Development and validation of a sample stabilization strategy and a UPLC–MS/MS method for the simultaneous quantitation of acetylcholine (ACh), histamine (HA), and its metabolites in rat cerebrospinal fluid (CSF)

Zhang, Yanhua; Tingley, F. David; Tseng, Elaine; Tella, Max; Yang, Xin; Groeber, Elizabeth; Liu, Jianhua; Li, Wenlin; Schmidt, Christopher J.; Steenwyk, Rick C.

doi:10.1016/j.jchromb.2011.05.030

Cited by 36 publications

(14 citation statements)

References 40 publications

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“…The analysis of CSF samples employing pre‐column derivatization and a generic RPLC method (A: 0.1% formic acid in water/B: 0.1% formic acid in acetonitrile), yielded data on 1‐mHA with levels ~20‐fold higher than what was reported previously on non‐derivatized 1‐mHA . It appears that there was a contribution to the SRM/MS channel of 1‐mHA from an analyte which could not be separated by the generic acidic mobile phase.…”

Section: Resultsmentioning

confidence: 81%

“…Techniques for the analysis of HA and its metabolites include gas chromatography, capillary electrophoresis, and LC employing hydrophilic interaction LC (HILIC) without pre‐column derivatization or reversed‐phase LC (RPLC) with pre‐column derivatization . However, no systematic approach has been reported on the separation and analysis of HA and its metabolite 1‐mHA, including its stereoisomer 3‐mHA, in CSF in the presence of other potentially interfering compounds.…”

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confidence: 99%

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Addressing the need for biomarker liquid chromatography/mass spectrometry assays: A protocol for effective method development for the bioanalysis of endogenous compounds in cerebrospinal fluid

Benitex

McNaney

Luchetti

et al. 2013

Rapid Comm Mass Spectrometry

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Route 206 and Province Line Road, Lawrenceville, NJ 08543, USA RATIONALE: Research on disorders of the central nervous system (CNS) has shown that an imbalance in the levels of specific endogenous neurotransmitters may underlie certain CNS diseases. These alterations in neurotransmitter levels may provide insight into pathophysiology, but can also serve as disease and pharmacodynamic biomarkers. To measure these potential biomarkers in vivo, the relevant sample matrix is cerebrospinal fluid (CSF), which is in equilibrium with the brain's interstitial fluid and circulates through the ventricular system of the brain and spinal cord. Accurate analysis of these potential biomarkers can be challenging due to low CSF sample volume, low analyte levels, and potential interferences from other endogenous compounds. METHODS: A protocol has been established for effective method development of bioanalytical assays for endogenous compounds in CSF. Database searches and standard-addition experiments are employed to qualify sample preparation and specificity of the detection thus evaluating accuracy and precision. RESULTS: This protocol was applied to the study of the histaminergic neurotransmitter system and the analysis of histamine and its metabolite 1-methylhistamine in rat CSF. CONCLUSIONS: The protocol resulted in a specific and sensitive novel method utilizing pre-column derivatization ultra high performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS), which is also capable of separating an endogenous interfering compound, identified as taurine, from the analytes of interest.

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Section: Resultsmentioning

confidence: 81%

mentioning

confidence: 99%

Addressing the need for biomarker liquid chromatography/mass spectrometry assays: A protocol for effective method development for the bioanalysis of endogenous compounds in cerebrospinal fluid

Benitex

McNaney

Luchetti

et al. 2013

Rapid Comm Mass Spectrometry

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“…3,[7][8][9][10][11][12][13] However, differ somatic cell, differ the value of histamine. As we all known, histamine almost existed in mast cells and basophils, the content of that in other somatic cell was low relatively.…”

Section: Introductionmentioning

confidence: 99%

Development of Highly Sensitive Analysis Method for Histamine and Metabolites in Pregnant Women’s Fingernail by UPLC-ESI-MS

Uchibe

Shi

et al. 2018

ANAL. SCI.

