Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances

Gong, Feifei; Hua-xin, Yang; Sun, Wen; Cao, Junzi; Liu, Wanhui

doi:10.1002/elps.201500328

Cited by 7 publications

(3 citation statements)

References 24 publications

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“…Combination of three additives, cationic detergent CTAB, anionic sodium hexanesulfonate, and stereoselector β‐CD, have been employed for MEKC analysis of goserelin and its related substances . The optimized system was composed of 200 mM 6‐aminocaproic acid buffer, pH 4.2, 175 mM CTAB, 20 mM sodium hexanesulfonate, and 3% m/v β‐CD, and was able to separate GSH and its eight analogs within ca 30 min in 50 μm id capillary, 48.5/40 cm total/effective length, at –10 kV separation voltage, 20°C and 220 nm detection wavelength.…”

Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Separations By the Particular Ce And Cec Methodsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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“…An alternative approach for detecting protein prenylation inside mammalian systems that is not reliant on membrane localization as proxy endpoint, is called Protein Lipidation Quantification (PLQ). This method is derived from an established method of Micellar Electro-Kinetic Chromatography (MEKC) that allows selective partition of prenylated proteins into detergent micelles (using SDS) during capillary electrophoresis ( 169 , 170 ). Detergent micelles serve as pseudo-stationary phase and exhibit differential electrophoretic migration compared to unprenylated free analyte.…”

Section: Techniques To Investigate Prenylated Proteomementioning

confidence: 99%

Updates on protein-prenylation and associated inherited retinopathies

Ashok,

Ramachandra Rao

2024

Front. Ophthalmol.

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Membrane-anchored proteins play critical roles in cell signaling, cellular architecture, and membrane biology. Hydrophilic proteins are post-translationally modified by a diverse range of lipid molecules such as phospholipids, glycosylphosphatidylinositol, and isoprenes, which allows their partition and anchorage to the cell membrane. In this review article, we discuss the biochemical basis of isoprenoid synthesis, the mechanisms of isoprene conjugation to proteins, and the functions of prenylated proteins in the neural retina. Recent discovery of novel prenyltransferases, prenylated protein chaperones, non-canonical prenylation-target motifs, and reversible prenylation is expected to increase the number of inherited systemic and blinding diseases with aberrant protein prenylation. Recent important investigations have also demonstrated the role of several unexpected regulators (such as protein charge, sequence/protein-chaperone interaction, light exposure history) in the photoreceptor trafficking of prenylated proteins. Technical advances in the investigation of the prenylated proteome and its application in vision research are discussed. Clinical updates and technical insights into known and putative prenylation-associated retinopathies are provided herein. Characterization of non-canonical prenylation mechanisms in the retina and retina-specific prenylated proteome is fundamental to the understanding of the pathogenesis of protein prenylation-associated inherited blinding disorders.

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“…Electrokinetic chromatography (EKC) has been widely applied in separation science . With micelles as pseudostationary phase (PSP), the EKC separation is based on electrophoresis combined with chromatography, which makes it possible to separate neutral and charged analytes simultaneously.…”

Section: Introductionmentioning

confidence: 99%

Comparison of a polymeric pseudostationary phase in EKC with ODS stationary phase in RP‐HPLC

Zhang

Xing

et al. 2017

Electrophoresis

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Poly(stearyl methacrylate-co-methacrylic acid) (P(SMA-co-MAA)) was induced as pseudostationary phase (PSP) in electrokinetic chromatography (EKC). The n-octadecyl groups in SMA were the same as that in octadecylsilane (ODS) C18 column. Thus, the present work focused on the comparison of selectivity between polymeric PSP and ODS stationary phase (SP), and the effect of organic modifiers on the selectivity of polymeric PSP and ODS SP. 1-butanol could directly interacted with PSP as a Class I modifier, and improved both of the methylene selectivity and polar group selectivity. When the analysis times were similar, the polymeric PSP exhibited better methylene selectivity and polar group selectivity. Although the hydrophobic groups were similar, the substituted benzenes elution order was different between polymeric PSP and ODS SP. Linear solvation energy relationships (LSER) model analysis found that polymeric PSP and ODS SP exhibited two same key factors in selectivity: hydrophobic interaction and hydrogen bonding acidity. But polymeric PSP exhibited relatively strong n- and π-electrons interaction to the analytes.

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Development and validation of a micellar electrokinetic capillary chromatography method for the determination of goserelin and related substances

Cited by 7 publications

References 24 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Updates on protein-prenylation and associated inherited retinopathies

Comparison of a polymeric pseudostationary phase in EKC with ODS stationary phase in RP‐HPLC

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