Development and validation of a High performance liquid chromatography- Mass spectrometry method for 17a-methyltestosterone in Aquatic water systems

Marwah, Ashok; Nianzu, Xu; Marwah, Padma; Barry, Terence P.

doi:10.31018/jans.v2i2.117

Cited by 3 publications

(1 citation statement)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Drying gas temperature and capillary voltage of the system were adjusted at 350 °C and 4000 V, respectively. LC–MS/MS was performed using target ions at m/z 271 for estradiol 23 , 24 , m/z 315 for progesterone 23 , 25 , and m/z 289 for testosterone 26 , respectively.…”

Section: Methodsmentioning

confidence: 99%

Sex steroids and steroidogenesis-related genes in the sea cucumber, Holothuria scabra and their potential role in gonad maturation

Thongbuakaew

Suwansa‐ard

Chaiyamoon

et al. 2021

Sci Rep

View full text Add to dashboard Cite

The sea cucumber Holothuria scabra is an economically valuable marine species which is distributed throughout the Asia–Pacific region. With the natural population declining due to over fishing, aquaculture of this species is deemed necessary. Hence, it is essential to understand the mechanisms regulating the reproduction in order to increase their populations. Sex steroids, including estrogens, androgens and progestogens, play an important role in reproduction in most vertebrates and several invertebrates. It has been proposed that sea cucumbers have the same sex steroids as vertebrates but the steroidogenic pathway in the sea cucumbers is still unclear. In this study, we demonstrated by using liquid chromatography-tandem mass spectrometry (LC–MS/MS) that sex steroids (estradiol, progesterone, and testosterone) were present in H. scabra neural and gonadal tissues. In silico searches of available sea cucumber transcriptome data identified 26 steroidogenesis-related genes. Comparative analysis of encoded proteins for the steroidogenic acute regulatory protein (HscStAR), CYP P450 10, 17 and 3A (HscCYP10, HscCYP17, HscCYP3A) and hydroxysteroid dehydrogenases (Hsc3β-HSD, Hsc17β-HSD) with other species was performed to confirm their evolutionary conservation. Gene expression analyses revealed widespread tissue expression. Real-time PCR analysis revealed that HscStAR, HscCYP10, Hsc3β-HSD, and Hsc17β-HSD gene expressions were similar to those in ovaries and testes, which increased during the gonad maturation. HscCYP17 mRNA was increased during ovarian development and its expression declined at late stages in females but continued high level in males. The expression of the HscCYP3A was high at the early stages of ovarian development, but not at other later stages in ovaries, however it remained low in testes. Moreover, a role for steroids in reproduction was confirmed following the effect of sex steroids on vitellogenin (Vtg) expression in ovary explant culture, showing upregulation of Vtg level. Collectively, this study has confirmed the existence of steroids in an echinoderm, as well as characterizing key genes associated with the steroidogenic pathway. We propose that sex steroids might also be associated with the reproduction of H. scabra, and the identification of biosynthetic genes enables future functional studies to be performed.

show abstract

Section: Methodsmentioning

confidence: 99%

Sex steroids and steroidogenesis-related genes in the sea cucumber, Holothuria scabra and their potential role in gonad maturation

Thongbuakaew

Suwansa‐ard

Chaiyamoon

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

Steroids and genes related to steroid biosynthesis in the female giant freshwater prawn, Macrobrachium rosenbergii

et al. 2016

View full text Add to dashboard Cite

Transformation of 17a-methyltestosterone in aquatic-sediment systems

Barry

Marwah

2011

JANS

Self Cite

View full text Add to dashboard Cite

Abstract:Two sediment samples that differed with respect to total organic carbon and texture ("sand" and "clay") were exposed to radio inert 17α-methyltestosterone (MT) or [14 C]-radiolabeled 17α-methyltestosterone ( 14 C-MT), under both aerobic and anaerobic conditions, for up to 56 days, to characterize the fate of MT in the aquatic environment. Radio inert MT was quantified by a highly sensitive liquid chromatography/mass spectrometry (LC-MS) method and radioactive MT was quantified by HPLC using an in-line flow liquid scintillation counter (LSC). The data suggest that MT entering the aquatic environment is converted into metabolites that become tightly associated with the sediment. Half-lives for MT dissipation in the sediment systems ranged from 2-9 days, depending on the sediment type and the presence of oxygen. Sediment type had little effect on MT dissipation. The mineralization of MT under aerobic conditions was low (<9% conversion of MT to CO 2 ).

show abstract

Development and validation of a High performance liquid chromatography- Mass spectrometry method for 17a-methyltestosterone in Aquatic water systems

Cited by 3 publications

References 18 publications

Sex steroids and steroidogenesis-related genes in the sea cucumber, Holothuria scabra and their potential role in gonad maturation

Sex steroids and steroidogenesis-related genes in the sea cucumber, Holothuria scabra and their potential role in gonad maturation

Steroids and genes related to steroid biosynthesis in the female giant freshwater prawn, Macrobrachium rosenbergii

Transformation of 17a-methyltestosterone in aquatic-sediment systems

Contact Info

Product

Resources

About