Development and validation of a combined method for the biomonitoring of omega-3/-6 fatty acids and conjugated linoleic acids in different matrices from human and nutritional sources

Böcking, Christian; Nockher, Wolfgang Andreas; Schreiner, Matthias; Renz, Harald; Pfefferle, Petra Ina

doi:10.1515/cclm.2010.350

Cited by 13 publications

(13 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The method for extraction and derivation of fatty acids was adapted from Böcking et al [22]. The fatty acid methyl esters were analyzed using a gas chromatography-mass spectrometry (GLC-MS) system: Focus GC apparatus with ion trap ITQ 700 (Thermo) and Trace GC with TSQ Quantum XLS triple quadrupole (Thermo).…”

Section: Methodsmentioning

confidence: 99%

The profile of polyunsaturated fatty acids in juvenile idiopathic arthritis and association with disease activity

et al. 2017

View full text Add to dashboard Cite

We investigated the association between dietary intake of n-3 and n-6 polyunsaturated fatty acids (PUFAs), serum profiles, and immune and inflammatory markers in juvenile idiopathic arthritis (JIA) in relation to onset, activity, and duration. A total of 66 JIA patients and 42 controls were included. Serum PUFA levels were assessed by gas-liquid chromatography-mass spectrometry, a dietary intake by 7-day dietary record method, and IL-6, IL-10, and IL-17A levels using ELISA. Dietary PUFA intake did not differ between the JIA group and controls. Intakes of n-6 and n-3 PUFA and serum levels were not associated. Levels of total n-6 PUFA and linoleic acid (LA) were higher in inactive JIA than in active JIA. Patients with active and short-lasting disease (less than 3 months from diagnosis) had significantly lower levels of arachidonic acid (AA) and docosahexaenoic acid (DHA) than the control. Serum α-linolenic acid (ALA) levels were significantly higher in poly-JIA than in oligo-JIA and in controls. We found significantly higher serum IL-10 levels in JIA than in controls. Serum n-6 and n-3 levels were significantly negatively correlated with active joint count, erythrocyte sedimentation rate, and C-reactive protein and positively with platelet count. Our study presents the low levels of AA and DHA in the active phase of short-lasting JIA, particularly poly-JIA, and the relationship between n-6 and n-3 PUFA and classic markers of inflammation. PUFAs may contribute to the pathogenesis of JIA and support a necessity to identify new targets suitable for successful interventional studies in JIA patients.

show abstract

Section: Methodsmentioning

confidence: 99%

The profile of polyunsaturated fatty acids in juvenile idiopathic arthritis and association with disease activity

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Similar improvements in the detection of fatty acids contained predominantly in phospholipids have been found in previous studies when higher polarity solvents were used. For example, the concentration of a number of fatty acids including C16:0 and C18:0 in beef [30], human plasma [31] and human RBC [10] was significantly higher Fig. 1 Correlation between the concentration of a C18:0, b C18:2n-6, c C18:3n-3 (lg/mL) and d the ratio of omega-6:omega-3 polyunsaturated fatty acids (n-6:n-3 ratio) in the red blood cells (RBC) of sheep following analysis of fatty acids using the one-step extraction and methylation procedure of Lepage and Roy [11] or the two-step procedure including a Folch extraction [7].…”

Section: Discussionmentioning

confidence: 99%

“…The lower detected concentrations of FAME in RBC when the Folch lipid extraction procedure was used could also be due to physical effects of the solvents on RBC membranes. For example, low polarity chloroform may have led to clotting of membrane-bound proteins and a reduction in the ability of the solvents to extract membrane bound fatty acids [10]. The Folch extraction procedure may need to be undertaken if individual lipid fractions need to be separated, therefore, it may be necessary to improve the extraction of fatty acids using this procedure.…”

Section: Discussionmentioning

confidence: 99%

“…The analysis of fatty acids in RBC typically involves a two-step procedure, firstly, extraction of lipids and, secondly, saponification and methylation [6]. There are several methods available to extract total lipids from RBC, including the Folch procedure [7], the modified procedure of Bligh and Dyer using altered solvent ratios and volumes [8] and procedures using less toxic solvents, including isopropanol [9,10]. Lipid samples then have to be methylated separately, which lengthens the time required for analysis.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Improved Extraction of Saturated Fatty Acids but not Omega‐3 Fatty Acids from Sheep Red Blood Cells Using a One‐Step Extraction Procedure

