2021
DOI: 10.2147/ijgm.s312633
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Development and Validation of a Simple and Sensitive LC-MS/MS Method for Quantification of Metformin in Dried Blood Spot Its Application as an Indicator for Medication Adherence

Abstract: Metformin (MET), an oral biguanide agent, can improve insulin resistance and decrease hepatic glucose production, leading to a reduction in blood-sugar levels. The objective of the present study was to develop and validate simple and rapid LC-MS/MS method for analysis of MET in dried blood spot (DBS) sample for patient monitoring studies purposes (drug adherence). Methods: The chromatographic separation was achieved with Waters HSS-T3 column using gradient elution of mobile phases of two solvents: 1) solvent A… Show more

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Cited by 6 publications
(1 citation statement)
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“…The coagulation of fresh blood upon contact with the organic working solution made preparing standards and QCs impractical. To make the assay more practical, standards and QCs were prepared by spiking the working solutions directly onto blank DBS spots [27] , [28] . For this study, spots were first punched and placed in 2 mL Eppendorf tubes.…”
Section: Methodsmentioning
confidence: 99%