Development and validation of a UPLC-MS/MS method to quantify fructose in serum and urine

Effects of fructose restriction on blood pressure: Secondary analysis of a double-blind randomized controlled trial

“…Twenty-four-hour urinary fructose levels, used as a biomarker of dietary fructose intake and, hence, adherence [24], showed a very similar pattern (Supplementary Figure 2D). Serum uric acid levels, but not 24 h urinary uric acid levels, showed a borderline significant reduction in the intervention group only (Supplementary Figure 3A-B).…”

“…Insulin resistance was estimated with the homeostasis model assessment of insulin resistance (HOMA2-IR) calculator (www.dtu.ox.ac.uk). A 24-hour urine sample was collected at baseline and at completion in pre-acidified plastic containers for determination of sodium (Indirect ISE-method, Cobas 8000 instrument, Roche Diagnostics, Mannheim, Germany), fructose (Ultra Performance Liquid Chromatography-tandem Mass Spectrometry [24], and uric acid levels (Enzymatic colorimetric assay, Cobas 8000 instrument, Roche Diagnostics, Mannheim, Germany).…”

“…Second, we used an objective, validated biomarker of fructose intake, i.e. urinary fructose levels [24], to demonstrate overall adherence. Finally, we used state-of-the-art markers of endothelial function.…”

Effects of fructose added to an oral glucose tolerance test on plasma glucose excursions in healthy adults

“…Plasma glucose levels were determined every 15 min by using the YSI2300 STAT Plus Glucose Lactate Analyser (YSI, Yellow Springs, OH). Serum fructose concentrations were measured with a recently developed and validated Ultra Performance Liquid Chromatography–tandem Mass Spectrometry method [ 15 ]. Serum lipids were measured by an enzymatic colorimetric assay (Cobas 8000 instrument, Roche Diagnostics, Mannheim, Germany).…”

A Hypothesis From Metabolomics Analysis of Diabetic Retinopathy: Arginine-Creatine Metabolic Pathway May Be a New Treatment Strategy for Diabetic Retinopathy

“…Compared to NMR, MS has a much higher sensitivity and is therefore able to measure a wider range of metabolites ( 40 , 43 , 73 , 74 ). In particular, UPLC offers excellent chromatographic separation, high speed, and high sensitivity, allowing the detection of thousands of metabolites within a short time ( 75 – 78 ). HPLC tandem MS plays a huge contribution in research that requires high throughput, such as natural drug development and disease biomarker identification ( 79 – 83 ).…”