Development and validation of a sensitive enzyme immunoassay (EIA) for blood plasma cortisol in female cattle, buffaloes, and goats

Yadav, Rajendra; Mohan, K. S.; Kumar, Vijay; Sarkar, Mihir; Nitu, K.; Meyer, Heinrich; Prakash, B.S.

doi:10.1016/j.domaniend.2013.05.003

Cited by 17 publications

(9 citation statements)

References 18 publications

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“…Because HA concentrations between 1 and 20nM could be arduous to interpret for clinicians [20], a focus was made on samples displaying concentrations measured by RIA comprised in this range (n=47), and a Pearson's correlation coefficient of 0.88 (95% CI 0.80-0.93; p<0.0001) was calculated ( Figure 1C). The subsequent Bland-Altman plot showed an estimated bias of +2.97nM (SD: 3.08nM; 95% limits of agreement: -3.07 -9.01nM) ( In conclusion, besides a slight overestimation of HA concentrations, all these observations support the hypothesis that EIA can be a favorable alternative to RIA, as described previously for other molecules like progesterone, cortisol, or infliximab [21][22][23].…”

Section: High Resolution Accurate Mass Lc-ms Technologysupporting

confidence: 83%

Comparison of two enzymatic immunoassays, high resolution mass spectrometry method and radioimmunoassay for the quantification of human plasma histamine

Poli

Laurichesse

Rostan

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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Histamine (HA) is one of the main immediate mediators involved in allergic reactions. HA plasma concentration is well correlated with the severity of vascular and respiratory signs of anaphylaxis. Consequently, plasma quantification of HA is useful to comfort the diagnosis of anaphylaxis. Currently, radioimmunoassay (RIA) is the gold standard method to quantify HA due to its high sensitivity, but it is time consuming, implicates specific formations and cautions for technicians, and produces hazardous radioactive wastes. The aim of this study was to compare two enzymatic immunoassays (EIA) and one in-house liquid chromatography high-resolution mass spectrometry method (LC-HRMS) with the gold standard method for HA quantification in plasma samples of patients suspected of anaphylaxis reactions. Ninety-two plasma samples were tested with the 4 methods (RIA, 2 EIA and LC-HRMS) for HA quantification. Fifty-eight samples displayed HA concentrations above the positive cut-off of 10nM evaluated by RIA, including 18 highly positive samples (>100 nM). This study shows that Immunotech(®) EIA and LC-HRMS concentrations were highly correlated with RIA values, in particular for samples with a HA concentration around the positive cut-off. In our hands, plasma concentrations obtained with the Demeditec Diagnostics(®) EIA correlated less with results obtained by RIA, and an underestimation of plasma HA levels led to a lack of sensitivity. In conclusion, this study demonstrates that Immunotech(®) EIA and LC-HRMS method could be used instead of RIA to assess plasma HA in human diagnostic use.

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Section: High Resolution Accurate Mass Lc-ms Technologysupporting

confidence: 83%

Comparison of two enzymatic immunoassays, high resolution mass spectrometry method and radioimmunoassay for the quantification of human plasma histamine

Poli

Laurichesse

Rostan

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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“…The validation techniques used in this study for FGM analysis have been previously used in other studies (Bashaw et al, 2016;Chinnadurai et al, 2009;Morrow, Kolver, Verkerk, & Matthews, 2002;Yadav et al, 2013), but obtaining a biological validation (i.e., an ACTH challenge) for this study was not possible. We validated the assay biochemically for this species and monitored variability between assays using quality high and low controls.…”

Section: Fecal Hormone Extraction and Assay Analysesmentioning

confidence: 99%

Monitoring the behavioral and adrenal activity of giraffe (Giraffa camelopardalis) to assess welfare during seasonal housing changes.

