Development and validation of a UHPLC-ESI-QTOF mass spectrometry method to analyze opines, plant biomarkers of crown gall or hairy root diseases

Padilla, Rosa; Gaillard, Vincent; Le, Thao Nhi; Bellvert, Floriant; Chapulliot, David; Nesme, Xavier; Dessaux, Yves; Vial, Ludovic; Lavire, Céline; Kerzaon, Isabelle

doi:10.1016/j.jchromb.2020.122458

Cited by 12 publications

(8 citation statements)

References 62 publications

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“…Aiming at high-throughput quantitation and low detection limits, UHPLC–MS/MS is still the method of choice demonstrated through the newly created HILIC-UHPLC–MS/MS MRM method for the quantitation of opines. Since opines play a role not only in seafoods but also in the plant world, where they are present in much lower concentrations, and the quantitation so far only relies on enzymatic assays, LC–MS methods targeting derivatized opines, or external calibrated LC–MS methods, this new method fills a gap by analyzing opines fast, sensitive, without derivatization and under application of stable isotope-labeled internal standards.…”

Section: Resultsmentioning

confidence: 99%

Identification and Quantitation of Taste-Active Compounds in Dried Scallops by Combined Application of the Sensomics and a Quantitative NMR Approach

Dirndorfer

Hammerl

Kitajima

et al. 2021

J. Agric. Food Chem.

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Application of the sensomics concept on dried scallops, a Japanese specialty produced from the adductor muscle of scallops, revealed after activity-guided fractionation with subsequent (comparative) taste dilution analyses besides nucleotides, amino acids, organic acids, and inorganic ions, the presence of taste-modulating quaternary ammonium compounds and opines in highly taste-active fractions. In order to recreate the taste of dried scallops, two independent quantitation approaches were applied and compared. The first approach used multiple targeted UHPLC−MS/MS and high-performance ion chromatography methods. Besides already established quantitation methods for basic taste compounds, a new HILIC-UHPLC−MS/MS MRM method for the quantitation of chromatographically challenging opines, using synthesized stable isotope-labeled standards, was developed. Furthermore, a qHNMR approach was applied, enabling a direct identification and quantitation of organic taste compounds in a food extract without prior fractionation using a reference 1 H NMR database. Both methods yielded similar quantitative results of taste-active compounds in dried scallop extracts and subsequent taste recombination experiments based on these data were able to recreate the taste of dried scallops.

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Section: Resultsmentioning

confidence: 99%

Identification and Quantitation of Taste-Active Compounds in Dried Scallops by Combined Application of the Sensomics and a Quantitative NMR Approach

Dirndorfer

Hammerl

Kitajima

et al. 2021

J. Agric. Food Chem.

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“…Different strains of R. rhizogenes have abilities to inoculate plant tissues based on the opine type that they produce in the transformed tissue. Agropine-type strains, such as ATCC 15834, generally have more ability to inoculate plant tissues (Padilla et al 2021). However, this is not always true as strain A13 (non-agropine) induced more root hairs in leaf microspecimens of Dracocephalum kotschyi than strain LBA9402 (agropine) (Shara et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Effect of Acetosyringone, Sucrose and Nutrients on Transgenic Hairy Root Induction in Chenopodium quinoa using different Rhizobium rhizogenesstrains

Massah

Rabiei

2023

Preprint

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Quinoa is a plant with special nutritional and biological properties and tolerant in strict environmental conditions, which can be considered to a strategic product for the future food supply of mankind, as a pseudocereal. Quinoa root is rich in bioactive compounds with antimicrobial, antioxidant activity and diverse medicinal and nutritional properties that can have a potential for commercialization. The use of cultivation and propagation techniques in controlled laboratory conditions, such as the induction of differentiated tissues of hairy roots, allows the production of plants and the rapid and massive access to secondary metabolites. In this research, the induction of hairy roots was evaluated using four Rhizobium rhizogenes strains, including A4, A7, 11325, and ATCC 15834, in quinoa and the effect of different concentrations of acetosyringone and sucrose and removal of macro elements in the culture medium in increasing the rooting performance of R. rhizogenes was investigated. The results showed the different effects of the mutual effect of different concentrations of sucrose and acetosyringone, as well as the commonly used elements of the half strength MS culture medium on the biomass of hairy roots. The most optimal conditions for the induction of hairy roots culture were obtained in half strength MS culture medium, containing 30 g/l sucrose and 1 mM acetosyringone and without NH4NO3 salt. This efficient protocol provides a basis for future studies to be able to use the induced hairy root as a source of valuable secondary metabolites of quinoa root and create a new approach for exploiting the nutritional, medicinal and health compounds of the quinoa plant.

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“…In the later stage, the root absorption function of the diseased plants is hindered, which affects the plant growth rate and tree vigor. In severe cases, it can cause the death of the whole tree [ 46 , 47 ]. This event reduces the ornamental quality and commodity value of this fruit plant.…”

Section: Discussionmentioning

confidence: 99%

First Report of Crown Gall of Kiwifruit (Actinidia deliciosa) Caused by Agrobacterium fabacearum in China and the Establishment of Loop-Mediated Isothermal Amplification Technique

Shi

Zhang

et al. 2021

IJMS

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Kiwifruit is moderately sweet and sour and quite popular among consumers; it has been widely planted in some areas of the world. In 2019, the crown gall disease of kiwifruit was discovered in the main kiwifruit-producing area of Guizhou Province, China. This disease can weaken and eventually cause the death of the tree. The phylogeny, morphological and biological characteristics of the bacteria were described, and were related to diseases. The pathogenicity of this species follows the Koch hypothesis, confirming that A. fabacearum is the pathogen of crown gall disease of kiwifruit in China. In this study, Loop-mediated isothermal amplification (LAMP) analysis for genome-specific gene sequences was developed for the specific detection of A. fabacearum. The detection limit of the LAMP method is 5 × 10−7 ng/μL, which has high sensitivity. At the same time, the amplified product is stained with SYBR Green I after the reaction is completed, so that the amplification can be detected with the naked eye. LAMP analysis detected the presence of A. fabacearum in the roots and soil samples of the infected kiwifruit plant. The proposed LAMP detection technology in this study offers the advantages of ease of operation, visibility of results, rapidity, accuracy and high sensitivity, making it suitable for the early diagnosis of crown gall disease of kiwifruit.

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Development and validation of a UHPLC-ESI-QTOF mass spectrometry method to analyze opines, plant biomarkers of crown gall or hairy root diseases

Cited by 12 publications

References 62 publications

Identification and Quantitation of Taste-Active Compounds in Dried Scallops by Combined Application of the Sensomics and a Quantitative NMR Approach

Identification and Quantitation of Taste-Active Compounds in Dried Scallops by Combined Application of the Sensomics and a Quantitative NMR Approach

Effect of Acetosyringone, Sucrose and Nutrients on Transgenic Hairy Root Induction in Chenopodium quinoa using different Rhizobium rhizogenesstrains

First Report of Crown Gall of Kiwifruit (Actinidia deliciosa) Caused by Agrobacterium fabacearum in China and the Establishment of Loop-Mediated Isothermal Amplification Technique

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