Clayton

Gulliver

Piltz

et al. 2012

Lipids

View full text Add to dashboard Cite

Several methods are available to extract total lipid and methylate fatty acids from a range of samples including red blood cells (RBC). Fatty acid analysis of human RBC can be undertaken using a two-step extraction and methylation or a combined one-step extraction and methylation procedure. The lipid composition of sheep RBC differs significantly from that of humans and may affect their extraction. The purpose of the current study was to examine the efficiency of extraction of lipid and detection of fatty acids from sheep RBC using a one-step procedure. Fatty acids were analysed using a one-step extraction and methylation procedure using methanol:toluene and acetyl chloride in comparison with a two-step procedure involving extraction of lipid using chloroform:methanol and separate methylation. Concentrations of saturated fatty acids including C16:0 and C18:0 were significantly higher (42.6 and 33.9 % respectively) following extraction using the one-step procedure compared with the two-step procedure. However, concentrations of some polyunsaturated fatty acids, including C20:5n-3 and C22:6n-3 were not significantly different between either procedure. The improved detection of fatty acids may be related to the ability of different solvents to extract different lipid fractions. The differential extraction of lipids and detection of fatty acids from sheep RBC may have important implications in studies examining the effect of dietary treatment on the possible health benefits of fatty acids.

show abstract

“…Blood samples were taken and a panel of different FAs in serum and erythrocyte membranes was assessed as described by Böcking et al [24]. …”

Section: Methodsmentioning

confidence: 99%

Bioavailability and Allergoprotective Capacity of Milk-Associated Conjugated Linoleic Acid in a Murine Model of Allergic Airway Inflammation

Böcking

Harb²,

Ege³

et al. 2014

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

Background: Cross-sectional epidemiological studies have demonstrated that farm milk from traditional farm settings possesses allergoprotective properties. Up to now, it has not been clarified which milk ingredient is responsible for protection against allergic diseases. As farm milk is rich in conjugated linoleic acids (CLA), it is hypothesized that this n-3 polyunsaturated fatty acid family contributes to the allergoprotective capacity of farm milk. We aim to prove this hypothesis in a murine model of allergic airway inflammation. Methods: To prove the bioavailability and allergoprotective capacity of milk-associated CLA in a standardized protocol, milk batches that differed significantly in terms of their CLA content were spray dried and incorporated into a basic diet by substituting the regular sunflower fat fraction. Initially, the milk CLA uptake from the diet was monitored via measurement of the CLA content in plasma and erythrocyte membranes obtained from supplemented mice. To determine whether a milk CLA-enriched diet possesses allergoprotective properties, female Balb/c mice were fed the milk CLA-enriched diet ahead of sensitization and a challenge with ovalbumin (OVA) and the parameters of airway inflammation and eisosanoid pattern were measured. Results: In animals, supplementation with a diet rich in milk CLA resulted in elevated CLA levels in plasma and erythrocyte membranes, indicating bioavailability of milk fatty acids. Though membrane-associated phospholipid patterns were affected by supplementation with milk CLA, this application neither reduced the hallmarks of allergic airway inflammation in sensitized and OVA-challenged mice nor modified the eiconsanoid pattern in the bronchoalveolar lavage fluid of these animals. Conclusion: Milk-associated CLA was not capable of preventing murine allergic airway inflammation in an animal model of OVA-induced allergic airway inflammation.

show abstract

Development and validation of a combined method for the biomonitoring of omega-3/-6 fatty acids and conjugated linoleic acids in different matrices from human and nutritional sources

Cited by 13 publications

References 33 publications

The profile of polyunsaturated fatty acids in juvenile idiopathic arthritis and association with disease activity

The profile of polyunsaturated fatty acids in juvenile idiopathic arthritis and association with disease activity

Improved Extraction of Saturated Fatty Acids but not Omega‐3 Fatty Acids from Sheep Red Blood Cells Using a One‐Step Extraction Procedure

Bioavailability and Allergoprotective Capacity of Milk-Associated Conjugated Linoleic Acid in a Murine Model of Allergic Airway Inflammation

Contact Info

Product

Resources

About