Razal¹,

Bryant²,

Miller³

2017

AB&C

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Citation -Razal, C. B., Bryant, J., & Miller, L. J. (2017). Monitoring the behavioral and adrenal activity of giraffe (Giraffa camelopardalis) to assess welfare during seasonal housing changes. Animal Behavior and Cognition, 4(2), 154-164. https://doi.org/10.12966/abc.03.05.2017Abstract -In inclement weather, northern zoos are required to provide animals from warmer climates with indoor exhibits. These indoor exhibits are typically smaller, lack natural substrate, and have lower levels of stimulation for the animals. The purpose of this study was to examine the welfare of four reticulated giraffe (Giraffa camelopardalis reticulata) exhibited at the Chicago Zoological Society -Brookfield Zoo -during the summer in an outdoor enclosure compared to the winter in an indoor enclosure. A combination of direct behavioral observations, percentage of time spent recumbent, and adrenal hormone monitoring through fecal samples were utilized for a more comprehensive look at welfare. Individual variation was observed between the giraffe. An adult female giraffe engaged in Active Forage behavior significantly more in the winter compared to summer (mean difference = 0.23, p = 0.049). An adult female and a juvenile male displayed significantly more Active Non-Forage behavior in the summer than in the winter (mean difference = 0.32, p = 0.048; mean difference = 0.31, p = 0.048). The predominant behavior in the summer for the group as a whole was Active Non-Forage (59%), whereas Active Forage was most prevalent in the winter (60%). There was also a significant positive correlation between time spent recumbent per hour for each 24-hour day measured in the summer and winter (r = 0.51, p = 0.010). Although no significant differences were found for individual FGM concentrations between the seasons, average FGM concentrations for the group were 2306.67 ng/g in the summer and 4261.64 ng/g in the winter. Information gained from this study can help aid animal managers to make informed decisions to ensure each individual giraffe is thriving year-round. In addition, we hope this study can serve as a model to examine the seasonal welfare of other animals in zoological institutions with similar conditions.

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“…Many analytical methods have been proposed for the detection of steroid hormones in biological specimens. Although immunoassays have routinely been used to measure steroid hormones in clinical applications because of the method's simplicity and sensitivity, limitations of the method including poor specificity and the limited dynamic range. Due to the low volatilities of hormones, the general application of gas chromatography/mass spectrometry (GC/MS)‐based analysis was hampered by the time‐consuming derivatization step .…”

Section: Introductionmentioning

confidence: 99%

Determination of hormones in human urine by ultra‐high‐performance liquid chromatography/triple‐quadrupole mass spectrometry

Zhou

Cai

2019

Rapid Comm Mass Spectrometry

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Steroid hormones play a critical role in maintaining the homeostasis of human metabolism. Urine as a noninvasive sample has been extensively used in clinical diagnosis for hormones homeostasis. In this study, the simultaneous characterization of fourteen hormones in urine was performed based on ultra‐high‐performance liquid chromatography/electrospray ionization tandem mass spectrometry (UPHLC/ESI(+)‐MS/MS) with multiple reaction monitoring in the positive ionization mode. The target hormones were cortisone, cortisol, 11‐deoxycortisol, aldosterone, corticosterone, 11‐deoxycorticosterone, progesterone, 17‐OH‐progesterone, pregnenolone, estrone, estradiol, estriol, testosterone and dehydreopiandrosterone. β‐Glucuronidase/sulfatase deconjugation and liquid–liquid extraction (LLE) were conducted for the determination of urinary hormones (free + conjugated forms). The limits of detection (LODs) ranged from 0.2 ng/mL (11‐deoxycortisol and testosterone) to 1 ng/mL (cortisone). The extraction recovery of the targeted compounds ranged from 87% to 127%, indicating sufficient extraction efficiency for the LLE process. Intraday precision was below 10% and the accuracy ranged from 84% to 122%. The profiling analysis of hormones in urine samples helps to understand the metabolic state of biological systems and can be employed as a diagnostic tool in diseases developed by endocrine‐disrupted systems.

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Development and validation of a sensitive enzyme immunoassay (EIA) for blood plasma cortisol in female cattle, buffaloes, and goats

Cited by 17 publications

References 18 publications

Comparison of two enzymatic immunoassays, high resolution mass spectrometry method and radioimmunoassay for the quantification of human plasma histamine

Comparison of two enzymatic immunoassays, high resolution mass spectrometry method and radioimmunoassay for the quantification of human plasma histamine

Monitoring the behavioral and adrenal activity of giraffe (Giraffa camelopardalis) to assess welfare during seasonal housing changes.

Determination of hormones in human urine by ultra‐high‐performance liquid chromatography/triple‐quadrupole mass spectrometry

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