Development and validation of a UFLC–MS/MS method for the determination of anhydrosafflor yellow B in rat plasma and its application to pharmacokinetic study

Yue, Shi‐Jun; Wu, Liang; Qu, Cheng; Tang, Yuping; Jin, Yi; Li, Shujiao; Shen, Juan; Shi, Xu-Qin; Shan, Chenxiao; Cui, Xiao‐Bing; Zhang, Li; Yang, Haijun; Li, Qian; Qian, Dawei; Duan, Jin‐Ao

doi:10.1016/j.jchromb.2015.09.013

Cited by 8 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HSYA, as the major component of Carthamus tinctorius L., is a cause for concern in recent years due to its multiple pharmacological effects in treating cerebral I/R. Hence, it was selected as an effective marker for the quality control of safflower in Chinese Pharmacopoeia ( Yue et al, 2015 ). However, previous research studies on HSYA against I/R injury remained inadequate because of the complexity and diversity of Chinese herbal medicinal ingredients.…”

Section: Discussionmentioning

confidence: 99%

“…As the representational bioactive compound extracted from safflower, hydroxysafflor yellow A (HSYA, Supplementary Figure S1A ) has good therapeutic effects on stroke, such as oxygen free radical scavenging, antiapoptotic, anti-inflammatory, and antioxidative stress properties ( Qi et al, 2014 ). In addition, anhydrosafflor yellow B (AHSYB, Supplementary Figure S1B ), another water-soluble component in safflower, whose content was reported to be half as much as HSYA, also exhibited excellent antioxidative effects ( Yue et al, 2015 ). However, previous reports mainly focus on the neuroprotective effect of HSYA and few articles mentioning about the pharmacological actions of AHSYB in cerebral I/R injury.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hydroxysafflor Yellow A and Anhydrosafflor Yellow B Protect Against Cerebral Ischemia/Reperfusion Injury by Attenuating Oxidative Stress and Apoptosis via the Silent Information Regulator 1 Signaling Pathway

et al. 2021

View full text Add to dashboard Cite

Hydroxysafflor yellow A (HSYA) and anhydrosafflor yellow B (AHSYB) are the main water-soluble compounds in Carthamus tinctorius L. However, studies on the effect of AHSYB on cerebral ischemia/reperfusion (I/R) injury and the therapeutic effect of HSYA by regulating silent information regulator 1 (SIRT1) pathway remain obscure. In this study, we investigated whether the neuroprotective effects of HSYA and AHSYB on oxygen-glucose deprivation/reoxygenation in primary-cultured hippocampal neuronal cells and the middle cerebral artery occlusion and reperfusion model in rats are associated with the regulation of the SIRT1 pathway. In vitro, HSYA and AHSYB increased cell viability, depressed oxidation properties, and reduced neuronal cell apoptosis. In vivo results showed that HSYA and AHSYB effectively reduced infarct volume, improved neurological function, suppressed apoptosis, and decreased the oxidative stress reaction. Besides, RT-PCR and Western blot analysis showed that HSYA and AHSYB increased the mRNA and protein expressions of the main factors in the SIRT1 pathway, including SIRT1, forkhead box O (FOXO) 1, and peroxisome proliferator–activated receptor coactivator 1α (PGC1α), decreased the expression of Bax, and increased the expression of Bcl-2. The results from immunohistochemistry also showed that the expressions of SIRT1, FOXO1, and PGC1α were increased after treatment with HSYA and AHSYB. Furthermore, the neuroprotective effects of HSYA and AHSYB were abolished by EX527 (SIRT1–specific inhibitor). These results indicated that HSYA and AHSYB should be developed into potential drugs for treating cerebral I/R injury via the SIRT1 pathway. Although HSYA and AHSYB have different chemical structures, both of them exert similar neuroprotective properties against I/R injury in vitro and in vivo, which means that AHSYB is also a non-negligible component in safflower.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Hydroxysafflor Yellow A and Anhydrosafflor Yellow B Protect Against Cerebral Ischemia/Reperfusion Injury by Attenuating Oxidative Stress and Apoptosis via the Silent Information Regulator 1 Signaling Pathway

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Therefore, HSYA is extensively used in the field of pharmacology. Anhydrosafflor yellow B (AHSYB) ( Figure 1b) is another important bioactive ingredient [15][16][17] that is isolated from safflower. AHSYB contains numerous hydroxyl groups and possesses strong antioxidative properties; however, there are few studies on AHSYB at present.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Optimization of the Ultrasonic Extraction Method and Determination of the Antioxidant Activities of Hydroxysafflor Yellow A and Anhydrosafflor Yellow B from Safflower Using a Response Surface Methodology

