Development and validation of a sensitive LC-MS/MS method for simultaneous quantification of thirteen steroid hormones in human serum and its application to the study of type 2 diabetes mellitus

Liu, Wuwei; Yuan, Daoyi; Han, Minlu; Huang, Jingwen; Xie, Yunying

doi:10.1016/j.jpba.2021.114059

Cited by 15 publications

(9 citation statements)

References 33 publications

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“…Numerous methodologies have been developed recently (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16). As excellently summarized in the latest reviews, to achieve sex hormones profiling, most, if not all, of the published methods introduced liquid-liquid extraction (LLE) or solid phase extraction (SPE) for sample preparation (17,18).…”

Section: Introductionmentioning

confidence: 99%

“…In modern clinical laboratories, LC-MS/MS has become the preferred platform for analyzing endogenous sex hormones ( 4 ). Numerous methodologies have been developed recently ( 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ). As excellently summarized in the latest reviews, to achieve profiling of sex hormones, most, if not all, of the published methods introduced liquid-liquid extraction (LLE) or solid phase extraction (SPE) for sample preparation ( 17 , 18 ).…”

mentioning

confidence: 99%

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Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis

Peng

Yang

et al. 2022

Journal of Lipid Research

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis

Peng

Yang

et al. 2022

Journal of Lipid Research

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“…Corticosterone is an endogenous steroid hormone secreted by the adrenal glands and gonads. 42 Although corticosterone is present at low concentrations, it plays a critical part in regulating many physiological and pathological processes in vivo. Many previous studies have shown that the high corticosterone level in plasma increases the risk of diseases, including cardiovascular and cerebrovascular diseases, diabetes, and its complications.…”

Section: Discussionmentioning

confidence: 99%

“…Corticosterone is an endogenous steroid hormone secreted by the adrenal glands and gonads . Although corticosterone is present at low concentrations, it plays a critical part in regulating many physiological and pathological processes in vivo.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Profiling of Carbonyl Compounds for Unveiling Protective Mechanisms of Pueraria lobata against Diabetic Nephropathy by UPLC-Q-Orbitrap HRMS/MS Analysis

Zhang

Guo

Zhuang

et al. 2021

J. Agric. Food Chem.

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Carbonyl compounds play a critical role in the pathogenesis of diabetic nephropathy (DN). Pueraria lobata (PL), also known as “Kudzu”, is a widely consumed functional food or nutraceutical and has shown promise in the prevention of diabetes and complications such as DN. To explore the beneficial effects and the underlying mechanisms of PL against DN, a new strategy for in-depth metabolic profiling of carbonyl compounds in DN mice plasma by chemical derivatization combined with UPLC-Q-Orbitrap high-resolution mass spectrometry (HRMS)/MS analysis was developed for the first time. Pharmacological evaluation revealed that PL extracts containing a total of 73 identified compounds could ameliorate kidney injury and regulate abnormal glycolipid metabolism. In metabolomics analysis, 19 carbonyl compounds with significant differences were identified between DN mice and normal mice. Moreover, 12 metabolites had a tendency to return to normal levels after PL treatment. Overall, PL exerts beneficial effects on DN by regulating abnormal glycolipid metabolism and carbonyl stress, and endogenous carbonyl compounds might serve as potential biomarkers for DN.

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“…Concerning the hydrophobicity of the DES, the present study has selected menthol and lauric acid as the hydrogen bond acceptor (HBA) The analytical methods used to assess natural steroid hormones in life sciences (bioanalytics, ecology, medicine, and pharmacology) have to overcome challenges such as the low concentration of these compounds and the presence of interfering substances in biological matrices. Hyphenated techniques such as high-performance liquid chromatography (HPLC) or gas chromatography (GC) coupled with mass spectrometry (MS) [6][7][8][9][10][11] are commonly used to achieve high selectivity and sensitivity. However, sample preparation steps, including solid-phase extraction (SPE) [12,13], liquid-liquid extraction (LLE) [14], or derivatization [15], are required to enhance analyte stability or detectability.…”

Section: β-Estradiol (Bes)mentioning

confidence: 99%

Solvent Bar Microextraction Method Based on a Natural Deep Eutectic Solvent and Multivariate Optimization for Determination of Steroid Hormones in Urine and Water

AL-Hashimi,

Abed Alfattah,

El-Barghouthi

et al. 2024

Applied Sciences

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Steroid hormones may pose potential risks to both human health and wildlife, primarily through the consumption of medication or polluted food and water; efforts are being made to monitor their levels in the human body and regulate and minimize their releases to the environment. In this study, a simple and environmentally friendly sample preparation method was developed to simultaneously determine three steroid hormones in urine and water samples. A monoterpene (menthol) and a fatty acid (lauric acid) were combined in various ratios to form a hydrophobic deep eutectic (HDE) solvent as an extraction solvent in solvent bar microextraction (SBME). Using a univariate strategy, a menthol-to-lauric acid HDE ratio of 4:1 and a pH 7 of the sample solution resulted in the highest extraction efficiency (EE%) of the selected steroids. The computational methods have been employed to predict a 4:1 HDE interaction with chosen steroids. Additionally, chemometric approaches suggested that the optimal extraction conditions involved HDEs as extract solvent confined within three SBME devices directly immersed into a 20 mL sample solution with a 30 min extraction time, followed by ultrasonication within 200 μL of elution solvent for a 5 min elution time. Under optimized conditions, the method calibration graph for the spiked selected steroids in the water and urine samples showed good linearity with R2 ≥ 0.994 with limits of detection/quantification lower than 0.40/1.35 μg L−1 and repeatability/reproducibility (RSD%, n = 5) lower than 5.09/7.11. The developed method allows a safe, rapid, and reliable analysis of three steroid hormones in human urine and water samples without using toxic volatile organic solvents.

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Development and validation of a sensitive LC-MS/MS method for simultaneous quantification of thirteen steroid hormones in human serum and its application to the study of type 2 diabetes mellitus

Cited by 15 publications

References 33 publications

Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis

Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis

Metabolic Profiling of Carbonyl Compounds for Unveiling Protective Mechanisms of Pueraria lobata against Diabetic Nephropathy by UPLC-Q-Orbitrap HRMS/MS Analysis

Solvent Bar Microextraction Method Based on a Natural Deep Eutectic Solvent and Multivariate Optimization for Determination of Steroid Hormones in Urine and Water

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