaves and fruits) as well as reclaimed wastewater, amendments and soils were analyzed by UHPLC-MS/MS. The results showed that sulfonamides accumulated in soils (sulfamethoxazole between 2 and 14 µg Kg−1; sulfadiazine, sulfathiazole, sulfapyridine, sulfamerazine and sulfadimethoxine in concentrations below 1 µg Kg−1) were in the reclaimed wastewater at concentrations in the ng L−1 range. Their distribution in plants depended on the sulfonamide. The sulfonamides detected in tomato were sulfadiazine, sulfapyridine, sulfamethazole, sulfamethoxazole and sulfadimethoxine. Sulfamethoxazole was the antibiotic with highest concentration in tomato fruit, exceeding 30 µg Kg−1. All sulfonamides were below the Acceptable Daily Intake, however, further studies and legislation are needed to assure food safety.

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Section: Sulfonamides Extraction and Quantification By Uhplc-ms/msmentioning

confidence: 99%

Sulfonamides in Tomato from Commercial Greenhouses Irrigated with Reclaimed Wastewater: Uptake, Translocation and Food Safety

et al. 2021

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“…Officially, residues of antibiotics in milk are detected using a screening test and quantification is performed by high‐performance liquid chromatography or gas chromatography associated with mass spectrometry 7,34,35 . The screening tests are nonspecific, while the chromatographs are specific and quantify the substance; however, they are laborious, use many chemical reagents, require trained personnel and their analytical speed is low 2,36 .…”

Section: Resultsmentioning

confidence: 99%

FTIR spectroscopy with chemometrics for determination of tylosin residues in milk

et al. 2020

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BACKGROUND Contamination of milk by antibiotic residues represents risks to the health of consumers; therefore they should be monitored. The objective of this study was to propose a methodology for the determination of tylosin residues directly in fluid milk based on mid‐infrared spectroscopy associated with chemometrics, using attenuated total reflection Fourier transform infrared (ATR‐FTIR) spectroscopy associated with multilayer perceptron network (MLP) and partial least squares (PLS). RESULTS MLP was shown to be adequate for the discrimination of milk samples contaminated with tylosin below or equal to or above the maximum residue limit (MRL), with an accuracy greater than 99%, using FTIR spectra data. PLS was shown to be appropriate for the prediction of the very low concentrations (0–100 μg L−1) of tylosin residues in milk using FTIR spectra data. PLS models with high correlation coefficients (R > 0.99) were generated. CONCLUSION FTIR with chemometrics proved to be a non‐destructive, efficient and low‐cost method for the investigation and quantification of tylosin residues directly in fluid milk. © 2020 Society of Chemical Industry

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“…The repeatability and reproducibility were measured between 2-13% and 6-16%, respectively. Schwaiger et al [42] developed and validated Multi-Class UHPLC-MS/MS Method as per EC 657/2002 and simultaneous determined 30 substances from different groups of compounds (quinolones, lincosamides, macrolides, diamino-pyrimidine derivates and β-lac + , Tet + , and Sulfa + ) in various kinds of dairy products. Erythromycin A and penicillin G are most problematic substances having high recovery rates and high standard deviations.…”

Section: Lc-msmentioning

confidence: 99%

Assessment of various veterinary drug residues in animal originated food products

Parmar

Chaubey

Gupta³

et al. 2021

Vet World

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The veterinary drugs are broad-spectrum antibacterial antibiotics; it uses to cure the animal disease. Many countries have banned veterinary drug residues like nitrofurans metabolites, chloramphenicol. However, the people were administrated veterinary drugs to animals as illegal to increase the milk production in animals for economic benefit. The results of illegally use of veterinary drugs remain as a residue in animal product like milk and it is very harmful to whom consume it cause cancer and allergic for human being which has entered the concern among milk consumers. To control illegal use of veterinary drugs, the government of India has restricted its use in animals. For the identification and confirmation of veterinary drug residues in animal products, analytical techniques such as liquid chromatography and mass spectrometry are available. These are very sophisticated equipments which are available nowadays and their methodologies for the analytical method validation are described by European commission 2002/657/EC. The use of veterinary drugs is a big challenge to effectively identify and authorization of their use. There are so many analytical techniques are using very effectively and taking very less time to protect the consumers from their adverse effects. These techniques take very less time to identify more groups of compounds such as tetracycline, sulfonamides, anthelmintic, and macrolides in single multi-residue method. These methods having validation parameters include system precision, calibration curve, accuracy, limit of detection, and quantification. Therefore, improvement in the existing technologies and accessibility of new screening methodologies will give opportunities for automation that helps in obtaining the results in very less time and improved sensitivity and specificity which contribute to better safety assurance, standard, and quality of various food products of animal origin.

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Development and Validation of a Multi-class UHPLC-MS/MS Method for Determination of Antibiotic Residues in Dairy Products

Cited by 34 publications

References 28 publications

Sulfonamides in Tomato from Commercial Greenhouses Irrigated with Reclaimed Wastewater: Uptake, Translocation and Food Safety

Sulfonamides in Tomato from Commercial Greenhouses Irrigated with Reclaimed Wastewater: Uptake, Translocation and Food Safety

FTIR spectroscopy with chemometrics for determination of tylosin residues in milk

Assessment of various veterinary drug residues in animal originated food products

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