Development and Validation of a Micellar Electrokinetic Chromatography Method for Quantitative Determination of Butenolides in Piper malacophyllum (C. Presl) C. DC.

Abstract

A novel high-performance MEKC method developed for the analysis of butenolides 1 and 2 in leaf extracts of P. malacophyllum allowed their quantitative determined within an analysis time shorter than 5 min and the results indicated CE to be a feasible analytical technique for the quantitative determination of butenolides in Piper extracts.

CE and CEC analysis of phytochemicals in herbal medicines

“…Ultrasonic extraction was the most frequently used method due to its efficiency and easy manipulation. Indeed, it was employed in about two‐thirds (180 out of 271 articles) of cited references (Supporting Information Tables 1–7) 4–271. Other conventional extraction methods included reflux 6, 14, 15, 21, 45, 74, 84, 87, 97, 117, 121, 123, 131, 134, 157, 162, 164, 169, 171, 172, 176, 179, 182–184, 205, 212, 227, 235, 236, 259, Soxhlet extraction 23, 89, 91, 128, 131, 244, heating extraction 22, 39, 51...…”

“…The influence of several parameters, including buffer type and concentration, pH, additives, voltage, and temperature, has been discussed in our previous reviews 1, 2. Besides, micelle and microemulsion composition for MEKC and MEEKC (Supporting Information Tables 5 and 6) 187–262, as well as sample matrix and injection parameters for online preconcentration 5, 7, 8, 25, 34, 37, 52, 112, 187, 196–198, 207, 209, 220, 238–240, 244, 247 should also be considered.…”

“…Therefore, triangle and tetrahedron optimization methods based on the interaction of different factors were adapted for the selection of BGE in CE fingerprint development 182, 183. Chemometrics‐based techniques such as factorial design 4, 48, 199, 237, 249, 262 and response surface methodology 160, 217 were also employed. Factorial design of 3 2 and 2 3 was used for the optimization of CE analysis of phenolic acids in exotic fruits 48 and butenolides in Piper malacophyllum 249, respectively.…”

“…Chemometrics‐based techniques such as factorial design 4, 48, 199, 237, 249, 262 and response surface methodology 160, 217 were also employed. Factorial design of 3 2 and 2 3 was used for the optimization of CE analysis of phenolic acids in exotic fruits 48 and butenolides in Piper malacophyllum 249, respectively. Orthogonal design, one kind of fractional factorial design, has been employed for the optimization of CE analysis of alkaloids 4, benzoic acid compounds 199, and iridoid glycosides 237 in herbs.…”

Composição química e avaliação da atividade antimicrobiana do óleo essencial das folhas de Piper malacophyllum (C. Presl.) C. DC.

“…Esta importante e significativa atividade associada aos butenolídeos conduziu ao desenvolvimento de metodologia analítica baseada em eletroforese capilar para a quantificação rápida desses metabólitos em extratos de P. malacophyllum. 12 Neste trabalho, é descrita pela primeira vez a composição química e a atividade antibacteriana, antifúngica e antiprotozoária do óleo essencial obtido a partir das folhas de P. malacophyllum.…”