Development and validation of a multi-residue method for the determination of pesticides in honeybees using acetonitrile-based extraction and gas chromatography–tandem quadrupole mass spectrometry

Walorczyk, Stanisław; Gnusowski, B.

doi:10.1016/j.chroma.2009.07.045

Cited by 120 publications

(54 citation statements)

References 34 publications

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“…Pesticide residues were quantified in the recovered foragers as well as in the flowers of the corn, cotton, soybean, and sorghum using liquid chromatographymass spectrometry (LC-MS) (Barnett et al 2007;Walorczyk and Gnusowski 2009). All the chemical analyses for pesticide residue detection were processed at the USDA National Scientific Laboratories in Gastonia, NC.…”

Section: Detection Of Pesticide Residuesmentioning

confidence: 99%

Landscape and pesticide effects on honey bees: forager survival and expression of acetylcholinesterase and brain oxidative genes

et al. 2017

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-The aim of the present work was to assess the effects of landscape and pesticides on honey bee survival and physiological stress. Integrated use of acetylcholinesterase and detoxification enzymes was tested on honey bee brains for detecting possible exposure to pesticides. Foragers were tracked in agricultural and non-agricultural landscapes in West Tennessee (USA) and then recovered for molecular and chemical analyses. In addition, four honey bee cohorts were fed imidacloprid in the laboratory ad libitum for several weeks and were analyzed by RTqPCR for gene expression. Pesticides were identified at different concentrations in both crop flowers and recovered foragers. No significant differences in foragers' mortality were found among locations. Acetylcholinesterase and detoxification genes showed no response to exposure to pesticides except for GstS3 and GstS4. Our results suggest that none of the studied genes make suitable biomarkers for honey bee exposed to pesticides.honey bee foragers / agricultural landscape / crops / gene expression

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Section: Detection Of Pesticide Residuesmentioning

confidence: 99%

Landscape and pesticide effects on honey bees: forager survival and expression of acetylcholinesterase and brain oxidative genes

et al. 2017

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“…Los experimentos anteriores se realizaron a una concentración de ácido fórmico del 1% (V/V), seleccionada con fundamento en algunos reportes en la literatura (Walorczyk y Gnusowski, 2009). …”

Section: Evaluación De La Concentración De áCido Fórmicounclassified

Desarrollo de una metodología para el análisis de residuos de plaguicidas en leches mediante cromatografía líquida acoplada a espectrometría de masas

et al. 2012

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ResumenSe desarrolló un método multirresiduo para el análisis de plaguicidas en leche mediante el empleo de cromatografía líquida acoplada a espectrometría de masas. Se estudiaron diferentes variaciones al método de extracción QuEChERS utilizando siete diferentes fases extractantes, dos sales amortiguadoras, tres concentraciones de ácido fórmico en la fase extractante y cuatro tiempos de ultrasonido. De los 58 plaguicidas analizados solo tiociclam y tiodicarb presentaron porcentajes de recuperación inferiores al 70%. Los mejores porcentajes de recuperación y menores coeficientes de variación se obtuvieron empleando el buffer de citrato y acetonitrilo con ácido fórmico al 0,5% como extractante. Por otro lado, no se observaron diferencias estadísticas (P > 0,05) a los tiempos de ultrasonido evaluados. Finalmente, se encontró que el método aplicado es preciso y exacto para Palabras clave: alimento, leche, pesticidas, QuEChERS. AbstractIt was developed a multi-residue method for determining pesticides of different physicochemical characteristics presents on milk. The extraction process is based on the QuEChERS method and determination of the compounds was performed using ultra performance liquid chromatography coupled to mass spectrometry. Also, were evaluated seven different phases of extraction, the effect of different times of ultrasound and three concentrations of formic acid on the extraction process. The repeatability of the method results indicated that for most of the compounds showed coefficients of variation below 10%. Furthermore, it was found that there were no statistical differences (P> 0.05) at different times of ultrasound evaluation. Finally, it was found that the developed method is precise and accurate for the analysis of pesticide residues from different chemical groups such as azoles, organophosphorus, benzimidazoles, carbamates, among others.

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“…The original research works devoted to OP pesticides analysis applying various chromatographic techniques: gas chromatography-mass spectrometry (Baugros et al, 2008;Chen & Huang, 2006;Cortés-Aguado et al, 2008;Cunha et al, 2009;Filho et al, 2010aFilho et al, , 2010bHassan et al, 2010;Kolberg et al, 2011;Lavagnini et al, 2011;Lesueur et al, 2008aLesueur et al, , 2008bLi et al, 2011b;Libin et al, 2006;López-Feria et al, 2009;Nguyen et al, 2008;Nguyen et al, 2010;Pang et al, 2006;Rodrigues et al, 2010;Silva et al, 2008;Sinha et al, 2006;Toledano et al, 2010;Wang et al, 2007;Wang et al, 2011;Wu et al, 2011;Yang et al, 2011), gas chromatographytandem mass spectrometry (Barco-Bonilla et al, 2010;Camino-Sánchez et al, 2008;Frenich et al, 2006;Fuentes et al, 2008Fuentes et al, , 2009García-Rodríguez et al, 2010;Lee et al, 2008;Qu et al, 2010;Walorczyk, 2008;Walorczyk & Gnusowski, 2009), gas chromatography-ion trap mass spectrometry (González-Rodríguez et al, 2008;Przybylski & Hommet, 2008), gas chromatography time-of-flight mass spectrometry (Hernández et al, 2010;Portolés et al, 2011), high performance liquid chromatography (Akkad et al, 2010;…”

Section: Chromatographic Methods For Op Pesticides Analysismentioning

confidence: 99%

Organophosphorus Pesticides Analysis

Stoytcheva¹,

Zlatev²

2011

Pesticides in the Modern World - Trends in Pesticides Analysis

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Development and validation of a multi-residue method for the determination of pesticides in honeybees using acetonitrile-based extraction and gas chromatography–tandem quadrupole mass spectrometry

Cited by 120 publications

References 34 publications

Landscape and pesticide effects on honey bees: forager survival and expression of acetylcholinesterase and brain oxidative genes

Landscape and pesticide effects on honey bees: forager survival and expression of acetylcholinesterase and brain oxidative genes

Desarrollo de una metodología para el análisis de residuos de plaguicidas en leches mediante cromatografía líquida acoplada a espectrometría de masas

Organophosphorus Pesticides Analysis

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