Development and validation of a rapid ultra-high performance liquid chromatography diode array detector method for Verbena officinalis L.

Gibitz-Eisath, Nora; Eichberger, Miriam; Gruber, Regina; Sturm, Sonja; Stuppner, Hermann

doi:10.1016/j.jpba.2018.07.047

Cited by 6 publications

(9 citation statements)

References 19 publications

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“…In a previous study, presented by our institution, a UHPLC-DAD method for the quantitative quality control of Verbena herba has been developed [29]. The optimized conditions are described in Section 2.3.2.…”

Section: Comparison Of Uhpsfc and Uhplc Methodsmentioning

confidence: 99%

“…Voucher specimens are deposited at the Institute of Pharmacy, University of Innsbruck. The reference compounds hastatoside (1), verbenalin (2), apigenin-7-O-glucuronide (3), cistanoside D (4), luteolin-7-O-glucuronide (5), apigenin-7-O-diglucuronide (6), verbascoside (7), and luteolin-7-Odiglucuronide (8) were isolated and structurally characterized at the Institute of Pharmacy, University of Innsbruck [29]. Purity of all reference compounds was ≥ 93% as determined by UHPLC-DAD.…”

Section: Chemicals and Materialsmentioning

confidence: 99%

“…Sample preparation followed a published protocol [29]. Briefly, ground Verbenae herba plant material (VO-1 to VO-3) was frozen in liquid nitrogen and homogenized with an analytical ball mill (Mikro-Dismembrator, Sartorius, Göttingen, Germany).…”

Section: Sample Preparationmentioning

confidence: 99%

“…UHPLC-DAD analysis was performed according to a published protocol [29]. The utilized system was an Agilent 1290 series HPLC instrument, equipped with a quaternary pump, autosampler, column oven, and a photodiode array detector (Agilent, Waldbronn, Germany).…”

Section: Uhplc Analysismentioning

confidence: 99%

“…Subsequently, the optimized UHPSFC-DAD assay was validated according to the ICH guidelines and applied to the quantitation of the major compounds in Verbenae herba extracts. Finally, a head-to-head comparison with a recently published UHPLC-DAD [29] method was carried out in a cross-validation experiment to demonstrate quantitative analytical equivalence of these highly orthogonal separation techniques.…”

Section: Introductionmentioning

confidence: 99%

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Towards eco‐friendly secondary plant metabolite quantitation: Ultra high performance supercritical fluid chromatography applied to common vervain (Verbena officinalis L.)

Gibitz-Eisath

Eichberger

Gruber

et al. 2019

J of Separation Science

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This report presents the first ultra high performance supercritical fluid chromatography diode array detector based assay for simultaneous determination of iridoid glucosides, flavonoid glucuronides, and phenylpropanoid glycosides in Verbena officinalis (Verbenaceae) extracts. Separation of the key metabolites was achieved in less than 7 min on an Acquity UPC2 Torus Diol column using a mobile phase gradient comprising subcritical carbon dioxide and methanol with 0.15% phosphoric acid. Method validation for seven selected marker compounds (hastatoside, verbenalin, apigenin‐7‐O‐glucuronide, luteolin‐7‐O‐glucuronide, apigenin‐7‐O‐diglucuronide, verbascoside, and luteolin‐7‐O‐diglucuronide) confirmed the assay to be sensitive, linear, precise, and accurate. Head‐to‐head comparison to an ultra high performance liquid chromatography comparator assay did prove the high orthogonality of the methods. Quantitative result equivalence was evaluated by Passing‐Bablok‐correlation and Bland‐Altman‐plot analysis. This cross‐validation revealed, that one of the investigated marker compound peaks was contaminated in the ultra high performance liquid chromatography assay by a structurally related congener. Taken together, it was proven that the ultra high performance supercritical fluid chromatography instrument setup with its orthogonal selectivity is a true alternative to conventional reversed phase liquid chromatography in quantitative secondary metabolite analysis. For regulatory purposes, assay cross‐validation with highly orthogonal methods seems a viable approach to avoid analyte overestimation due to coeluting, analytically indistinguishable contaminants.

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Section: Comparison Of Uhpsfc and Uhplc Methodsmentioning

confidence: 99%

Section: Chemicals and Materialsmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Section: Uhplc Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Towards eco‐friendly secondary plant metabolite quantitation: Ultra high performance supercritical fluid chromatography applied to common vervain (Verbena officinalis L.)

Gibitz-Eisath

Eichberger

Gruber

et al. 2019

J of Separation Science

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Chemical constituents from Phlomis bovei Noë and their chemotaxonomic significance

Khitri

Smati

Mitaine-Offer

et al. 2020

Biochemical Systematics and Ecology

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Verbena officinalis (Common Vervain) – A Review on the Investigations of This Medicinally Important Plant Species

et al. 2020

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Verbena officinalis (common vervain) is a medicinal plant species widely distributed in the world and commonly used in folk medicine of different countries, including traditional Chinese medicine. Monographs on “Verbenae herba” have been included in the European Pharmacopoeia since 2008, and in the Chinese Pharmacopoeia since 1995. This work presents botanical characteristics of this species. It reviews the current knowledge of its chemical composition, which is a rich source mostly of iridoids, phenylpropanoid glycosides, phenolic acids, flavonoids, terpenoids, and essential oil. A large part of this article summarizes traditional medicinal uses and professional pharmacological in vitro and in vivo studies that prove new important applications, e.g., antioxidant, antimicrobial, anti-inflammatory, neuroprotective anticancer, analgesic, or anticonvulsant of verbena herb extracts and individual metabolites. Moreover, emphasis is put on the use of V. officinalis in the food and cosmetics industries, especially due to its antioxidant, antibacterial, and anti-inflammatory properties, and the presence of essential oil with an attractive fragrance composition. This paper also presents the state of biotechnological studies of this species.

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Development and validation of a rapid ultra-high performance liquid chromatography diode array detector method for Verbena officinalis L.

Cited by 6 publications

References 19 publications

Towards eco‐friendly secondary plant metabolite quantitation: Ultra high performance supercritical fluid chromatography applied to common vervain (Verbena officinalis L.)

Towards eco‐friendly secondary plant metabolite quantitation: Ultra high performance supercritical fluid chromatography applied to common vervain (Verbena officinalis L.)

Chemical constituents from Phlomis bovei Noë and their chemotaxonomic significance

Verbena officinalis (Common Vervain) – A Review on the Investigations of This Medicinally Important Plant Species

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