Development and validation of a high‐performance liquid chromatography method for determination of lisinopril in human plasma by magnetic solid‐phase extraction and pre‐column derivatization

Abstract

A sensitive, reliable and simple HPLC method was developed for the determination of lisinopril in human plasma. The method consists of extraction and clean-up steps based on magnetic solid-phase extraction and pre-column derivatization with a fluorescent reagent. The mobile phase consisted of a mixture of methanol-sodium dihydrogen phosphate (pH 3.0; 0.005 m; 75:25, v/v). The flow rate was set at 0.7 mL/min. Fluorescence detection was performed at 470nm excitation and 530nm emission wavelengths. Total chromatography run time was 5 min. The average extraction recovery of lisinopril and fluvoxamine (internal standard) was ≥82.8%. The limits of detection and quantification were determined as 1 and 3 ng/mL respectively. The method exhibited a linear calibration line over the concentration range of 3-1000 ng/mL with coefficient of determination (r ) of ≥0.98. The within-run and between-run precisions were satisfactory with values of CV of 1.8-12.8% (accuracy from 99.2 to 94.7%) and 2.4-13.7% (accuracy from 99.5 to 92.2%), respectively. These developments led to considerable improvement in method sensitivity and reliability. The method was validated according to the US Food and Drug Administration guidelines. Therefore, it can be considered as a suitable method for determination of lisinopril in plasma samples.

A Comprehensive Review on Developed Pharmaceutical Analysis Methods by Iranian Analysts in 2018

“…Some of the chromatographic studies are performed by HPLC-FD methods. For example: Rastkari et al 123 developed a HPLC-FD method for determination of lisinopril. They used a magnetic polydimethylsiloxane/OHfunctionalized multiwalled carbon nanotubes nanocomposite (PDMS/MWCNT-OH-NC) for extraction of lisinopril from spiked plasma samples and 4-fluoro-7nitro-2,1,3-benzoxadiazole for precolumn derivatization.…”

Determination of Lisinopril in Pure and Tablet form by Using 2-Hydroxynaphthaldehyde as Derivatizing Reagent

“…The literature reveals various analytical methods were described for the determination of LNP such as titrimetric [5,6], Spectrophotometric [7][8][9][10][11][12][13][14][15], spectrofluorometric [16][17][18][19][20], chromatographic [21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38], derivative UV-spectrophotometric, [39][40] polarography [41][42], radioimmunoassay [43] and fluoroimmunoassay [44].…”