Development and validation of a method for the determination of folic acid in different pharmaceutical formulations using derivative spectrophotometry

Abstract

Folic acid is a B complex water-soluble vitamin that is essential to humans, and its deficiency can cause problems including congenital malformations in the fetus as well as heart disease. Most countries affected by diseases associated with a lack of folic acid now supplement foods with the vitamin. There is therefore a need for the development of new analytical procedures able to determine folic acid present in different matrices. This work describes the development of zero order and first order derivative spectrophotometric methods for the determination of folic acid in different pharmaceutical formulations, using 0.1 mol L -1 NaOH as solvent. The methods are shown to be simple, selective, and robust. Good linearity was achieved, with correction coefficients ≥0.9996 and limits of detection and quantification ranging from 0.64 to 0.75 and from 1.80 to 2.85 mg L -1 , respectively. Recoveries of 98-104% were obtained in accuracy tests, and precision (as RSD) was between 0.2 and 4.8%. The methods can be used in routine analyses for quality control purposes, offering an alternative to the procedures already reported in the literature.Uniterms: Folic acid/pharmaceutical formulations. Folic acid/pharmaceutical formulations/validation method. Folic acid/formulations/derivative spectrophotometry.

Selenium/Chitosan-Folic Acid Metal Complex Ameliorates Hepatic Damage and Oxidative Injury in Male Rats Exposed to Sodium Fluoride

“…In addition, Se/chitosan-folic acid demonstrated an absorption peak between 265 nm and 325 nm, confirming the presence of folic acid [30]. Pure folic acid has shown absorption peaks at wavelengths of 256 nm, 284 nm, and 366 nm [31]. The main differences observed between Se/chitosan-folic acid and the solutions of folic acid confirmed the complexation of Se/chitosan and folic acid.…”

Synthesis and characterization of Zinc/Chitosan-Folic acid complex

“…Furthermore, ZnS/CS-FA showed a well distinguishable absorption peak in the range of 260 nm–320 nm which indicates the presence of folic acid as seen in the literature ( Pujana et al., 2014 ). Pure folic acid solutions were reported to have absorption peaks at 255.5 nm, 283.0 nm and 365.5 nm wavelengths ( Ribeiro et al., 2016 ) and the differences observed in the spectrum of ZnS/CS-FA compared to that of pure folic acid solutions can be attributed to the interactions between ZnS/CS and folic acid.

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pH assists simultaneous determination of folic acid and vitamin D3in biological fluids using a novel Tb3+–acyclovir optical biosensor

“…The results obtained from the proposed spectrouorometric technique was compared with obtained from other previously reported methods [51][52][53][54][55][56][57][58][59][60][61] assuring the applicability, accuracy and precision of the proposed method as presented in Table 4.…”