2020
DOI: 10.1111/are.14565
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Development and validation of a competitive hybrid ELISA for Seriola lalandi Vitellogenin

Abstract: The main objective of this study was to develop an enzyme-linked immunosorbent assay (ELISA) for Seriola lalandi vitellogenin (Vtg). Nucleotide sequences corresponding to VtgAa, VtgAb and VtgC were obtained from S. lalandi liver transcriptomic data.Two peptides (12 amino acids each) were synthesized according to the predicted conserved regions of VtgAa and VtgAb, and used to generate two polyclonal antibodies in rabbits: anti-S. lalandi VtgA and anti-S. lalandi VtgB. Plasma from male S. lalandi treated with 17… Show more

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