Zhang

Jin

et al. 2020

Molecules

View full text Add to dashboard Cite

An evaluation of the ultrasonic extraction process and the antioxidant activities of hydroxysafflor yellow A (HSYA) and anhydrosafflor yellow B (AHSYB) from safflower are presented herein. Using response surface methodology (RSM), based on a four-factor-three-level Box–Behnken design (BBD), the extraction parameters, namely, temperature, extraction time, solvent-to-material ratio, and extraction power, were optimized for maximizing the yields of HSYA and AHSYB. The maximum yield was obtained at a temperature of 66 °C with an extraction time of 36 min, solvent-to-material ratio of 16 mL/g, and the extraction power of 150 W, which was adjusted according to the actual conditions. The HSYA and AHSYB contents were determined using high performance liquid chromatography (HPLC). The yield and the comprehensive evaluation value of HSYA and AHSYB were calculated. The antioxidant activities of the extracts were determined using a ferric reducing antioxidant power (FRAP) kit and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. The results suggested that the safflower extracts possessed obvious ferric reducing and DPPH radical scavenging activities. The antioxidant activity increased with increasing concentration. The results suggested that optimizing the conditions of ultrasonic extraction using RSM can significantly increase the yields of HSYA and AHSYB from safflower. The safflower extracts showed better antioxidant activity. This study can encourage future research on cardiovascular and cerebrovascular diseases.

show abstract

“…The yellow pigment has lots of ingredients, while hydroxysafflor yellow A (HSYA, Figure 1A) and anhydrosafflor yellow B (AHSYB, Figure 1B) are the dominant ones, which meanwhile provide the main biological function of safflower such as anti‐inflammatory, neuroprotective, and antioxidative effects (Ao, Feng, & Peng, 2018; Chen et al., 2013). Besides HSYA, AHSYB as another important quinochalcone C‐glycoside in safflower, has antiplatelet and anticoagulation activities, it not only plays a direct role in activating blood circulation, but also indirectly contributes to the total bioactivity of safflower series of herb pairs (Qu et al., 2016; Yue et al., 2015). In our pharmacodynamic study of anticerebral ischemia, AHSYB even exerts more significant antioxidative effect than HSYA.…”

Section: Introductionmentioning

confidence: 99%

Response surface optimization of the water immersion extraction and macroporous resin purification processes of anhydrosafflor yellow B from Carthamus tinctorius L.

Zhou

Fangma

Jin

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

In this study,based on a developed high performance liquid chromatographic quantitative method, the suitable extraction and purification conditions of anhydrosafflor yellow B (AHSYB) from safflower were determined by response surface methodology. The optimal water immersion extraction parameters were as follows: liquid to solid ratio of 22:1; extraction temperature of 75°C; extraction time of 35 min. Under these conditions, the maximum extraction yield of AHSYB reached 0.465%. The aqueous extract was further purified by HPD-300 macroporous resin. The optimum adsorption conditions were: pH 2.8; adsorption flow rate of 1.9 mL/min; solution concentration of 0.06 g/mL. The optimum desorption conditions were: ethanol concentrations of 74%; desorption flow rate of 1.6 mL/min; elution volume of 4.4 BV. Under these conditions, the maximum adsorption ratio and desorption ratio reached 1.095 and 0.906 mg/g, respectively. The content of AHSYB reached 6.83%, which was 2.91 times higher than that before purification.

show abstract

Development and validation of a UFLC–MS/MS method for the determination of anhydrosafflor yellow B in rat plasma and its application to pharmacokinetic study

Cited by 8 publications

References 13 publications

Hydroxysafflor Yellow A and Anhydrosafflor Yellow B Protect Against Cerebral Ischemia/Reperfusion Injury by Attenuating Oxidative Stress and Apoptosis via the Silent Information Regulator 1 Signaling Pathway

Hydroxysafflor Yellow A and Anhydrosafflor Yellow B Protect Against Cerebral Ischemia/Reperfusion Injury by Attenuating Oxidative Stress and Apoptosis via the Silent Information Regulator 1 Signaling Pathway

Simultaneous Optimization of the Ultrasonic Extraction Method and Determination of the Antioxidant Activities of Hydroxysafflor Yellow A and Anhydrosafflor Yellow B from Safflower Using a Response Surface Methodology

Response surface optimization of the water immersion extraction and macroporous resin purification processes of anhydrosafflor yellow B from Carthamus tinctorius L.

Contact Info

Product

Resources

About