Development and validation of a liquid chromatography with mass spectrometry method to determine resveratrol and piceid isomers in beeswax

Abstract

This paper represents the first report of a liquid chromatography coupled to electrospray ionization mass spectrometry method for simultaneously analyzing resveratrol and piceid isomers (cis and trans) in beeswax. An efficient extraction procedure has been proposed (average analyte recoveries were between 89 and 95%); this involved a solid-liquid extraction using a mixture of ethanol and water (80:20, v/v) and a concentration step in a rotary evaporator. The separation of all the compounds was achieved using a C18 column and a mobile phase composed of ammonium formate 0.03 M in water and acetonitrile in gradient elution mode at a flow rate of 1 mL/min. The method was fully validated in terms of selectivity, limits of detection and quantification, linearity, precision, and accuracy. The limits of detection and quantification ranged from 1.0 to 1.7 and 3.5 to 5.5 μg/kg, respectively. Finally, the proposed method was applied to analyze beeswax samples collected from experimental and organic apiaries.

Sequential ultrasonic extraction of a Chinese coal and characterization of nitrogen‐containing compounds in the extracts using high‐performance liquid chromatography with mass spectrometry

“…The difficulty in characterization of isomers by MS is discriminating ions with the same m/z , but can be resolved by pre‐separation through HPLC. HPLC coupled with ESI‐MS realized the separation and identification of resveratrol and piceid isomers ( cis and trans ) in beeswax . Therefore, it is necessary to effectively separate and characterize isomers of nitrogen‐containing compounds in coal using HPLC–ESI‐TOF‐MS.…”

Analysis of Intact Glucosinolates in Beeswax by Liquid Chromatography Tandem Mass Spectrometry

“…Nevertheless, the preferable and simplest choice is a solvent extraction with heated water or mixtures of methanol and water (Ares et al 2014;Bell et al 2015;Domínguez-Perles et al 2010;Glauser et al 2012;López-Berenguer et al 2008;Millán et al 2009;Rochfort et al 2008). Consequently, taking into account our experience and the favorable results obtained in recent research (Ares et al 2015;Yáñez et al 2014), we decided to use solvent extraction as the sample treatment. Analysis of intact GLSs was usually performed by LC with ultraviolet or diode array (Ares et al 2014;Gratacós-Cubarsí et al 2010;López-Berenguer et al 2008;Rochfort et al 2006;Truchado et al 2010;) and mass spectrometry (MS) (Alarcón-Flores et al 2013;Ares et al 2014;Bell et al 2015;Domínguez-Perles et al 2010;Glauser et al 2012;López-Berenguer et al 2008;Millán et al 2009;Rochfort et al 2008;Sasaki et al 2012) detectors, while C 18 analytical columns were used in most cases.…”

“…In view of the absence of specific procedures in which intact GLSs were determined in beeswax, it was decided that a solvent extraction should be developed and optimized as the sample treatment; this decision was the result of the simplicity of this extraction procedure and our experience with it (Ares et al 2015;Yáñez et al 2014). Firstly, consideration was given to the amount of beeswax to be analyzed.…”

Bioactive Compounds Contained in Mediterranean Diet and Their Effects on Neurodegenerative Diseases

“…However, the different technological and agronomical processes applied to berries and the slight content on resveratrol, are limiting factors for considering berries as remarkable source of that bioactive compound. Similarly, other foods containing minor amounts of resveratrol are dark cocoa, beer, and bee wax from honeycomb [33][34][35][36][37][38][39].…”