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In this study, a highly sensitive analysis method for the rapid detection of histamine (HA), imidazole-4-acetic acid (IAA) and 1-methylhistamine (MHA) in pregnant women fingernails was developed by the ultra-performance liquid chromatography ( coefficient were found to be above 0.9998, the measured detection limit for derivatized histamine and metabolites ranged from 0.06 fmol to 0.15 fmol, and the relative standard derivations of intra-day and inter-day assay were 6.3%. Furthermore, the mean recoveries (%) of the standards added to human fingernails were in the range of 90.2-100.5%. The validatedmethod was successfully applied to analyze human fingernail samples from 3 pregnant women and 3 healthy women. The report about the detection of histamine and metabolites in the fingernails in pregnant women fingernails was the first published.

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“…Due to HA low concentrations in these different fluids even after an important release, sensitive quantification methods are needed. Various analytical methods have already been developed to identify and quantify HA in biological samples: liquid chromatography (LC) [9][10], gas chromatography (GC) [11][12], capillary electrophoresis (CE) [13] coupled with different detection modes. Derivatization was usually used to improve the sensitivity, but was time-consuming, and increased the risk of low analyte recovery.…”

Section: Introductionmentioning

confidence: 99%

Histamine quantification in human plasma using high resolution accurate mass LC–MS technology

Laurichesse

Gicquel

Moreau

et al. 2016

Clinical Biochemistry

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Tables, and 27 References A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPTABSTRACT Background: Histamine (HA) is a small amine playing an important role in anaphylactic reactions. In order to identify and quantify HA in plasma matrix, different methods have been developed but present several disadvantages. Here, we developed an alternative method using liquid chromatography coupled with an ultra-high resolution and accurate mass instrument, Q Exactive TM (Thermofisher) (LCHRMS). Methods:The method includes a protein precipitation of plasma samples spiked with HA-d4as internal standard (IS). LC separation was performed on a C18 Accucore column (100*2.1 mm, 2.6 μm) using a mobile phase containing nonafluoropentanoic acid (3 nM) and acetonitrile with 0.1% (v/v) formic acid on gradient mode. Separation of analytes was obtained within 10 min. Analysis was performed from full scan mode and targeted MS2 mode using a 5 ppm mass window. Ion transition monitored for targeted MS2 mode were 112.0869>95.0607m/z for HA and 116.1120>99.0855m/z for HAd4. Calibration curves were obtained by adding standard calibration dilution at 1 to 180 nM in TrisBSA.Results: Elution of HA and IS occurred at 4.1 min. The method was validated over a range of concentrations from 1 nM to 100 nM. The intra-and inter-run precisions were <15% for quality controls. Human plasma samples from 30 patients were analysed by LCHRMS, and the results were highly correlated with those obtained using the gold standard Radioimmunoassay (RIA) method. Conclusion:Overall, we demonstrate here that LCHRMS is a sensitive method for histamine quantification in biological human plasmas, suitable for routinely use in medical laboratories. In addition, LCHRMS is less time-consuming than RIA, avoid the use of radioactivity, and could then be considered as an alternative quantitative method.

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Development and validation of a sample stabilization strategy and a UPLC–MS/MS method for the simultaneous quantitation of acetylcholine (ACh), histamine (HA), and its metabolites in rat cerebrospinal fluid (CSF)

Cited by 36 publications

References 40 publications

Addressing the need for biomarker liquid chromatography/mass spectrometry assays: A protocol for effective method development for the bioanalysis of endogenous compounds in cerebrospinal fluid

Addressing the need for biomarker liquid chromatography/mass spectrometry assays: A protocol for effective method development for the bioanalysis of endogenous compounds in cerebrospinal fluid

Development of Highly Sensitive Analysis Method for Histamine and Metabolites in Pregnant Women’s Fingernail by UPLC-ESI-MS

Histamine quantification in human plasma using high resolution accurate mass LC–MS technology

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