Development and validation of a porcine artificial colonic mucus model reflecting the properties of native colonic mucus in pigs

Barmpatsalou, Vicky; Rodler, Agnes; Jacobson, Magdalena; Karlsson, Εva; Lomstein, Betty Pedersen; Bergström, Christel A S

doi:10.1016/j.ejps.2022.106361

Cited by 4 publications

(5 citation statements)

References 64 publications

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“…The pore size descriptor used was Feret’s minimum diameter, which refers to the shortest distance between two parallel tangents of a pore [44-46]. This parameter is widely used to describe sizes and distribution of pores in SEM mucus samples.…”

Section: Resultsmentioning

confidence: 99%

“…Holes on the edges of the images were excluded from analysis to avoid artifacts. The maximal and minimal Feret diameter [44-46] of each pore was automatically quantified and manually verified. The distribution of minimal Feret diameters of all pores per sample was visualized in a violin plot with the mean value and the single standard deviation indicated as lines within the violin plot.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Comprehensive characterization of the viscoelastic properties of Bovine Submaxillary Mucin (BSM) hydrogels and the effect of additives

Rulff,

Schmidt,

Wei

et al. 2024

Preprint

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This study presents a comprehensive characterization of the viscoelastic and structural properties of Bovine Submaxillary Mucin (BSM), a good biomaterial model system for mucins and mucus in general. We conducted concentration studies of BSM and examined the effects of various additives - sodium chloride, calcium chloride, lysozyme, and DNA - on its rheological behavior. Through oscillatory and shear flow macrorheological experiments, we established a detailed correlation between the concentration of BSM and its viscoelastic properties, uncovering changes in the rheological moduli with the addition of sodium and calcium chloride, particularly at higher concentrations. This highlights the mucins responsiveness to ionic strength and composition variations. The presence of lysozyme and DNA further influenced the rheological characteristics of BSM, adding a new dimension to our understanding of mucin behavior. The rheological spectra could be well-described by a Fractional Kelvin-Voigt Model (FKVM), which allows to describe the complete behavior with a minimum of model parameters. A detailed proteomics analysis provided insight into the molecular interactions within BSM, enhancing our understanding of its structural intricacies. Complementing this, cryo-scanning electron microscopy (cryo-SEM) was employed to visualize the microstructural alterations and network properties in relation to the rheological data. By elucidating the complex interplay between mucin concentration, environmental conditions, and viscoelastic properties, this research significantly contributes to the field of mucus research and lays an important basis for future studies and advanced applications of numerous mucin-based biomaterials.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Comprehensive characterization of the viscoelastic properties of Bovine Submaxillary Mucin (BSM) hydrogels and the effect of additives

Rulff,

Schmidt,

Wei

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

“…The pore size descriptor used was Feret’s minimum diameter, which refers to the shortest distance between two parallel tangents of a pore. − This parameter is widely used to describe the sizes and distribution of pores in SEM mucus samples. The analysis showed no significant differences between the two BSM batches in terms of their pore size.…”

Section: Resultsmentioning

confidence: 99%

“…Holes on the edges of the images were excluded from analysis to avoid artifacts. The maximal and minimal Feret diameter − of each pore was automatically quantified and manually verified. The distribution of minimal Feret diameters of all pores per sample was visualized in a violin plot with the mean value and the single standard deviation indicated as lines within the violin plot.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive Characterization of the Viscoelastic Properties of Bovine Submaxillary Mucin (BSM) Hydrogels and the Effect of Additives

Rulff,

Schmidt,

Wei

et al. 2024

Biomacromolecules

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This study presents a comprehensive characterization of the viscoelastic and structural properties of bovine submaxillary mucin (BSM), which is widely used as a commercial source to conduct mucus-related research. We conducted concentration studies of BSM and examined the effects of various additives, NaCl, CaCl 2 , MgCl 2 , lysozyme, and DNA, on its rheological behavior. A notable connection between BSM concentration and viscoelastic properties was observed, particularly under varying ionic conditions. The rheological spectra could be well described by a fractional Kelvin−Voigt model with a minimum of model parameters. A detailed proteomics analysis provided insight into the protein, especially mucin composition within BSM, showing MUC19 as the main component. Cryo-scanning electron microscopy enabled the visualization of the porous BSM network structure. These investigations give us a more profound comprehension of the BSM properties, especially those pertaining to viscoelasticity, and how they are influenced by concentration and environmental conditions, aspects relevant to the field of mucus research.

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“…[ 63 ] Like PVA, polyacrylic acid (PAA), when supplemented with porcine MUC2 or MUC3, bovine serum albumin (BSA) and lipids (cholesterol and phosphatidylcholine) can form artificial porcine colonic mucus with comparable gelation, viscoelasticity and shear‐thinning behavior to native intestinal mucus isolated from porcine intestine. [ 87 ] Biosimilar mucus models can also be generated by reconstituting porcine mucin with additives such as lipids and protein components of native mucus and applying the artificial mucus to a transwell membrane. [ 88 ] Such biosimilar mucus exhibits improve gelation in comparison to mucin solution in buffer, indicating that biosimilar mucus may be a more accurate model of the innermost, denser intestinal mucus layer.…”

Section: Synthetic Mimics Of Mucus and The Mucosal Layermentioning

confidence: 99%

Mucosa‐Mimetic Materials for the Study of Intestinal Homeostasis and Disease

Donahue

Sahoo

Rudolph

et al. 2023

Adv Healthcare Materials

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Mucus is a viscoelastic hydrogel that lines and protects the epithelial surfaces of the body that houses commensal microbiota and functions in host defense against pathogen invasion. As a first‐line physical and biochemical barrier, intestinal mucus is involved in immune surveillance and spatial organization of the microbiome, while dysfunction of the gut mucus barrier is implicated in several diseases. Mucus can be collected from a variety of mammalian sources for study, however, established methods are challenging in terms of scale and efficiency, as well as with regard to rheological similarity to native human mucus. Therefore, there is a need for mucus‐mimetic hydrogels that more accurately reflect the physical and chemical profile of the in vivo human epithelial environment to enable the investigation of the role of mucus in human disease and interactions with the intestinal microbiome. This review will evaluate the material properties of synthetic mucus mimics to date designed to address the above need, with a focus toward an improved understanding of the biochemical and immunological functions of these biopolymers related to utility for research and therapeutic applications.

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Development and validation of a porcine artificial colonic mucus model reflecting the properties of native colonic mucus in pigs

Cited by 4 publications

References 64 publications

Comprehensive characterization of the viscoelastic properties of Bovine Submaxillary Mucin (BSM) hydrogels and the effect of additives

Comprehensive characterization of the viscoelastic properties of Bovine Submaxillary Mucin (BSM) hydrogels and the effect of additives

Comprehensive Characterization of the Viscoelastic Properties of Bovine Submaxillary Mucin (BSM) Hydrogels and the Effect of Additives

Mucosa‐Mimetic Materials for the Study of Intestinal Homeostasis and Disease

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