Development and validation of a chemiluminescent western blot assay for glanders (Burkholderia mallei) serodetection
Juanita F. Grause,
Mandy C. Elschner,
Nicholas A. Ledesma
et al.
Abstract:Glanders, caused by Burkholderia mallei, is a zoonotic disease of equids. Serologic testing for glanders is required by disease-free countries before international movement of equids. The World Organisation for Animal Health Terrestrial Manual recommends the complement fixation test (CFT) for clearance of individual animals for movement, but the CFT is prone to false-positive results. A colorimetric western blot (WB) assay was developed and validated to resolve false-positive CFT results; however, that assay i… Show more
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